Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India)
Autori
Rajan, SanchoPattanaik, Anwesha
Kumaresan, Venkatesh
Bhatt, Prasanth
Gunasekaran, Senthilarasu
Arockiaraj, Jesu
Pasupuleti, Mukesh
Beškoski, Vladimir
Chakraborty, Paromita
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Microorganisms capable of using naphthalene as the sole carbon source were isolated from the contaminated sediment of Cooum River. Twenty one isolates were recovered and nine were selected for enrichment due to differences in their morphological characteristics. Out of nine isolates, only four (NS3-SRMND14B, NS14-SRMND14A, NS15-SRMND14D and NS19- -SRMND14E) were capable of completely utilizing naphthalene as the sole source of carbon in carbon free minimal medium (CFMM) supplemented with naphthalene. 16S rDNA sequencing showed that all the four isolates were distantly related to each other and belongs to Bacillus sp. (NS3-SRMND14B), Pseudomonas sp. (NS14-SRMND14A), Cellulosimicrobium sp. (NS15-SRMND14D) and Sphingobacterium sp. (NS19-SRMND14E), respectively. Based on the phylogenetic analysis of 16S rDNA sequencing, the isolate Sphingobacterium sp. (NS19-SRMND14E) has been identified as novel strain. Polymerase chain reaction (PCR) technique showed the presence of naphthalene dioxygena...se (ndo) gene responsible for naphthalene degradation only in the Pseudomonas sp. (NS14-SRMND14A). We observed that the ndo gene is not the only gene responsible for naphthalene degradation. Based on our study, the indigenous microorganisms isolated from Cooum Riverine sediment can be used for bioremediation of the polluted sediment along the Bay of Bengal.
Ključne reči:
16S RNA / Indigenous bacteria / Naphthalene / Ndo gene / Phylogenetic tree / RemediationIzvor:
Journal of the Serbian Chemical Society, 2019, 84, 2, 225-236Izdavač:
- Belgrade : Serbian Chemical Society
Napomena:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3840
DOI: 10.2298/JSC180724088R
ISSN: 0352-5139
WoS: 000460644600009
Scopus: 2-s2.0-85065447047
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Rajan, Sancho AU - Pattanaik, Anwesha AU - Kumaresan, Venkatesh AU - Bhatt, Prasanth AU - Gunasekaran, Senthilarasu AU - Arockiaraj, Jesu AU - Pasupuleti, Mukesh AU - Beškoski, Vladimir AU - Chakraborty, Paromita PY - 2019 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3048 AB - Microorganisms capable of using naphthalene as the sole carbon source were isolated from the contaminated sediment of Cooum River. Twenty one isolates were recovered and nine were selected for enrichment due to differences in their morphological characteristics. Out of nine isolates, only four (NS3-SRMND14B, NS14-SRMND14A, NS15-SRMND14D and NS19- -SRMND14E) were capable of completely utilizing naphthalene as the sole source of carbon in carbon free minimal medium (CFMM) supplemented with naphthalene. 16S rDNA sequencing showed that all the four isolates were distantly related to each other and belongs to Bacillus sp. (NS3-SRMND14B), Pseudomonas sp. (NS14-SRMND14A), Cellulosimicrobium sp. (NS15-SRMND14D) and Sphingobacterium sp. (NS19-SRMND14E), respectively. Based on the phylogenetic analysis of 16S rDNA sequencing, the isolate Sphingobacterium sp. (NS19-SRMND14E) has been identified as novel strain. Polymerase chain reaction (PCR) technique showed the presence of naphthalene dioxygenase (ndo) gene responsible for naphthalene degradation only in the Pseudomonas sp. (NS14-SRMND14A). We observed that the ndo gene is not the only gene responsible for naphthalene degradation. Based on our study, the indigenous microorganisms isolated from Cooum Riverine sediment can be used for bioremediation of the polluted sediment along the Bay of Bengal. PB - Belgrade : Serbian Chemical Society T2 - Journal of the Serbian Chemical Society T1 - Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India) VL - 84 IS - 2 SP - 225 EP - 236 DO - 10.2298/JSC180724088R ER -
@article{ author = "Rajan, Sancho and Pattanaik, Anwesha and Kumaresan, Venkatesh and Bhatt, Prasanth and Gunasekaran, Senthilarasu and Arockiaraj, Jesu and Pasupuleti, Mukesh and Beškoski, Vladimir and Chakraborty, Paromita", year = "2019", abstract = "Microorganisms capable of using naphthalene as the sole carbon source were isolated from the contaminated sediment of Cooum River. Twenty one isolates were recovered and nine were selected for enrichment due to differences in their morphological characteristics. Out of nine isolates, only four (NS3-SRMND14B, NS14-SRMND14A, NS15-SRMND14D and NS19- -SRMND14E) were capable of completely utilizing naphthalene as the sole source of carbon in carbon free minimal medium (CFMM) supplemented with naphthalene. 16S rDNA sequencing showed that all the four isolates were distantly related to each other and belongs to Bacillus sp. (NS3-SRMND14B), Pseudomonas sp. (NS14-SRMND14A), Cellulosimicrobium sp. (NS15-SRMND14D) and Sphingobacterium sp. (NS19-SRMND14E), respectively. Based on the phylogenetic analysis of 16S rDNA sequencing, the isolate Sphingobacterium sp. (NS19-SRMND14E) has been identified as novel strain. Polymerase chain reaction (PCR) technique showed the presence of naphthalene dioxygenase (ndo) gene responsible for naphthalene degradation only in the Pseudomonas sp. (NS14-SRMND14A). We observed that the ndo gene is not the only gene responsible for naphthalene degradation. Based on our study, the indigenous microorganisms isolated from Cooum Riverine sediment can be used for bioremediation of the polluted sediment along the Bay of Bengal.", publisher = "Belgrade : Serbian Chemical Society", journal = "Journal of the Serbian Chemical Society", title = "Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India)", volume = "84", number = "2", pages = "225-236", doi = "10.2298/JSC180724088R" }
Rajan, S., Pattanaik, A., Kumaresan, V., Bhatt, P., Gunasekaran, S., Arockiaraj, J., Pasupuleti, M., Beškoski, V.,& Chakraborty, P.. (2019). Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India). in Journal of the Serbian Chemical Society Belgrade : Serbian Chemical Society., 84(2), 225-236. https://doi.org/10.2298/JSC180724088R
Rajan S, Pattanaik A, Kumaresan V, Bhatt P, Gunasekaran S, Arockiaraj J, Pasupuleti M, Beškoski V, Chakraborty P. Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India). in Journal of the Serbian Chemical Society. 2019;84(2):225-236. doi:10.2298/JSC180724088R .
Rajan, Sancho, Pattanaik, Anwesha, Kumaresan, Venkatesh, Bhatt, Prasanth, Gunasekaran, Senthilarasu, Arockiaraj, Jesu, Pasupuleti, Mukesh, Beškoski, Vladimir, Chakraborty, Paromita, "Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India)" in Journal of the Serbian Chemical Society, 84, no. 2 (2019):225-236, https://doi.org/10.2298/JSC180724088R . .