O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer
Аутори
Jurišević, MilenaJagić, Nikola
Gajović, Nevena
Arsenijević, Aleksandar
Jovanović, Milan
Milovanović, Marija
Pantić, Jelena
Jovanović, Ivan
Sabo, Tibor
Radosavljević, Gordana
Arsenijević, Nebojša
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background/Aim. O,O'-diethyl-(S,S)-ethylenediamineN,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride (DE-EDCP) has been found to possess promising cytotoxic activity against various tumor cell lines. Also, DE-EDCP reduces tumor progression by several mechanisms such as triggering tumor cell death and inhibition of cell proliferation. The aim of present study was to further evaluate antitumor activity of DE-EDCP by investigating effects on migratory potential of tumor cells and anti-tumor immune response. Methods. Migratory potential of DE-EDCP was evaluated by scratch wound assay. Female BALB/c mice were inoculated with 4T1 breast cancer cells and treatment with DE-EDCP started five days following orthotopic tumor implantation. The frequency and phenotype of tumor-infiltrating natural killer (NK) and natural killer T (NKT) cells were analyzed by flow cytometry. Results. DE-EDCP inhibited migratory potential of highly metastatic 4T1 cells. DE-EDCP facilitated accumulation of CD3+CD49+ N...KT cells and CD3-CD49+ NK cells in tumor microenvironment. DE-EDCP treatment led to significant decrement of tumor infiltrating anergic NKT cells expressing cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), killer cell lectin like receptor G1 (KLRG-1) and programmed cell death protein-1 (PD-1). Mice given DE-EDCP had significantly increased percentages of tumoricidal fas ligand (FasL) positive NK cells. Conclusion. DE-EDCP inhibits murine breast cancer progression through direct effects on tumor cells and by facilitating anti-tumor immunity. DE-EDCP enhances accumulation, promotes tumoricidal phenotype and maintenances responsiveness of NK and NKT cells in 4T1 murine breast cancer.
Кључне речи:
breast neoplasms / carcinoma / mice, inbred balbc / antineoplastic agentsИзвор:
Vojnosanitetski pregled, 2020, 77, 7, 715-723Издавач:
- Military Medical Academy, INI
Финансирање / пројекти:
- Молекулске детерминанте урођене имуности у аутоимунским болестима и канцерогенези (RS-MESTD-Basic Research (BR or ON)-175069)
- Развој инфраструктуре за приоритетна поља науке (RS-MESTD-Basic Research (BR or ON)-175103)
- Утицај IL-33/ST2 сигналног пута и галектина-3 у патогенези експерименталних периапикалних промена (RS-MESTD-Basic Research (BR or ON)-175071)
DOI: 10.2298/VSP180723149J
ISSN: 0042-8450
WoS: 000560134500007
Scopus: 2-s2.0-85090430540
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Jurišević, Milena AU - Jagić, Nikola AU - Gajović, Nevena AU - Arsenijević, Aleksandar AU - Jovanović, Milan AU - Milovanović, Marija AU - Pantić, Jelena AU - Jovanović, Ivan AU - Sabo, Tibor AU - Radosavljević, Gordana AU - Arsenijević, Nebojša PY - 2020 UR - https://cherry.chem.bg.ac.rs/handle/123456789/4238 AB - Background/Aim. O,O'-diethyl-(S,S)-ethylenediamineN,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride (DE-EDCP) has been found to possess promising cytotoxic activity against various tumor cell lines. Also, DE-EDCP reduces tumor progression by several mechanisms such as triggering tumor cell death and inhibition of cell proliferation. The aim of present study was to further evaluate antitumor activity of DE-EDCP by investigating effects on migratory potential of tumor cells and anti-tumor immune response. Methods. Migratory potential of DE-EDCP was evaluated by scratch wound assay. Female BALB/c mice were inoculated with 4T1 breast cancer cells and treatment with DE-EDCP started five days following orthotopic tumor implantation. The frequency and phenotype of tumor-infiltrating natural killer (NK) and natural killer T (NKT) cells were analyzed by flow cytometry. Results. DE-EDCP inhibited migratory potential of highly metastatic 4T1 cells. DE-EDCP facilitated accumulation of CD3+CD49+ NKT cells and