Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats
Само за регистроване кориснике
2019
Аутори
Kulaš, JelenaNinkov, Marina
Tucović, Dina
Popov-Aleksandrov, Aleksandra
Ukropina, Mirela
Cakić-Milošević, Maja
Mutić, Jelena
Kataranovski, Milena
Mikrov, Ivana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Objective The aim of this study is to investigate the effects of oral cadmium (Cd) ingestion on the pulmonary immune response. Methods Determination of Cd content in lungs and histopathological evaluation of the tissue was performed in rats following 30-day oral Cd administration (5 and 50 mg/L). Antioxidant enzyme defense (superoxide dismutase and catalase), cell infiltration, and production of tumor necrosis factor (TNF) and interferon (IFN)-γ, as well as the activity of myeloperoxidase (MPO), nitric oxide (NO), and various cytokines [interleukin (IL)-1β, IL-6, IL-10, and IL-17] were investigated. Results Cd caused tissue damage and cell infiltration in the lungs, and this damage was more pronounced at higher doses. Cd deposition resulted in lung inflammation characterized by a dose-dependent IL-1β increase in lung homogenates, increased TNF levels at both doses, and IL-6 stimulation at low doses with inhibition observed at higher doses. Cd exerted differential effects on lung leukoc...ytes isolated by enzyme digestion, and these effects were characterized by a lack of change in the production of reactive oxygen and nitrogen species, an inhibition of IL-1β and TNF, and stimulation of MPO and IFN-γ. The higher capacity of Cd-exposed lung cells to respond to the opportunistic pathogen Staphylococcus epidermidis was demonstrated in vitro. Conclusion The potential of ingested Cd to exert both proinflammatory and immunosuppressive effects on pulmonary tissue inflammation and immune reactivity highlights the complex immunomodulatory actions of this metal.
Кључне речи:
Cytokine response / Lungs / Oral cadmium administration / RatsИзвор:
Biomedical and environmental sciences : BES, 2019, 32, 7, 508-519Финансирање / пројекти:
- Имуномодулаторни ефекти ксенобиотика и биотичких фактора животне средине на популације мишоликих глодара (RS-MESTD-Basic Research (BR or ON)-173039)
DOI: 10.3967/bes2019.068
ISSN: 2214-0190
WoS: 000476473000004
Scopus: 2-s2.0-85071830951
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Kulaš, Jelena AU - Ninkov, Marina AU - Tucović, Dina AU - Popov-Aleksandrov, Aleksandra AU - Ukropina, Mirela AU - Cakić-Milošević, Maja AU - Mutić, Jelena AU - Kataranovski, Milena AU - Mikrov, Ivana PY - 2019 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3455 AB - Objective The aim of this study is to investigate the effects of oral cadmium (Cd) ingestion on the pulmonary immune response. Methods Determination of Cd content in lungs and histopathological evaluation of the tissue was performed in rats following 30-day oral Cd administration (5 and 50 mg/L). Antioxidant enzyme defense (superoxide dismutase and catalase), cell infiltration, and production of tumor necrosis factor (TNF) and interferon (IFN)-γ, as well as the activity of myeloperoxidase (MPO), nitric oxide (NO), and various cytokines [interleukin (IL)-1β, IL-6, IL-10, and IL-17] were investigated. Results Cd caused tissue damage and cell infiltration in the lungs, and this damage was more pronounced at higher doses. Cd deposition resulted in lung inflammation characterized by a dose-dependent IL-1β increase in lung homogenates, increased TNF levels at both doses, and IL-6 stimulation at low doses with inhibition observed at higher doses. Cd exerted differential effects on lung leukocytes isolated by enzyme digestion, and these effects were characterized by a lack of change in the production of reactive oxygen and nitrogen species, an inhibition of IL-1β and TNF, and stimulation of MPO and IFN-γ. The higher capacity of Cd-exposed lung cells to respond to the opportunistic pathogen Staphylococcus epidermidis was demonstrated in vitro. Conclusion The potential of ingested Cd to exert both proinflammatory and immunosuppressive effects on pulmonary tissue inflammation and immune reactivity highlights the complex immunomodulatory actions of this metal. T2 - Biomedical and environmental sciences : BES T1 - Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats VL - 32 IS - 7 SP - 508 EP - 519 DO - 10.3967/bes2019.068 ER -
@article{ author = "Kulaš, Jelena and Ninkov, Marina and Tucović, Dina and Popov-Aleksandrov, Aleksandra and Ukropina, Mirela and Cakić-Milošević, Maja and Mutić, Jelena and Kataranovski, Milena and Mikrov, Ivana", year = "2019", abstract = "Objective The aim of this study is to investigate the effects of oral cadmium (Cd) ingestion on the pulmonary immune response. Methods Determination of Cd content in lungs and histopathological evaluation of the tissue was performed in rats following 30-day oral Cd administration (5 and 50 mg/L). Antioxidant enzyme defense (superoxide dismutase and catalase), cell infiltration, and production of tumor necrosis factor (TNF) and interferon (IFN)-γ, as well as the activity of myeloperoxidase (MPO), nitric oxide (NO), and various cytokines [interleukin (IL)-1β, IL-6, IL-10, and IL-17] were investigated. Results Cd caused tissue damage and cell infiltration in the lungs, and this damage was more pronounced at higher doses. Cd deposition resulted in lung inflammation characterized by a dose-dependent IL-1β increase in lung homogenates, increased TNF levels at both doses, and IL-6 stimulation at low doses with inhibition observed at higher doses. Cd exerted differential effects on lung leukocytes isolated by enzyme digestion, and these effects were characterized by a lack of change in the production of reactive oxygen and nitrogen species, an inhibition of IL-1β and TNF, and stimulation of MPO and IFN-γ. The higher capacity of Cd-exposed lung cells to respond to the opportunistic pathogen Staphylococcus epidermidis was demonstrated in vitro. Conclusion The potential of ingested Cd to exert both proinflammatory and immunosuppressive effects on pulmonary tissue inflammation and immune reactivity highlights the complex immunomodulatory actions of this metal.", journal = "Biomedical and environmental sciences : BES", title = "Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats", volume = "32", number = "7", pages = "508-519", doi = "10.3967/bes2019.068" }
Kulaš, J., Ninkov, M., Tucović, D., Popov-Aleksandrov, A., Ukropina, M., Cakić-Milošević, M., Mutić, J., Kataranovski, M.,& Mikrov, I.. (2019). Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats. in Biomedical and environmental sciences : BES, 32(7), 508-519. https://doi.org/10.3967/bes2019.068
Kulaš J, Ninkov M, Tucović D, Popov-Aleksandrov A, Ukropina M, Cakić-Milošević M, Mutić J, Kataranovski M, Mikrov I. Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats. in Biomedical and environmental sciences : BES. 2019;32(7):508-519. doi:10.3967/bes2019.068 .
Kulaš, Jelena, Ninkov, Marina, Tucović, Dina, Popov-Aleksandrov, Aleksandra, Ukropina, Mirela, Cakić-Milošević, Maja, Mutić, Jelena, Kataranovski, Milena, Mikrov, Ivana, "Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats" in Biomedical and environmental sciences : BES, 32, no. 7 (2019):508-519, https://doi.org/10.3967/bes2019.068 . .