Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models
Authorized Users Only
2020
Authors
Đorđević, JelenaKolarević, Stoimir

Jovanović, Jovana
Kostić-Vuković, Jovana
Novaković, Irena T.

Jeremić, Marko

Sladić, Dušan

Vuković-Gačić, Branka

Article (Published version)

Metadata
Show full item recordAbstract
Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2). Results indicated that in the acellular model TBQ and its derivatives do not interact with plasmid pUC19. In the prokaryotic model, only TBQ exerted weak genotoxic potential and only at highly cytotoxic concentrations. In eukaryotic models, genotoxic potential was detected mainly at the highest... concentrations of the tested substances but the effect was lower in both cell lines in comparison with benzo[a]pyrene and etoposide which were used as positive controls. Weak genotoxic potential of tested compounds recommends them as good candidates for further testing in development of new antitumor agents. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
Keywords:
alkylamino and aralkylamino derivatives / comet assay / genotoxicity / plasmid relaxation / SOS/umuC / Tert-butylquinoneSource:
Drug and Chemical Toxicology, 2020, 43, 5Publisher:
- Taylor & Francis
Funding / projects:
- Interactions of natural products, their derivatives and coordination compounds with proteins and nucleic acids (RS-172055)
- Bioactive natural products as potential sources of new pharmaceuticals and food supplements (RS-172058)
DOI: 10.1080/01480545.2018.1514043
ISSN: 0148-0545
WoS: 000551592600008
Scopus: 2-s2.0-85053908360
Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Đorđević, Jelena AU - Kolarević, Stoimir AU - Jovanović, Jovana AU - Kostić-Vuković, Jovana AU - Novaković, Irena T. AU - Jeremić, Marko AU - Sladić, Dušan AU - Vuković-Gačić, Branka PY - 2020 UR - https://cherry.chem.bg.ac.rs/handle/123456789/347 AB - Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2). Results indicated that in the acellular model TBQ and its derivatives do not interact with plasmid pUC19. In the prokaryotic model, only TBQ exerted weak genotoxic potential and only at highly cytotoxic concentrations. In eukaryotic models, genotoxic potential was detected mainly at the highest concentrations of the tested substances but the effect was lower in both cell lines in comparison with benzo[a]pyrene and etoposide which were used as positive controls. Weak genotoxic potential of tested compounds recommends them as good candidates for further testing in development of new antitumor agents. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group. PB - Taylor & Francis T2 - Drug and Chemical Toxicology T1 - Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models VL - 43 IS - 5 DO - 10.1080/01480545.2018.1514043 ER -
@article{ author = "Đorđević, Jelena and Kolarević, Stoimir and Jovanović, Jovana and Kostić-Vuković, Jovana and Novaković, Irena T. and Jeremić, Marko and Sladić, Dušan and Vuković-Gačić, Branka", year = "2020", abstract = "Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2). Results indicated that in the acellular model TBQ and its derivatives do not interact with plasmid pUC19. In the prokaryotic model, only TBQ exerted weak genotoxic potential and only at highly cytotoxic concentrations. In eukaryotic models, genotoxic potential was detected mainly at the highest concentrations of the tested substances but the effect was lower in both cell lines in comparison with benzo[a]pyrene and etoposide which were used as positive controls. Weak genotoxic potential of tested compounds recommends them as good candidates for further testing in development of new antitumor agents. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.", publisher = "Taylor & Francis", journal = "Drug and Chemical Toxicology", title = "Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models", volume = "43", number = "5", doi = "10.1080/01480545.2018.1514043" }
Đorđević, J., Kolarević, S., Jovanović, J., Kostić-Vuković, J., Novaković, I. T., Jeremić, M., Sladić, D.,& Vuković-Gačić, B.. (2020). Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology Taylor & Francis., 43(5). https://doi.org/10.1080/01480545.2018.1514043
Đorđević J, Kolarević S, Jovanović J, Kostić-Vuković J, Novaković IT, Jeremić M, Sladić D, Vuković-Gačić B. Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology. 2020;43(5). doi:10.1080/01480545.2018.1514043 .
Đorđević, Jelena, Kolarević, Stoimir, Jovanović, Jovana, Kostić-Vuković, Jovana, Novaković, Irena T., Jeremić, Marko, Sladić, Dušan, Vuković-Gačić, Branka, "Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models" in Drug and Chemical Toxicology, 43, no. 5 (2020), https://doi.org/10.1080/01480545.2018.1514043 . .