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dc.creatorMilošević, Jelica
dc.creatorVrhovac, Lidija
dc.creatorĐurković, Filip
dc.creatorJanković, Brankica
dc.creatorMalkov, Saša
dc.creatorLah, Jurij
dc.creatorPolović, Natalija
dc.date.accessioned2020-12-11T18:08:24Z
dc.date.available2020-12-11T18:08:24Z
dc.date.issued2020
dc.identifier.issn1144-0546
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/4264
dc.description.abstractLatex of common fig (Ficus carica) is a rich protein source with a high level of proteolytic activity contributing to its defensive role. The divergent group of cysteine proteases known as ficin (EC 3.4.22.3) represents the majority of latex protein content and shows activity towards fig parasites. Both classical and novel biochemical techniques suggest the intricate pattern of ficin expression and activity profiles. Even though structurally related, different ficin isoforms show some differences in pI values enabling their separation using ion-exchangers. A single alkaline isoform was purified and identified based on the available transcriptomic data as Ficin 1c. This isoform shows both general proteolytic and gelatinolytic activity suggesting a biological role in the degradation of a broad range of natural substrates. The insight into the Ficin 1c structure also provided some functional clues. The secondary structure content and the overall fold are similar to related proteases of the same and other plant sources resulting in similar unfolding routes. Stability assessment of Ficin 1c in comparison to ficin isoform mixture showed that isoform diversity might lead to increased protease stability.
dc.languageen
dc.publisherRoyal Society of Chemistry
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//
dc.rightsrestrictedAccess
dc.sourceNew Journal of Chemistry
dc.titleIsolation, identification, and stability of Ficin 1c isoform from fig latex
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractВрховац, Лидија; Половић, Наталија; Малков, Саша; Јанковић, Бранкица; Лах, Јуриј; Ђурковић, Филип; Милошевић, Јелица;
dc.citation.volume44
dc.citation.issue36
dc.citation.spage15716
dc.citation.epage15723
dc.identifier.wos000571972400046
dc.identifier.doi10.1039/D0NJ02938F
dc.citation.rankM22~
dc.description.otherThe peer-reviewed version: [https://cherry.chem.bg.ac.rs/handle/123456789/4265]
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85092756551


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