Isolation of apigenin from subcritical water extracts: Optimization of the process
Authors
Cvetanović, AleksandraŠvarc-Gajić, Jaroslava
Gašić, Uroš M.
Tešić, Živoslav Lj.
Zengin, Gokhan
Zeković, Zoran P.
Đurović, Saša
Article (Accepted Version)
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Show full item recordAbstract
Apigenin is a phytochemical which has been associated with numerous beneficial health effects. However, due to its low concentration and poor bioavailability, isolation from natural sources is very difficult. Subcritical water (SCW) is an excellent solvent for the extraction of moderately polar and non-polar flavonoids. Carefully balanced moderate hydrolytical potential of SCW was used in this work to liberate apigenin from its bound forms. In this research simultaneous extraction and hydrolysis of apigenin bound forms from chamomile have been performed. Optimal operation parameters for maximum yields of apigenin have been investigated and defined (solvent-to-sample ratio 1:30; agitation rate 3 Hz; pressure 45 bar; temperature 115 degrees C; time 30 min). Apigenin was further isolated in its pure form from water extracts obtained under optimal conditions using column chromatography. The purity of isolated compound was confirmed by spectroscopic analysis. Biological activity of extracts... obtained under optimal conditions were determined as well. In order to identify polyphenolic compounds UHPLC-HESI-MS/MS analysis was performed.
Keywords:
Apigenin / Extraction parameters / Phenols / Subcritical water extraction / UHPLC-HESI-MS/MS analysisSource:
Journal of Supercritical Fluids, 2017, 120, 32-42Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Pharmacologically active substances and products of medicinal/aromatic plants for pharmaceutical use (RS-MESTD-Technological Development (TD or TR)-31013)
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-MESTD-Basic Research (BR or ON)-172017)
Note:
- This is the peer-reviewed version of the following article: Cvetanović, A.; Švarc-Gajić, J.; Gašić, U.; Tešić, Ž.; Zengin, G.; Zeković, Z.; Đurović, S. Isolation of Apigenin from Subcritical Water Extracts: Optimization of the Process. Journal of Supercritical Fluids 2017, 120, 32–42. https://doi.org/10.1016/j.supflu.2016.10.012
DOI: 10.1016/j.supflu.2016.10.012
ISSN: 0896-8446
WoS: 000391075500004
Scopus: 2-s2.0-84994323092
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Cvetanović, Aleksandra AU - Švarc-Gajić, Jaroslava AU - Gašić, Uroš M. AU - Tešić, Živoslav Lj. AU - Zengin, Gokhan AU - Zeković, Zoran P. AU - Đurović, Saša PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3137 AB - Apigenin is a phytochemical which has been associated with numerous beneficial health effects. However, due to its low concentration and poor bioavailability, isolation from natural sources is very difficult. Subcritical water (SCW) is an excellent solvent for the extraction of moderately polar and non-polar flavonoids. Carefully balanced moderate hydrolytical potential of SCW was used in this work to liberate apigenin from its bound forms. In this research simultaneous extraction and hydrolysis of apigenin bound forms from chamomile have been performed. Optimal operation parameters for maximum yields of apigenin have been investigated and defined (solvent-to-sample ratio 1:30; agitation rate 3 Hz; pressure 45 bar; temperature 115 degrees C; time 30 min). Apigenin was further isolated in its pure form from water extracts obtained under optimal conditions using column chromatography. The purity of isolated compound was confirmed by spectroscopic analysis. Biological activity of extracts obtained under optimal conditions were determined as well. In order to identify polyphenolic compounds UHPLC-HESI-MS/MS analysis was performed. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Supercritical Fluids T1 - Isolation of apigenin from subcritical water extracts: Optimization of the process VL - 120 SP - 32 EP - 42 DO - 10.1016/j.supflu.2016.10.012 ER -
@article{ author = "Cvetanović, Aleksandra and Švarc-Gajić, Jaroslava and Gašić, Uroš M. and Tešić, Živoslav Lj. and Zengin, Gokhan and Zeković, Zoran P. and Đurović, Saša", year = "2017", abstract = "Apigenin is a phytochemical which has been associated with numerous beneficial health effects. However, due to its low concentration and poor bioavailability, isolation from natural sources is very difficult. Subcritical water (SCW) is an excellent solvent for the extraction of moderately polar and non-polar flavonoids. Carefully balanced moderate hydrolytical potential of SCW was used in this work to liberate apigenin from its bound forms. In this research simultaneous extraction and hydrolysis of apigenin bound forms from chamomile have been performed. Optimal operation parameters for maximum yields of apigenin have been investigated and defined (solvent-to-sample ratio 1:30; agitation rate 3 Hz; pressure 45 bar; temperature 115 degrees C; time 30 min). Apigenin was further isolated in its pure form from water extracts obtained under optimal conditions using column chromatography. The purity of isolated compound was confirmed by spectroscopic analysis. Biological activity of extracts obtained under optimal conditions were determined as well. In order to identify polyphenolic compounds UHPLC-HESI-MS/MS analysis was performed.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Supercritical Fluids", title = "Isolation of apigenin from subcritical water extracts: Optimization of the process", volume = "120", pages = "32-42", doi = "10.1016/j.supflu.2016.10.012" }
Cvetanović, A., Švarc-Gajić, J., Gašić, U. M., Tešić, Ž. Lj., Zengin, G., Zeković, Z. P.,& Đurović, S.. (2017). Isolation of apigenin from subcritical water extracts: Optimization of the process. in Journal of Supercritical Fluids Elsevier Science Bv, Amsterdam., 120, 32-42. https://doi.org/10.1016/j.supflu.2016.10.012
Cvetanović A, Švarc-Gajić J, Gašić UM, Tešić ŽL, Zengin G, Zeković ZP, Đurović S. Isolation of apigenin from subcritical water extracts: Optimization of the process. in Journal of Supercritical Fluids. 2017;120:32-42. doi:10.1016/j.supflu.2016.10.012 .
Cvetanović, Aleksandra, Švarc-Gajić, Jaroslava, Gašić, Uroš M., Tešić, Živoslav Lj., Zengin, Gokhan, Zeković, Zoran P., Đurović, Saša, "Isolation of apigenin from subcritical water extracts: Optimization of the process" in Journal of Supercritical Fluids, 120 (2017):32-42, https://doi.org/10.1016/j.supflu.2016.10.012 . .