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dc.creatorRistivojević, Petar
dc.creatorTahir, Ammar
dc.creatorMalfent, Fabian
dc.creatorMilojković-Opsenica, Dušanka
dc.creatorRollinger, Judith M.
dc.date.accessioned2019-05-07T10:47:15Z
dc.date.available2019-05-07T10:47:15Z
dc.date.issued2019
dc.identifier.issn0021-9673
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/2918
dc.description.abstractQuality control is a crucial step in the production of effective and safe herbal remedies. The aim of this study was to develop a new, simple, and high throughput procedure for the quality assessment of herbal drugs using a high-performance thin-layer chromatography (HPTLC)/bioautography and UPLC–MS/MS approach combined with chemometrics. This was exemplarily shown for Morus alba L. root bark (sāng bái pí; SBP). Bioautography assays were developed for the identification of constituents with radical scavenging (DPPH assay) and antimicrobial activities (Bacillus subtilis, Escherichia coli) of 18 different M. alba samples, which was supported by UPLC–MS/MS analysis. Further, the combination of bioautography and chemometrics identified those samples with the most bioactive constituents. Plant materials collected from Serbia (11 samples) showed higher both radical scavenging and antimicrobial activities compared to samples provided from China (7 samples). Principal component analysis (PCA) confirmed the discrimination of geographically different samples and recognized their main markers responsible for differences between Serbian and Chinese samples. Most importantly for quality assessment, the combined HPTLC/bioautography and UPLC–MS/MS approach not only allowed for a fast chemical profiling of the investigated samples and their unambiguous identification, but also for the disclosure of major and minor bioactive constituents present in SBP.
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172017/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Chromatography A
dc.subjectBioautography
dc.subjectHPTLC
dc.subjectPattern recognition
dc.subjectQuality control
dc.subjectsāng bái pí
dc.subjectUPLC–MS/MS
dc.titleHigh-performance thin-layer chromatography/bioautography and liquid chromatography-mass spectrometry hyphenated with chemometrics for the quality assessment of Morus alba samples
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРоллингер, Јудитх М.; Ристивојевић, Петар; Тахир, Aммар; Малфент, Фабиан; Милојковић-Опсеница, Душанка.;
dc.citation.volume1594
dc.citation.spage190
dc.citation.epage198
dc.identifier.wos000467665400020
dc.identifier.doi10.1016/j.chroma.2019.02.006
dc.citation.rankM21~
dc.description.otherSupplementary material : [http://cherry.chem.bg.ac.rs/handle/123456789/2919]
dc.description.otherPeer-reviewed manuscript: [http://cherry.chem.bg.ac.rs/handle/123456789/2944]
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85061118029


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