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dc.creatorVojvodić, Snežana
dc.creatorStanić, Marina
dc.creatorZechmann, Bernd
dc.creatorDučić, Tanja
dc.creatorŽižić, Milan
dc.creatorDimitrijević, Milena
dc.creatorDanilović Luković, Jelena
dc.creatorMilenković, Milica R.
dc.creatorPittman, Jon K.
dc.creatorSpasojević, Ivan
dc.date.accessioned2020-12-11T17:55:55Z
dc.date.available2020-12-11T17:55:55Z
dc.date.issued2020
dc.identifier.issn0264-6021
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/4263
dc.description.abstractMicroalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.
dc.languageeng
dc.publisherPortland Press
dc.relationThis work is supported by the NATO Science for Peace and Security Programme, Project number G5320.
dc.rightsrestrictedAccess
dc.sourceThe Biochemical Journal
dc.subjectadaptive response
dc.subjectcell wall
dc.subjectcopper
dc.subjectmicroalgae
dc.titleMechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractСпасојевић, Иван; Станић, Марина; Зецхманн, Бернд; Војводић, Снежана; Дучић, Тања; Даниловић Луковић, Јелена; Питтман, Јон К.; Миленковић, Милица Р.; Димитријевић, Милена; Жижић, Милан;
dc.citation.volume477
dc.citation.issue19
dc.citation.spage3729
dc.citation.epage3741
dc.identifier.wos000582387100006
dc.identifier.doi10.1042/BCJ20200600
dc.citation.rankM21~
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85092680949


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