dc.creator | Minić, Simeon L. | |
dc.creator | Radomirović, Mirjana Ž. | |
dc.creator | Savković, Nina | |
dc.creator | Radibratović, Milica | |
dc.creator | Mihailović-Vesić, Jelena | |
dc.creator | Vasović, Tamara | |
dc.creator | Nikolić, Milan | |
dc.creator | Milčić, Miloš K. | |
dc.creator | Stanić-Vučinić, Dragana | |
dc.creator | Ćirković-Veličković, Tanja | |
dc.date.accessioned | 2019-07-01T09:26:06Z | |
dc.date.available | 2019-06-27 | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0308-8146 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/3186 | |
dc.description.abstract | In this study, we investigated structural aspects of covalent binding of food derived blue pigment phycocyanobilin (PCB) to bovine beta-lactoglobulin (BLG), major whey protein, by spectroscopic, electrophoretic, mass spectrometry and computational methods. At physiological pH (7.2), we found that covalent pigment binding via free cysteine residue is slow (k(a)=0.065 min(-1)), of moderate affinity (K-a=4x10(4) M-1), and stereo-selective. Binding also occurs at a broad pH range and under simulated gastrointestinal conditions. Adduct formation rises with pH, and in concentrated urea (k(a)=0.101 min(-1)). The BLG-PCB adduct has slightly altered secondary and tertiary protein structure, and bound PCB has higher fluorescence and more stretched conformation than free chromophore. Combination of steered molecular dynamic for disulfide exchange, non-covalent and covalent docking, favours Cys119 residue in protein calyx as target for covalent BLG-PCB adduct formation. Our results suggest that this adduct can serve as delivery system of bioactive PCB. | en |
dc.publisher | Elsevier Sci Ltd, Oxford | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172024/RS// | |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/256716/EU// | |
dc.rights | embargoedAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Food Chemistry | |
dc.subject | beta-lactoglobulin | en |
dc.subject | Phycocyanobilin | en |
dc.subject | Phycocyanin | en |
dc.subject | Covalent | en |
dc.subject | Binding | en |
dc.subject | Spirulina | en |
dc.subject | Fluorescence | en |
dc.subject | Molecular docking | en |
dc.title | Covalent binding of food-derived blue pigment phycocyanobilin to bovine beta-lactoglobulin under physiological conditions | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Радибратовић, Милица; Савковић, Нина; Васовић, Тамара; Николић, Милан; Станић-Вучинић, Драгана; Ћирковић-Величковић, Тања; Минић, Симеон Л.; Радомировић, Мирјана Ж.; Михаиловић-Весић, Јелена; Милчић, Милош К.; | |
dc.citation.volume | 269 | |
dc.citation.spage | 43 | |
dc.citation.epage | 52 | |
dc.identifier.wos | 000441142100006 | |
dc.identifier.doi | 10.1016/j.foodchem.2018.06.138 | |
dc.citation.other | 269: 43-52 | |
dc.citation.rank | aM21 | |
dc.identifier.pmid | 30100456 | |
dc.description.other | This is peer-reviewed version of the following article: Minic, S.; Radomirovic, M.; Savkovic, N.; Radibratovic, M.; Mihailovic, J.; Vasovic, T.; Nikolic, M.; Milcic, M.; Stanic-Vucinic, D.; Cirkovic Velickovic, T. Covalent Binding of Food-Derived Blue Pigment Phycocyanobilin to Bovine β-Lactoglobulin under Physiological Conditions. Food Chemistry 2018, 269, 43–52.[https://doi.org/10.1016/j.foodchem.2018.06.138] | |
dc.description.other | Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3187] | |
dc.type.version | acceptedVersion | en |
dc.identifier.scopus | 2-s2.0-85049319228 | |
dc.identifier.fulltext | https://cherry.chem.bg.ac.rs/bitstream/id/7837/Covalent_binding_of_acc_2018.pdf | |