Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors
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2021
Authors
Vrhovac, LidijaŠelemetjev, Sonja A.
Vatić, Saša
Mitrović, Aleksandar
Milošević, Jelica

Lolić, Aleksandar

Beletić, Anđelo D.
Polović, Natalija

Article (Published version)

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Measurement of antithyroglobulin antibodies (TgAb) is an inevitable laboratory tool in the management of thyroid gland diseases. Currently available immunoassays still have limitations underlying the necessity of the introduction of fast, sensitive, and label-free technologies. Our aim was to develop a method for TgAb measurement in human serum based on the quartz crystal microbalance (QCM) technology. We immobilized thyroglobulin on the surface of Attana LNB Carboxyl sensor chip®, prepared standard curve covering the range of 1–50000 kIU/L, and established optimal measurement conditions. The validation included determination of the detection limit (LOD), functional sensitivity, linearity, precision, as well as the comparison with the results of the radioimmunoassay (RIA). The LOD and functional sensitivity were 4.2 kIU/L and 4.7 kIU/L, respectively. The method was linear in the range of 20–10000 kIU/L. The regression equation for comparison with RIA was CQCM = 1.0056 • CRIA – 24.2778,... whereby no significant proportional or systematic difference was present. There was a good agreement with RIA in the classification of patients according to the clinical significance of the results. The developed method has advantages over currently available assays in terms of better LOQ, a higher upper limit of linearity, and precision. The characteristics of the developed method unambiguously show that the application of the QCM biosensors offers a highly reliable novel approach for the measurement of TgAb in human serum.
Keywords:
Antibodies / Biosensor / Human serum / Quartz crystal microbalance / ThyroglobulinSource:
Talanta, 2021, 223, 121588-Publisher:
- Elsevier
Funding / projects:
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-172049)
- Molecular characterization of thyroid gland tumors:biological and clinical aspects (RS-173050)
- Development of new and improvement of existing electrochemical, spectroscopic and flow injection (FIA) methods on environmental quality monitoring (RS-172051)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200168 (University of Belgrade, Faculty of Chemistry) (RS-200168)
DOI: 10.1016/j.talanta.2020.121588
ISSN: 0039-9140
WoS: 000599682400007
Scopus: 2-s2.0-85096881845
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Hemijski fakultetTY - JOUR AU - Vrhovac, Lidija AU - Šelemetjev, Sonja A. AU - Vatić, Saša AU - Mitrović, Aleksandar AU - Milošević, Jelica AU - Lolić, Aleksandar AU - Beletić, Anđelo D. AU - Polović, Natalija PY - 2021 UR - https://cherry.chem.bg.ac.rs/handle/123456789/4295 AB - Measurement of antithyroglobulin antibodies (TgAb) is an inevitable laboratory tool in the management of thyroid gland diseases. Currently available immunoassays still have limitations underlying the necessity of the introduction of fast, sensitive, and label-free technologies. Our aim was to develop a method for TgAb measurement in human serum based on the quartz crystal microbalance (QCM) technology. We immobilized thyroglobulin on the surface of Attana LNB Carboxyl sensor chip®, prepared standard curve covering the range of 1–50000 kIU/L, and established optimal measurement conditions. The validation included determination of the detection limit (LOD), functional sensitivity, linearity, precision, as well as the comparison with the results of the radioimmunoassay (RIA). The LOD and functional sensitivity were 4.2 kIU/L and 4.7 kIU/L, respectively. The method was linear in the range of 20–10000 kIU/L. The regression equation for comparison with RIA was CQCM = 1.0056 • CRIA – 24.2778, whereby no significant proportional or systematic difference was present. There was a good agreement with RIA in the classification of patients according to the clinical significance of the results. The developed method has advantages over currently available assays in terms of better LOQ, a higher upper limit of linearity, and precision. The characteristics of the developed method unambiguously show that the application of the QCM biosensors offers a highly reliable novel approach for the measurement of TgAb in human serum. PB - Elsevier T2 - Talanta T1 - Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors VL - 223 SP - 121588 DO - 10.1016/j.talanta.2020.121588 ER -
@article{ author = "Vrhovac, Lidija and Šelemetjev, Sonja A. and Vatić, Saša and Mitrović, Aleksandar and Milošević, Jelica and Lolić, Aleksandar and Beletić, Anđelo D. and Polović, Natalija", year = "2021", abstract = "Measurement of antithyroglobulin antibodies (TgAb) is an inevitable laboratory tool in the management of thyroid gland diseases. Currently available immunoassays still have limitations underlying the necessity of the introduction of fast, sensitive, and label-free technologies. Our aim was to develop a method for TgAb measurement in human serum based on the quartz crystal microbalance (QCM) technology. We immobilized thyroglobulin on the surface of Attana LNB Carboxyl sensor chip®, prepared standard curve covering the range of 1–50000 kIU/L, and established optimal measurement conditions. The validation included determination of the detection limit (LOD), functional sensitivity, linearity, precision, as well as the comparison with the results of the radioimmunoassay (RIA). The LOD and functional sensitivity were 4.2 kIU/L and 4.7 kIU/L, respectively. The method was linear in the range of 20–10000 kIU/L. The regression equation for comparison with RIA was CQCM = 1.0056 • CRIA – 24.2778, whereby no significant proportional or systematic difference was present. There was a good agreement with RIA in the classification of patients according to the clinical significance of the results. The developed method has advantages over currently available assays in terms of better LOQ, a higher upper limit of linearity, and precision. The characteristics of the developed method unambiguously show that the application of the QCM biosensors offers a highly reliable novel approach for the measurement of TgAb in human serum.", publisher = "Elsevier", journal = "Talanta", title = "Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors", volume = "223", pages = "121588", doi = "10.1016/j.talanta.2020.121588" }
Vrhovac, L., Šelemetjev, S. A., Vatić, S., Mitrović, A., Milošević, J., Lolić, A., Beletić, A. D.,& Polović, N.. (2021). Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors. in Talanta Elsevier., 223, 121588. https://doi.org/10.1016/j.talanta.2020.121588
Vrhovac L, Šelemetjev SA, Vatić S, Mitrović A, Milošević J, Lolić A, Beletić AD, Polović N. Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors. in Talanta. 2021;223:121588. doi:10.1016/j.talanta.2020.121588 .
Vrhovac, Lidija, Šelemetjev, Sonja A., Vatić, Saša, Mitrović, Aleksandar, Milošević, Jelica, Lolić, Aleksandar, Beletić, Anđelo D., Polović, Natalija, "Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors" in Talanta, 223 (2021):121588, https://doi.org/10.1016/j.talanta.2020.121588 . .