Determination of phenolic profile in kernels of different plum cultivars
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AuthorsFotirić Akšić, Milica
Gašić, Uroš M.
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Large amounts of fruit seeds are discarded yearly in different producing industries, which is a waste of a potentially valuable resource as well as a serious disposal problem. Plum is the most important type of commercial fruit in Serbia and seeds could be obtained as a byproduct of alcoholic beverage processing. Their exploitation should be greater and more information about cultivars' kernels and their composition is required. Also, consumers' tendency for “natural foods” arises a need for characterization of genotypes with high phenolic contents which could be used in processed food products. Discarding large amounts of plum seeds is a waste of potentially precious sources of phytochemicals. In order to characterize the phenolic profile of approximately 30 plum cultivars, phenolic acids and flavonoids, as potential antioxidants, were determined by ultra-high-performance liquid chromatography (UHPLC) coupled with hybrid mass spectrometry, which combines the Linear Trap Quadrupole (LT...Q) and OrbiTrap MS/MS mass analyzer together with chemometric analysis. The UHPLC-LTQ OrbiTrap MS technique was proven to be reliable for the unambiguous detection of phenolic acids, their derivatives, and flavonoid aglycones based on their molecular masses and fragmentation pattern. The phenolic acids prevail over the flavonoids, with protocatechuic acid, p-hydroxybenzoic acid, ferulic acid, and chlorogenic acid as the most abundant ones. In addition, catechin was the most abundant flavonoid.
Keywords:prunus / seed / phenolic acids / flavonoids / UHPLC-LTQ OrbiTrap MS
Source:Acta Horticulturae, 2019, 1260, 229-234
- Leuven : International Society for Horticultural Science
- The Research Council of Norway, as a part of the project No 10662.
- Proc. XI Int. Symposium on Plum and Prune Genetics, Breeding and Pomology