Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant
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2019
Authors
Kostić, Aleksandar Ž.Milinčić, Danijel D.
Gašić, Uroš M.
Nedić, Nebojša
Stanojević, Slađana P.
Tešić, Živoslav Lj.
Pešić, Mirjana B.
Article (Published version)
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The aim of this work was to determine phenolic profile and antioxidant properties of methanolic (MEP) and ethanolic (EEP) extracts of monofloral bee-collected sunflower pollen from Serbia. In total, 37 different polyphenols (19 flavonol glycosides and 18 other polyphenols) were identified by UHPLC/MS-MS Orbitrap analysis. The different phenolic profile of MEP and EEP was observed with flavonols as most abundant, 94.1% (MEP) and 93.4% (EEP), out of which flavonoid glycosides represented 73.2% (MEP) and 66.1% (EEP). The higher amount of total phenolics was recorded in EEP (200.58 mg/kg DW) than in MEP (244.44 mg/kg DW). The quercetin 3-O-galactoside was the major compound in both extracts, 112.86 mg/kg in MEP and 128.64 mg/kg in EEP. The presence of coumaroylated and acetylated flavonoid glycosides were registered, among which the quercetin 3-O-(6“-acetyl)hexoside was recored in the significant amount in MEP (111.10 mg Q3G/kg DW), whereas in EEP the content of coumaroylated kaempferol gl...ycosides (137.95 mg Q3G/kg DW) was significantly higher. The ferric reducing power and ABTS radical scavenging activity assays revealed that pollen extracts possessed significant antioxidant activity. The monofloral bee-collected pollen of Helianthus annuus L. can be used as good food supplement with high flavonoids content and antioxidant activity.
Keywords:
Antioxidant activity / Ethanolic extract / Methanolic extract / Monofloral sunflower pollen / Phenolic profileSource:
LWT, 2019, 112Publisher:
- Academic Press
Funding / projects:
- Utilization of plant sources of protein, dietary fiber and antioxidants in food production (RS-MESTD-Technological Development (TD or TR)-31069)
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-MESTD-Basic Research (BR or ON)-172017)
- Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46008)
Note:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3714
DOI: 10.1016/j.lwt.2019.06.011
ISSN: 0023-6438
WoS: 000476965700023
Scopus: 2-s2.0-85066933155
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Kostić, Aleksandar Ž. AU - Milinčić, Danijel D. AU - Gašić, Uroš M. AU - Nedić, Nebojša AU - Stanojević, Slađana P. AU - Tešić, Živoslav Lj. AU - Pešić, Mirjana B. PY - 2019 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3713 AB - The aim of this work was to determine phenolic profile and antioxidant properties of methanolic (MEP) and ethanolic (EEP) extracts of monofloral bee-collected sunflower pollen from Serbia. In total, 37 different polyphenols (19 flavonol glycosides and 18 other polyphenols) were identified by UHPLC/MS-MS Orbitrap analysis. The different phenolic profile of MEP and EEP was observed with flavonols as most abundant, 94.1% (MEP) and 93.4% (EEP), out of which flavonoid glycosides represented 73.2% (MEP) and 66.1% (EEP). The higher amount of total phenolics was recorded in EEP (200.58 mg/kg DW) than in MEP (244.44 mg/kg DW). The quercetin 3-O-galactoside was the major compound in both extracts, 112.86 mg/kg in MEP and 128.64 mg/kg in EEP. The presence of coumaroylated and acetylated flavonoid glycosides were registered, among which the quercetin 3-O-(6“-acetyl)hexoside was recored in the significant amount in MEP (111.10 mg Q3G/kg DW), whereas in EEP the content of coumaroylated kaempferol glycosides (137.95 mg Q3G/kg DW) was significantly higher. The ferric reducing power and ABTS radical scavenging activity assays revealed that pollen extracts possessed significant antioxidant activity. The monofloral bee-collected pollen of Helianthus annuus L. can be used as good food supplement with high flavonoids content and antioxidant activity. PB - Academic Press T2 - LWT T1 - Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant VL - 112 DO - 10.1016/j.lwt.2019.06.011 ER -
@article{ author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Gašić, Uroš M. and Nedić, Nebojša and Stanojević, Slađana P. and Tešić, Živoslav Lj. and Pešić, Mirjana B.", year = "2019", abstract = "The aim of this work was to determine phenolic profile and antioxidant properties of methanolic (MEP) and ethanolic (EEP) extracts of monofloral bee-collected sunflower pollen from Serbia. In total, 37 different polyphenols (19 flavonol glycosides and 18 other polyphenols) were identified by UHPLC/MS-MS Orbitrap analysis. The different phenolic profile of MEP and EEP was observed with flavonols as most abundant, 94.1% (MEP) and 93.4% (EEP), out of which flavonoid glycosides represented 73.2% (MEP) and 66.1% (EEP). The higher amount of total phenolics was recorded in EEP (200.58 mg/kg DW) than in MEP (244.44 mg/kg DW). The quercetin 3-O-galactoside was the major compound in both extracts, 112.86 mg/kg in MEP and 128.64 mg/kg in EEP. The presence of coumaroylated and acetylated flavonoid glycosides were registered, among which the quercetin 3-O-(6“-acetyl)hexoside was recored in the significant amount in MEP (111.10 mg Q3G/kg DW), whereas in EEP the content of coumaroylated kaempferol glycosides (137.95 mg Q3G/kg DW) was significantly higher. The ferric reducing power and ABTS radical scavenging activity assays revealed that pollen extracts possessed significant antioxidant activity. The monofloral bee-collected pollen of Helianthus annuus L. can be used as good food supplement with high flavonoids content and antioxidant activity.", publisher = "Academic Press", journal = "LWT", title = "Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant", volume = "112", doi = "10.1016/j.lwt.2019.06.011" }
Kostić, A. Ž., Milinčić, D. D., Gašić, U. M., Nedić, N., Stanojević, S. P., Tešić, Ž. Lj.,& Pešić, M. B.. (2019). Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant. in LWT Academic Press., 112. https://doi.org/10.1016/j.lwt.2019.06.011
Kostić AŽ, Milinčić DD, Gašić UM, Nedić N, Stanojević SP, Tešić ŽL, Pešić MB. Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant. in LWT. 2019;112. doi:10.1016/j.lwt.2019.06.011 .
Kostić, Aleksandar Ž., Milinčić, Danijel D., Gašić, Uroš M., Nedić, Nebojša, Stanojević, Slađana P., Tešić, Živoslav Lj., Pešić, Mirjana B., "Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant" in LWT, 112 (2019), https://doi.org/10.1016/j.lwt.2019.06.011 . .