Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins
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1999
Authors
Ćirković-Veličković, TanjaBukilica, MN
Gavrović-Jankulović, Marija
Vujčić, Zoran
Petrović, S
Jankov, Ratko M.
Article (Published version)
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Background: Orchard grass (Dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. Chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of Lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins. Methods: Physicochemical characterization of derivatives was done by gel chromatography, SDS-PAGE, and isoelectric focusing. To examine the IgE-binding properties of these derivatives, we carried out immunoblotting. To examine the ability of derivatives to induce IgG production, we immunized rabbits. Skin prick testing with the allergoids was performed on 15 individuals allergic to orchard grass pollens and on two healthy subjects. Results: II was shown that the modified proteins retain their original molecular weights, but change pi to more acidic va...lues. In the case of allergoids, a strong reduction in IgE binding was found. Immunization of rabbits with allergoids showed that the derivatives retain the ability to induce IgG production, and that the antisera obtained in such a way react to native (unmodified) extract. The ability of derivatives to induce allergic reaction was significantly reduced. The patients (86.6%) included in our study exhibited less than 50% of native extract response. Among them, 53.3% had no response to one or both allergoids. Conclusions: These modification procedures yield allergoids with a reduced allergenic activity and preserved immunogenic potential suitable for use in immunotherapy.
Keywords:
allergoid / chemical modification / Dactylis glomerata / maleic anhydride / orchard grass / skin prick test / succinic anhydrideSource:
Allergy, 1999, 54, 2, 128-134Publisher:
- Munksgaard Int Publ Ltd, Copenhagen
DOI: 10.1034/j.1398-9995.1999.00778.x
ISSN: 0105-4538
PubMed: 10221435
WoS: 000079413400005
Scopus: 2-s2.0-0000670417
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Ćirković-Veličković, Tanja AU - Bukilica, MN AU - Gavrović-Jankulović, Marija AU - Vujčić, Zoran AU - Petrović, S AU - Jankov, Ratko M. PY - 1999 UR - https://cherry.chem.bg.ac.rs/handle/123456789/411 AB - Background: Orchard grass (Dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. Chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of Lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins. Methods: Physicochemical characterization of derivatives was done by gel chromatography, SDS-PAGE, and isoelectric focusing. To examine the IgE-binding properties of these derivatives, we carried out immunoblotting. To examine the ability of derivatives to induce IgG production, we immunized rabbits. Skin prick testing with the allergoids was performed on 15 individuals allergic to orchard grass pollens and on two healthy subjects. Results: II was shown that the modified proteins retain their original molecular weights, but change pi to more acidic values. In the case of allergoids, a strong reduction in IgE binding was found. Immunization of rabbits with allergoids showed that the derivatives retain the ability to induce IgG production, and that the antisera obtained in such a way react to native (unmodified) extract. The ability of derivatives to induce allergic reaction was significantly reduced. The patients (86.6%) included in our study exhibited less than 50% of native extract response. Among them, 53.3% had no response to one or both allergoids. Conclusions: These modification procedures yield allergoids with a reduced allergenic activity and preserved immunogenic potential suitable for use in immunotherapy. PB - Munksgaard Int Publ Ltd, Copenhagen T2 - Allergy T1 - Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins VL - 54 IS - 2 SP - 128 EP - 134 DO - 10.1034/j.1398-9995.1999.00778.x ER -
@article{ author = "Ćirković-Veličković, Tanja and Bukilica, MN and Gavrović-Jankulović, Marija and Vujčić, Zoran and Petrović, S and Jankov, Ratko M.", year = "1999", abstract = "Background: Orchard grass (Dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. Chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of Lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins. Methods: Physicochemical characterization of derivatives was done by gel chromatography, SDS-PAGE, and isoelectric focusing. To examine the IgE-binding properties of these derivatives, we carried out immunoblotting. To examine the ability of derivatives to induce IgG production, we immunized rabbits. Skin prick testing with the allergoids was performed on 15 individuals allergic to orchard grass pollens and on two healthy subjects. Results: II was shown that the modified proteins retain their original molecular weights, but change pi to more acidic values. In the case of allergoids, a strong reduction in IgE binding was found. Immunization of rabbits with allergoids showed that the derivatives retain the ability to induce IgG production, and that the antisera obtained in such a way react to native (unmodified) extract. The ability of derivatives to induce allergic reaction was significantly reduced. The patients (86.6%) included in our study exhibited less than 50% of native extract response. Among them, 53.3% had no response to one or both allergoids. Conclusions: These modification procedures yield allergoids with a reduced allergenic activity and preserved immunogenic potential suitable for use in immunotherapy.", publisher = "Munksgaard Int Publ Ltd, Copenhagen", journal = "Allergy", title = "Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins", volume = "54", number = "2", pages = "128-134", doi = "10.1034/j.1398-9995.1999.00778.x" }
Ćirković-Veličković, T., Bukilica, M., Gavrović-Jankulović, M., Vujčić, Z., Petrović, S.,& Jankov, R. M.. (1999). Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins. in Allergy Munksgaard Int Publ Ltd, Copenhagen., 54(2), 128-134. https://doi.org/10.1034/j.1398-9995.1999.00778.x
Ćirković-Veličković T, Bukilica M, Gavrović-Jankulović M, Vujčić Z, Petrović S, Jankov RM. Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins. in Allergy. 1999;54(2):128-134. doi:10.1034/j.1398-9995.1999.00778.x .
Ćirković-Veličković, Tanja, Bukilica, MN, Gavrović-Jankulović, Marija, Vujčić, Zoran, Petrović, S, Jankov, Ratko M., "Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins" in Allergy, 54, no. 2 (1999):128-134, https://doi.org/10.1034/j.1398-9995.1999.00778.x . .