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Accumulation of U, Th, Pb, V, Rb, and Ag in wild Mushrooms macrolepiota procera (Scop.) Singer from Goc, Serbia

Authorized Users Only
2019
Authors
Vukojević, Vesna
Đurđić, Slađana Z.
Mutić, Jelena
Article (Published version)
Metadata
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Abstract
Cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium can be used in lactobionic acid production, biosensor for lactose, biofuel cells, lignocellulose degradation, and wound-healing applications. To make it a better biocatalyst, CDH with higher activity in an immobilized form is desirable. For this purpose, CDH was expressed for the first time on the surface of S. cerevisiae EBY100 cells in an active form as a triple mutant tmCDH (D20N, A64T, V592M) and evolved further for higher activity using resazurin-based fluorescent assay. In order to decrease blank reaction of resazurin with yeast cells and to have linear correlation between enzyme activity on the cell surface and fluorescence signal, the assay was optimized with respect to resazurin concentration (0.1 mM), substrate concentration (10mMlactose and 0.08mMcellobiose), and pH (6.0). Using optimized assay an error prone PCR gene library of tmCDH was screened. Two mutants with 5 (H5) and 7 mutations (H9) were found having t...wo times higher activity than the parent tmCDH enzyme that already had improved activity compared to wild type CDH whose activity could not be detected on the surface of yeast cells.

Keywords:
Actinides / Bioindicator / Mycoremediation / Rubidium / Vanadium
Source:
Environmental Science and Pollution Research, 2019, 26, 13, 13147-13158
Funding / projects:
  • Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-172030)
  • FoodEnTwin-Twinning of research activities for the frontier research in the fields of food, nutrition and environmental omics (EU-810752)
Note:
  • Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3284

DOI: 10.1007/s11356-019-04723-z

ISSN: 0944-1344

WoS: 000465460000045

Scopus: 2-s2.0-85063215632
[ Google Scholar ]
10
8
URI
https://cherry.chem.bg.ac.rs/handle/123456789/3283
Collections
  • Publikacije
Institution/Community
Hemijski fakultet
TY  - JOUR
AU  - Vukojević, Vesna
AU  - Đurđić, Slađana Z.
AU  - Mutić, Jelena
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3283
AB  - Cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium can be used in lactobionic acid production, biosensor for lactose, biofuel cells, lignocellulose degradation, and wound-healing applications. To make it a better biocatalyst, CDH with higher activity in an immobilized form is desirable. For this purpose, CDH was expressed for the first time on the surface of S. cerevisiae EBY100 cells in an active form as a triple mutant tmCDH (D20N, A64T, V592M) and evolved further for higher activity using resazurin-based fluorescent assay. In order to decrease blank reaction of resazurin with yeast cells and to have linear correlation between enzyme activity on the cell surface and fluorescence signal, the assay was optimized with respect to resazurin concentration (0.1 mM), substrate concentration (10mMlactose and 0.08mMcellobiose), and pH (6.0). Using optimized assay an error prone PCR gene library of tmCDH was screened. Two mutants with 5 (H5) and 7 mutations (H9) were found having two times higher activity than the parent tmCDH enzyme that already had improved activity compared to wild type CDH whose activity could not be detected on the surface of yeast cells.
T2  - Environmental Science and Pollution Research
T1  - Accumulation of U, Th, Pb, V, Rb, and Ag in wild Mushrooms macrolepiota procera (Scop.) Singer from Goc, Serbia
VL  - 26
IS  - 13
SP  - 13147
EP  - 13158
DO  - 10.1007/s11356-019-04723-z
ER  - 
@article{
author = "Vukojević, Vesna and Đurđić, Slađana Z. and Mutić, Jelena",
year = "2019",
abstract = "Cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium can be used in lactobionic acid production, biosensor for lactose, biofuel cells, lignocellulose degradation, and wound-healing applications. To make it a better biocatalyst, CDH with higher activity in an immobilized form is desirable. For this purpose, CDH was expressed for the first time on the surface of S. cerevisiae EBY100 cells in an active form as a triple mutant tmCDH (D20N, A64T, V592M) and evolved further for higher activity using resazurin-based fluorescent assay. In order to decrease blank reaction of resazurin with yeast cells and to have linear correlation between enzyme activity on the cell surface and fluorescence signal, the assay was optimized with respect to resazurin concentration (0.1 mM), substrate concentration (10mMlactose and 0.08mMcellobiose), and pH (6.0). Using optimized assay an error prone PCR gene library of tmCDH was screened. Two mutants with 5 (H5) and 7 mutations (H9) were found having two times higher activity than the parent tmCDH enzyme that already had improved activity compared to wild type CDH whose activity could not be detected on the surface of yeast cells.",
journal = "Environmental Science and Pollution Research",
title = "Accumulation of U, Th, Pb, V, Rb, and Ag in wild Mushrooms macrolepiota procera (Scop.) Singer from Goc, Serbia",
volume = "26",
number = "13",
pages = "13147-13158",
doi = "10.1007/s11356-019-04723-z"
}
Vukojević, V., Đurđić, S. Z.,& Mutić, J.. (2019). Accumulation of U, Th, Pb, V, Rb, and Ag in wild Mushrooms macrolepiota procera (Scop.) Singer from Goc, Serbia. in Environmental Science and Pollution Research, 26(13), 13147-13158.
https://doi.org/10.1007/s11356-019-04723-z
Vukojević V, Đurđić SZ, Mutić J. Accumulation of U, Th, Pb, V, Rb, and Ag in wild Mushrooms macrolepiota procera (Scop.) Singer from Goc, Serbia. in Environmental Science and Pollution Research. 2019;26(13):13147-13158.
doi:10.1007/s11356-019-04723-z .
Vukojević, Vesna, Đurđić, Slađana Z., Mutić, Jelena, "Accumulation of U, Th, Pb, V, Rb, and Ag in wild Mushrooms macrolepiota procera (Scop.) Singer from Goc, Serbia" in Environmental Science and Pollution Research, 26, no. 13 (2019):13147-13158,
https://doi.org/10.1007/s11356-019-04723-z . .

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