Centaurea ragusina L., an endemic Croatian plant species, revealed a good cytotoxic activity of aqueous extracts (AE) on human bladder (T24) and human glioblastoma (A1235) cancer cell lines. The chemical constituents were tentatively identified using high performance liquid chromatography HPLC-DAD/ ESI-TOF-MS in negative ionization mode. The main compounds of herba extract were sesquiterpene lactones: solstitialin A 3,13-diacetate and epoxyrepdiolide; organic acid: quinic acid. The main compounds of flower extract were organic acids: quinic acid, citric acid, and malic acid; sesquiterpene lactone: cynaropicrin; phenolic compounds: chlorogenic acid and phenylpropanoid: syringin. The AE of C. ragusina were investigated for correlation of their effects on human bladder (T24) and human glioblastoma (A1235) cancer cell lines using the MTT assay. Although both extracts showed significant dose-and time-dependent cytotoxic activity against both cancer cell lines, the flower extract exhibited s...lightly higher activity. In order to determine type of cell death induced by treatment, cell lines were exposed subsequently to a treatment with both flower and herba AE. The majority of the cells died by induced apoptosis treatment. Flower AE (26.25%), compared to a leaf AE (22.15%) showed slightly higher percentage of an apoptosis in T24 cells, when compared to a non-treated cells (0.04%).
Chemistry and Biodiversity, 2017, 14, 9, e1700099-
Publisher:
Wiley-V C H Verlag Gmbh, Weinheim
Funding / projects:
Croatian Science Foundation [IP-2014-09-6897]
Note:
This is the peer-reviewed version of the following article: Radan, M.; Carev, I.; Tešević, V.; Politeo, O.; Čulić, V. Č. Qualitative HPLC-DAD/ESI-TOF-MS Analysis, Cytotoxic, and Apoptotic Effects of Croatian Endemic Centaurea Ragusina L. Aqueous Extracts. Chemistry and Biodiversity 2017, 14 (9). https://doi.org/10.1002/cbdv.201700099
TY - JOUR
AU - Radan, Mila
AU - Carev, Ivana
AU - Tešević, Vele
AU - Politeo, Olivera
AU - Čulić, Vedrana Čikeš
PY - 2017
UR - https://cherry.chem.bg.ac.rs/handle/123456789/3245
AB - Centaurea ragusina L., an endemic Croatian plant species, revealed a good cytotoxic activity of aqueous extracts (AE) on human bladder (T24) and human glioblastoma (A1235) cancer cell lines. The chemical constituents were tentatively identified using high performance liquid chromatography HPLC-DAD/ ESI-TOF-MS in negative ionization mode. The main compounds of herba extract were sesquiterpene lactones: solstitialin A 3,13-diacetate and epoxyrepdiolide; organic acid: quinic acid. The main compounds of flower extract were organic acids: quinic acid, citric acid, and malic acid; sesquiterpene lactone: cynaropicrin; phenolic compounds: chlorogenic acid and phenylpropanoid: syringin. The AE of C. ragusina were investigated for correlation of their effects on human bladder (T24) and human glioblastoma (A1235) cancer cell lines using the MTT assay. Although both extracts showed significant dose-and time-dependent cytotoxic activity against both cancer cell lines, the flower extract exhibited slightly higher activity. In order to determine type of cell death induced by treatment, cell lines were exposed subsequently to a treatment with both flower and herba AE. The majority of the cells died by induced apoptosis treatment. Flower AE (26.25%), compared to a leaf AE (22.15%) showed slightly higher percentage of an apoptosis in T24 cells, when compared to a non-treated cells (0.04%).
PB - Wiley-V C H Verlag Gmbh, Weinheim
T2 - Chemistry and Biodiversity
T1 - Qualitative HPLC-DAD/ESI-TOF-MS Analysis, Cytotoxic, and Apoptotic Effects of Croatian Endemic Centaurea ragusina L. Aqueous Extracts
VL - 14
IS - 9
SP - e1700099
DO - 10.1002/cbdv.201700099
ER -
@article{
author = "Radan, Mila and Carev, Ivana and Tešević, Vele and Politeo, Olivera and Čulić, Vedrana Čikeš",
year = "2017",
abstract = "Centaurea ragusina L., an endemic Croatian plant species, revealed a good cytotoxic activity of aqueous extracts (AE) on human bladder (T24) and human glioblastoma (A1235) cancer cell lines. The chemical constituents were tentatively identified using high performance liquid chromatography HPLC-DAD/ ESI-TOF-MS in negative ionization mode. The main compounds of herba extract were sesquiterpene lactones: solstitialin A 3,13-diacetate and epoxyrepdiolide; organic acid: quinic acid. The main compounds of flower extract were organic acids: quinic acid, citric acid, and malic acid; sesquiterpene lactone: cynaropicrin; phenolic compounds: chlorogenic acid and phenylpropanoid: syringin. The AE of C. ragusina were investigated for correlation of their effects on human bladder (T24) and human glioblastoma (A1235) cancer cell lines using the MTT assay. Although both extracts showed significant dose-and time-dependent cytotoxic activity against both cancer cell lines, the flower extract exhibited slightly higher activity. In order to determine type of cell death induced by treatment, cell lines were exposed subsequently to a treatment with both flower and herba AE. The majority of the cells died by induced apoptosis treatment. Flower AE (26.25%), compared to a leaf AE (22.15%) showed slightly higher percentage of an apoptosis in T24 cells, when compared to a non-treated cells (0.04%).",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry and Biodiversity",
title = "Qualitative HPLC-DAD/ESI-TOF-MS Analysis, Cytotoxic, and Apoptotic Effects of Croatian Endemic Centaurea ragusina L. Aqueous Extracts",
volume = "14",
number = "9",
pages = "e1700099",
doi = "10.1002/cbdv.201700099"
}
Radan, M., Carev, I., Tešević, V., Politeo, O.,& Čulić, V. Č.. (2017). Qualitative HPLC-DAD/ESI-TOF-MS Analysis, Cytotoxic, and Apoptotic Effects of Croatian Endemic Centaurea ragusina L. Aqueous Extracts. in Chemistry and Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 14(9), e1700099.
https://doi.org/10.1002/cbdv.201700099
Radan M, Carev I, Tešević V, Politeo O, Čulić VČ. Qualitative HPLC-DAD/ESI-TOF-MS Analysis, Cytotoxic, and Apoptotic Effects of Croatian Endemic Centaurea ragusina L. Aqueous Extracts. in Chemistry and Biodiversity. 2017;14(9):e1700099.
doi:10.1002/cbdv.201700099 .
Radan, Mila, Carev, Ivana, Tešević, Vele, Politeo, Olivera, Čulić, Vedrana Čikeš, "Qualitative HPLC-DAD/ESI-TOF-MS Analysis, Cytotoxic, and Apoptotic Effects of Croatian Endemic Centaurea ragusina L. Aqueous Extracts" in Chemistry and Biodiversity, 14, no. 9 (2017):e1700099,
https://doi.org/10.1002/cbdv.201700099 . .