CD3-CD49+ NK cells in tumor microenvironment. DE-EDCP treatment led to significant decrement of tumor infiltrating anergic NKT cells expressing cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), killer cell lectin like receptor G1 (KLRG-1) and programmed cell death protein-1 (PD-1). Mice given DE-EDCP had significantly increased percentages of tumoricidal fas ligand (FasL) positive NK cells. Conclusion. DE-EDCP inhibits murine breast cancer progression through direct effects on tumor cells and by facilitating anti-tumor immunity. DE-EDCP enhances accumulation, promotes tumoricidal phenotype and maintenances responsiveness of NK and NKT cells in 4T1 murine breast cancer. PB - Military Medical Academy, INI T2 - Vojnosanitetski pregled T1 - O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer VL - 77 IS - 7 SP - 715 EP - 723 DO - 10.2298/VSP180723149J ER -
@article{ author = "Jurišević, Milena and Jagić, Nikola and Gajović, Nevena and Arsenijević, Aleksandar and Jovanović, Milan and Milovanović, Marija and Pantić, Jelena and Jovanović, Ivan and Sabo, Tibor and Radosavljević, Gordana and Arsenijević, Nebojša", year = "2020", abstract = "Background/Aim. O,O'-diethyl-(S,S)-ethylenediamineN,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride (DE-EDCP) has been found to possess promising cytotoxic activity against various tumor cell lines. Also, DE-EDCP reduces tumor progression by several mechanisms such as triggering tumor cell death and inhibition of cell proliferation. The aim of present study was to further evaluate antitumor activity of DE-EDCP by investigating effects on migratory potential of tumor cells and anti-tumor immune response. Methods. Migratory potential of DE-EDCP was evaluated by scratch wound assay. Female BALB/c mice were inoculated with 4T1 breast cancer cells and treatment with DE-EDCP started five days following orthotopic tumor implantation. The frequency and phenotype of tumor-infiltrating natural killer (NK) and natural killer T (NKT) cells were analyzed by flow cytometry. Results. DE-EDCP inhibited migratory potential of highly metastatic 4T1 cells. DE-EDCP facilitated accumulation of CD3+CD49+ NKT cells and CD3-CD49+ NK cells in tumor microenvironment. DE-EDCP treatment led to significant decrement of tumor infiltrating anergic NKT cells expressing cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), killer cell lectin like receptor G1 (KLRG-1) and programmed cell death protein-1 (PD-1). Mice given DE-EDCP had significantly increased percentages of tumoricidal fas ligand (FasL) positive NK cells. Conclusion. DE-EDCP inhibits murine breast cancer progression through direct effects on tumor cells and by facilitating anti-tumor immunity. DE-EDCP enhances accumulation, promotes tumoricidal phenotype and maintenances responsiveness of NK and NKT cells in 4T1 murine breast cancer.", publisher = "Military Medical Academy, INI", journal = "Vojnosanitetski pregled", title = "O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer", volume = "77", number = "7", pages = "715-723", doi = "10.2298/VSP180723149J" }
Jurišević, M., Jagić, N., Gajović, N., Arsenijević, A., Jovanović, M., Milovanović, M., Pantić, J., Jovanović, I., Sabo, T., Radosavljević, G.,& Arsenijević, N.. (2020). O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer. in Vojnosanitetski pregled Military Medical Academy, INI., 77(7), 715-723. https://doi.org/10.2298/VSP180723149J
Jurišević M, Jagić N, Gajović N, Arsenijević A, Jovanović M, Milovanović M, Pantić J, Jovanović I, Sabo T, Radosavljević G, Arsenijević N. O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer. in Vojnosanitetski pregled. 2020;77(7):715-723. doi:10.2298/VSP180723149J .
Jurišević, Milena, Jagić, Nikola, Gajović, Nevena, Arsenijević, Aleksandar, Jovanović, Milan, Milovanović, Marija, Pantić, Jelena, Jovanović, Ivan, Sabo, Tibor, Radosavljević, Gordana, Arsenijević, Nebojša, "O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer" in Vojnosanitetski pregled, 77, no. 7 (2020):715-723, https://doi.org/10.2298/VSP180723149J . .