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dc.creatorSimić, Stefan
dc.creatorJeremić, Sanja
dc.creatorĐokić, Lidija
dc.creatorBožić, Nataša
dc.creatorVujčić, Zoran
dc.creatorLončar, Nikola L.
dc.creatorSenthamaraikannan, Ramsankar
dc.creatorBabu, Ramesh P.
dc.creatorOpsenica, Igor
dc.creatorNikodinović-Runić, Jasmina
dc.date.accessioned2019-09-02T15:31:57Z
dc.date.available2019-09-02T15:31:57Z
dc.date.issued2020
dc.identifier.issn0141-0229
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/3356
dc.description.abstractBiocatalytic oxidations mediated by laccases are gaining importance due to their versatility and beneficial environmental effects. In this study, the oxidation of 1,4-dihydropyridines has been performed using three different types of bacterial laccase-based catalysts: purified laccase from Bacillus licheniformis ATCC 9945a (BliLacc), Escherichia coli whole cells expressing this laccase, and bacterial nanocellulose (BNC) supported BliLacc catalysts. The catalysts based on bacterial laccase were compared to the commercially available Trametes versicolor laccase (TvLacc). The oxidation product of 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate was obtained within 7–24 h with good yields (70–99%) with all three biocatalysts. The substrate scope was examined with five additional 1,4-dihydropyridines, one of which was oxidized in high yield. Whole-cell biocatalyst was stable when stored for up to 1-month at 4 °C. In addition, evidence has been provided that multicopper oxidase CueO from the E. coli expression host contributed to the oxidation efficiency of the whole-cell biocatalyst. The immobilized whole-cell biocatalyst showed satisfactory activity and retained 37% of its original activity after three biotransformation cycles.
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173048/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172008/RS//
dc.rightsrestrictedAccess
dc.sourceEnzyme and Microbial Technology
dc.subject1,4-dihydropyridines
dc.subjectBacterial nanocellulose
dc.subjectImmobilization
dc.subjectLaccase
dc.subjectWhole-cell biocatalysis
dc.titleDevelopment of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines
dc.typearticle
dc.rights.licenseARR
dcterms.abstractОпсеница, Игор; Симић, Стефан; Божић, Наташа М.; Вујчић, Зоран; Лончар, Никола; Сентхамараиканнан, Рамсанкар; Никодиновић-Рунић, Јасмина; Јеремиц, Сања; Ђокић, Лидија; Бабу, Рамесх П.;
dc.citation.volume132
dc.identifier.wos000504531500015
dc.identifier.doi10.1016/j.enzmictec.2019.109411
dc.citation.rankM21~
dc.description.otherSupplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3357]
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85070994303


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