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dc.creatorUzelac, Tamara N.
dc.creatorNikolić-Kokić, Aleksandra
dc.creatorSpasić, Snežana
dc.creatorMačvanin, Mirjana T.
dc.creatorNikolić, Milan
dc.creatorMandić, Ljuba M.
dc.creatorJovanović, Vesna B.
dc.date.accessioned2020-02-25T16:37:41Z
dc.date.available2020-08-07
dc.date.issued2019
dc.identifier.issn0009-2797
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/3865
dc.description.abstractAntipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.sr
dc.language.isoensr
dc.publisherElseviersr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173014/RS//sr
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/810752/EU//sr
dc.rightsembargoedAccesssr
dc.sourceChemico-Biological Interactionssr
dc.subjectalbuminsr
dc.subjectclozapinesr
dc.subjectziprasidonesr
dc.subjectthiolssr
dc.titleOpposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids contentsr
dc.typearticlesr
dc.rights.licenseBY-NC-NDsr
dcterms.abstractМандић, Љуба М.; Јовановић, Весна Б.; Узелац, Тамара Н.; Николић-Кокић, Aлександра; Спасић, Снежана; Мачванин, Мирјана Т.; Николић, Милан;
dc.citation.volume311
dc.citation.issue108787
dc.identifier.wos000487813500020
dc.identifier.doi10.1016/j.cbi.2019.108787
dc.citation.rankM21~
dc.description.otherSupplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3335]
dc.description.otherThis is the peer-reviewed version of the following article: Uzelac, T. N.; Nikolić-Kokić, A. L.; Spasić, S. D.; Mačvanin, M. T.; Nikolić, M. R.; Mandić, L. M.; Jovanović, V. B. Opposite Clozapine and Ziprasidone Effects on the Reactivity of Plasma Albumin SH-Group Are the Consequence of Their Different Binding Properties Dependent on Protein Fatty Acids Content. Chemico-Biological Interactions 2019, 311. [https://doi.org/10.1016/j.cbi.2019.108787]
dc.type.versionacceptedVersionsr
dc.identifier.scopus2-s2.0-85070497802
dc.identifier.fulltexthttp://cherry.chem.bg.ac.rs/bitstream/id/16750/Opposite_clozapine_and_acc_2019.pdf


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