Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium
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2019
Authors
Gojgić-Cvijović, Gordana D.
Jakovljević, Dragica M.

Lončarević, Branka D.
Todorović, Nevena M.
Pergal, Marija V.

Ćirić, J.
Loos, K.
Beškoski, Vladimir

Vrvić, Miroslav M.

Article (Published version)

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The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100–140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 × 106 Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium ...with 200 g/L sucrose. © 2018 Elsevier B.V.
Keywords:
Levan / Molasses / Molecular weightSource:
International Journal of Biological Macromolecules, 2019, 121, 142-151Funding / projects:
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-172053)
- Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-172062)
Note:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/2943
- Peer reviewed manuscript: http://cherry.chem.bg.ac.rs/handle/123456789/2799
DOI: 10.1016/j.ijbiomac.2018.10.019
ISSN: 0141-8130
WoS: 000452346100018
Scopus: 2-s2.0-85054437248
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Gojgić-Cvijović, Gordana D. AU - Jakovljević, Dragica M. AU - Lončarević, Branka D. AU - Todorović, Nevena M. AU - Pergal, Marija V. AU - Ćirić, J. AU - Loos, K. AU - Beškoski, Vladimir AU - Vrvić, Miroslav M. PY - 2019 UR - https://cherry.chem.bg.ac.rs/handle/123456789/350 AB - The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100–140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 × 106 Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose. © 2018 Elsevier B.V. T2 - International Journal of Biological Macromolecules T1 - Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium VL - 121 SP - 142 EP - 151 DO - 10.1016/j.ijbiomac.2018.10.019 ER -
@article{ author = "Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Lončarević, Branka D. and Todorović, Nevena M. and Pergal, Marija V. and Ćirić, J. and Loos, K. and Beškoski, Vladimir and Vrvić, Miroslav M.", year = "2019", abstract = "The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100–140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 × 106 Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose. © 2018 Elsevier B.V.", journal = "International Journal of Biological Macromolecules", title = "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium", volume = "121", pages = "142-151", doi = "10.1016/j.ijbiomac.2018.10.019" }
Gojgić-Cvijović, G. D., Jakovljević, D. M., Lončarević, B. D., Todorović, N. M., Pergal, M. V., Ćirić, J., Loos, K., Beškoski, V.,& Vrvić, M. M.. (2019). Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules, 121, 142-151. https://doi.org/10.1016/j.ijbiomac.2018.10.019
Gojgić-Cvijović GD, Jakovljević DM, Lončarević BD, Todorović NM, Pergal MV, Ćirić J, Loos K, Beškoski V, Vrvić MM. Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules. 2019;121:142-151. doi:10.1016/j.ijbiomac.2018.10.019 .
Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Lončarević, Branka D., Todorović, Nevena M., Pergal, Marija V., Ćirić, J., Loos, K., Beškoski, Vladimir, Vrvić, Miroslav M., "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium" in International Journal of Biological Macromolecules, 121 (2019):142-151, https://doi.org/10.1016/j.ijbiomac.2018.10.019 . .