Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana
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2020
Authors
Vojvodić, SnežanaStanić, Marina

Zechmann, Bernd
Dučić, Tanja
Žižić, Milan
Dimitrijević, Milena
Danilović Luković, Jelena
Milenković, Milica R.

Pittman, Jon K.
Spasojević, Ivan
Article (Published version)

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Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordinat...ion to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.
Keywords:
adaptive response / cell wall / copper / microalgaeSource:
The Biochemical Journal, 2020, 477, 19, 3729-3741Publisher:
- Portland Press
Funding / projects:
- This work is supported by the NATO Science for Peace and Security Programme, Project number G5320.
DOI: 10.1042/BCJ20200600
ISSN: 0264-6021
WoS: 000582387100006
Scopus: 2-s2.0-85092680949
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Vojvodić, Snežana AU - Stanić, Marina AU - Zechmann, Bernd AU - Dučić, Tanja AU - Žižić, Milan AU - Dimitrijević, Milena AU - Danilović Luković, Jelena AU - Milenković, Milica R. AU - Pittman, Jon K. AU - Spasojević, Ivan PY - 2020 UR - https://cherry.chem.bg.ac.rs/handle/123456789/4263 AB - Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper. PB - Portland Press T2 - The Biochemical Journal T1 - Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana VL - 477 IS - 19 SP - 3729 EP - 3741 DO - 10.1042/BCJ20200600 ER -
@article{ author = "Vojvodić, Snežana and Stanić, Marina and Zechmann, Bernd and Dučić, Tanja and Žižić, Milan and Dimitrijević, Milena and Danilović Luković, Jelena and Milenković, Milica R. and Pittman, Jon K. and Spasojević, Ivan", year = "2020", abstract = "Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.", publisher = "Portland Press", journal = "The Biochemical Journal", title = "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana", volume = "477", number = "19", pages = "3729-3741", doi = "10.1042/BCJ20200600" }
Vojvodić, S., Stanić, M., Zechmann, B., Dučić, T., Žižić, M., Dimitrijević, M., Danilović Luković, J., Milenković, M. R., Pittman, J. K.,& Spasojević, I.. (2020). Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in The Biochemical Journal Portland Press., 477(19), 3729-3741. https://doi.org/10.1042/BCJ20200600
Vojvodić S, Stanić M, Zechmann B, Dučić T, Žižić M, Dimitrijević M, Danilović Luković J, Milenković MR, Pittman JK, Spasojević I. Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in The Biochemical Journal. 2020;477(19):3729-3741. doi:10.1042/BCJ20200600 .
Vojvodić, Snežana, Stanić, Marina, Zechmann, Bernd, Dučić, Tanja, Žižić, Milan, Dimitrijević, Milena, Danilović Luković, Jelena, Milenković, Milica R., Pittman, Jon K., Spasojević, Ivan, "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana" in The Biochemical Journal, 477, no. 19 (2020):3729-3741, https://doi.org/10.1042/BCJ20200600 . .