Evolution of humic acids during ex situ bioremediation on a pilot level: The added value of the microbial activity
Authors
Žerađanin, AleksandraAvdalović, Jelena

Lješević, Marija

Tešić, Olivera
Miletić, Srđan B.

Vrvić, Miroslav M.

Beškoski, Vladimir

Article (Published version)
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Environmental pollution is a global problem, while bioremediation technology removes pollutants from the environment using microorganisms. This study was aimed at investigating how a bioremediation process affected soil humification. In soil polluted with petroleum and its derivatives that was submitted to bioremediation, besides the total petroleum hydrocarbons and the number of microorganisms, quantitative and qualitative changes of isolated humic acids were determined during the process. The bioremediation of 150 m3 of polluted soil lasted 150 days. The level of total petroleum hydrocarbons decreased by 86.6 %, while the level of humic acids increased by 26.5 %. The elemental analysis showed the reduction of C and the H/C ratio and the increase of O and the O/C ratio of isolated humic acids during the process. The ratio of absorbencies at 465 and 665 nm also increased. Based on this and the Fourier-transform infrared spectra, it was shown that the humic acids isolated at the end of ...bioremediation were enriched with oxygen functional groups and aromatic structures. This study provides one of the first insights into the relationship between bioremediation and humification, as well as evidence of how hydrocarbon-degrading microorganisms have a significant influence on changes to humic acid structure during bioremediation.
Keywords:
remediation / humification / total petroleum hydrocarbonsSource:
Journal of the Serbian Chemical Society, 2020, 85, 6, 821-830Publisher:
- Serbian Chemical Society
Funding / projects:
DOI: 10.2298/JSC190916131Z
ISSN: 0352-5139
WoS: 000548146900010
Scopus: 2-s2.0-85090460119
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Žerađanin, Aleksandra AU - Avdalović, Jelena AU - Lješević, Marija AU - Tešić, Olivera AU - Miletić, Srđan B. AU - Vrvić, Miroslav M. AU - Beškoski, Vladimir PY - 2020 UR - https://cherry.chem.bg.ac.rs/handle/123456789/4234 AB - Environmental pollution is a global problem, while bioremediation technology removes pollutants from the environment using microorganisms. This study was aimed at investigating how a bioremediation process affected soil humification. In soil polluted with petroleum and its derivatives that was submitted to bioremediation, besides the total petroleum hydrocarbons and the number of microorganisms, quantitative and qualitative changes of isolated humic acids were determined during the process. The bioremediation of 150 m3 of polluted soil lasted 150 days. The level of total petroleum hydrocarbons decreased by 86.6 %, while the level of humic acids increased by 26.5 %. The elemental analysis showed the reduction of C and the H/C ratio and the increase of O and the O/C ratio of isolated humic acids during the process. The ratio of absorbencies at 465 and 665 nm also increased. Based on this and the Fourier-transform infrared spectra, it was shown that the humic acids isolated at the end of bioremediation were enriched with oxygen functional groups and aromatic structures. This study provides one of the first insights into the relationship between bioremediation and humification, as well as evidence of how hydrocarbon-degrading microorganisms have a significant influence on changes to humic acid structure during bioremediation. PB - Serbian Chemical Society T2 - Journal of the Serbian Chemical Society T1 - Evolution of humic acids during ex situ bioremediation on a pilot level: The added value of the microbial activity VL - 85 IS - 6 SP - 821 EP - 830 DO - 10.2298/JSC190916131Z ER -
@article{ author = "Žerađanin, Aleksandra and Avdalović, Jelena and Lješević, Marija and Tešić, Olivera and Miletić, Srđan B. and Vrvić, Miroslav M. and Beškoski, Vladimir", year = "2020", abstract = "Environmental pollution is a global problem, while bioremediation technology removes pollutants from the environment using microorganisms. This study was aimed at investigating how a bioremediation process affected soil humification. In soil polluted with petroleum and its derivatives that was submitted to bioremediation, besides the total petroleum hydrocarbons and the number of microorganisms, quantitative and qualitative changes of isolated humic acids were determined during the process. The bioremediation of 150 m3 of polluted soil lasted 150 days. The level of total petroleum hydrocarbons decreased by 86.6 %, while the level of humic acids increased by 26.5 %. The elemental analysis showed the reduction of C and the H/C ratio and the increase of O and the O/C ratio of isolated humic acids during the process. The ratio of absorbencies at 465 and 665 nm also increased. Based on this and the Fourier-transform infrared spectra, it was shown that the humic acids isolated at the end of bioremediation were enriched with oxygen functional groups and aromatic structures. This study provides one of the first insights into the relationship between bioremediation and humification, as well as evidence of how hydrocarbon-degrading microorganisms have a significant influence on changes to humic acid structure during bioremediation.", publisher = "Serbian Chemical Society", journal = "Journal of the Serbian Chemical Society", title = "Evolution of humic acids during ex situ bioremediation on a pilot level: The added value of the microbial activity", volume = "85", number = "6", pages = "821-830", doi = "10.2298/JSC190916131Z" }
Žerađanin, A., Avdalović, J., Lješević, M., Tešić, O., Miletić, S. B., Vrvić, M. M.,& Beškoski, V.. (2020). Evolution of humic acids during ex situ bioremediation on a pilot level: The added value of the microbial activity. in Journal of the Serbian Chemical Society Serbian Chemical Society., 85(6), 821-830. https://doi.org/10.2298/JSC190916131Z
Žerađanin A, Avdalović J, Lješević M, Tešić O, Miletić SB, Vrvić MM, Beškoski V. Evolution of humic acids during ex situ bioremediation on a pilot level: The added value of the microbial activity. in Journal of the Serbian Chemical Society. 2020;85(6):821-830. doi:10.2298/JSC190916131Z .
Žerađanin, Aleksandra, Avdalović, Jelena, Lješević, Marija, Tešić, Olivera, Miletić, Srđan B., Vrvić, Miroslav M., Beškoski, Vladimir, "Evolution of humic acids during ex situ bioremediation on a pilot level: The added value of the microbial activity" in Journal of the Serbian Chemical Society, 85, no. 6 (2020):821-830, https://doi.org/10.2298/JSC190916131Z . .