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dc.creatorGrujić, Marica
dc.creatorDojnov, Biljana
dc.creatorPotočnik, Ivana
dc.creatorAtanasova, Lea
dc.creatorDuduk, Bojan
dc.creatorSrebotnik, Ewald
dc.creatorDruzhinina, Irirna S.
dc.creatorKubicek, Christian P.
dc.creatorVujčić, Zoran
dc.date.accessioned2020-01-13T10:35:46Z
dc.date.available2020-01-13T10:35:46Z
dc.date.issued2019
dc.identifier.issn0959-3993
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/3768
dc.description.abstractLignocellulosic plant biomass is the world’s most abundant carbon source and has consequently attracted attention as a renewable resource for production of biofuels and commodity chemicals that could replace fossil resources. Due to its recalcitrant nature, it must be pretreated by chemical, physical or biological means prior to hydrolysis, introducing additional costs. In this paper, we tested the hypothesis that fungi which thrive on lignocellulosic material (straw, bark or soil) would be efficient in degrading untreated lignocellulose. Wheat straw was used as a model. We developed a fast and simple screening method for cellulase producers and tested one hundred Trichoderma strains isolated from wheat straw. The most potent strain—UB483FTG2/ TUCIM 4455, was isolated from substrate used for mushroom cultivation and was identified as T. guizhouense. After optimization of growth medium, high cellulase activity was already achieved after 72 h of fermentation on raw wheat straw, while the model cellulase overproducing strain T. reesei QM 9414 took 170 h and reached only 45% of the cellulase activity secreted by T. guizhouense. Maximum production levels were 1.1 U/mL (measured with CMC as cellulase substrate) and 0.7 U/mL (β-glucosidase assay). The T. guizhouense cellulase cocktail hydrolyzed raw wheat straw within 35 h. Our study shows that screening for fungi that successfully compete for special substrates in nature will lead to the isolation of strains with qualitatively and quantitatively superior enzymes needed for their digestion which could be used for industrial purposes.
dc.publisherSpringer Link
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS//
dc.relationAustrian Science Fund (FWF) Project Number P 25613 B20 to ISD
dc.rightsrestrictedAccess
dc.sourceWorld Journal of Microbiology and Biotechnology
dc.subjectCellulase enzyme complex
dc.subjectCellulases
dc.subjectTrichoderma
dc.subjectTrichoderma guizhouense
dc.subjectTrichoderma reesei
dc.subjectWheat straw
dc.titleSuperior cellulolytic activity of Trichoderma guizhouense on raw wheat straw
dc.typearticle
dc.rights.licenseARR
dcterms.abstractДрузхинина, Ирирна С.; Среботник, Еwалд; Дудук, Бојан; Aтанасова, Леа; Поточник, Ивана; Дојнов, Биљана; Кубицек, Цхристиан П.; Грујић, Марица; Вујчић, Зоран;
dc.citation.volume35
dc.citation.issue12
dc.identifier.wos000517949400001
dc.identifier.doi10.1007/s11274-019-2774-y
dc.citation.rankM22~
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85075668082


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