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dc.creatorMilošević, Jelica
dc.creatorVrhovac, Lidija
dc.creatorĐurković, Filip
dc.creatorJanković, Brankica
dc.creatorMalkov, Saša
dc.creatorLah, Jurij
dc.creatorPolović, Natalija
dc.date.accessioned2020-12-11T18:14:01Z
dc.date.available2021-09-01
dc.date.issued2020
dc.identifier.issn1144-0546
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/4265
dc.description.abstractLatex of common fig (Ficus carica) is a rich protein source with a high level of proteolytic activity contributing to its defensive role. The divergent group of cysteine proteases known as ficin (EC 3.4.22.3) represents the majority of latex protein content and shows activity towards fig parasites. Both classical and novel biochemical techniques suggest the intricate pattern of ficin expression and activity profiles. Even though structurally related, different ficin isoforms show some differences in pI values enabling their separation using ion-exchangers. A single alkaline isoform was purified and identified based on the available transcriptomic data as Ficin 1c. This isoform shows both general proteolytic and gelatinolytic activity suggesting a biological role in the degradation of a broad range of natural substrates. The insight into the Ficin 1c structure also provided some functional clues. The secondary structure content and the overall fold are similar to related proteases of the same and other plant sources resulting in similar unfolding routes. Stability assessment of Ficin 1c in comparison to ficin isoform mixture showed that isoform diversity might lead to increased protease stability.
dc.languageen
dc.publisherRoyal Society of Chemistry
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//
dc.rightsembargoedAccess
dc.sourceNew Journal of Chemistry
dc.titleIsolation, identification, and stability of Ficin 1c isoform from fig latex
dc.typearticleen
dc.rights.licenseBY-NC-ND
dcterms.abstractМилошевић, Јелица; Малков, Саша; Лах, Јуриј; Ђурковић, Филип; Половић, Наталија; Врховац, Лидија; Јанковић, Бранкица;
dc.citation.volume44
dc.citation.issue36
dc.citation.spage15716
dc.citation.epage15723
dc.identifier.wos000571972400046
dc.identifier.doi10.1039/D0NJ02938F
dc.citation.rankM22~
dc.description.otherThis is the peer-reviewed version of the article: Milošević, J.; Vrhovac, L.; Đurković, F.; Janković, B.; Malkov, S.; Lah, J.; Polović, N. Đ. Isolation, Identification, and Stability of Ficin 1c Isoform from Fig Latex. New J. Chem. 2020, 44 (36), 15716–15723. [https://doi.org/10.1039/D0NJ02938F]
dc.type.versionacceptedVersion
dc.identifier.scopus2-s2.0-85092756551


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