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Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants
(Analytical and Bioanalytical Chemistry, 2020)
Resurrection plant Ramonda serbica is a suitable model to investigate vegetative desiccation tolerance. However, the detailed study of these mechanisms at the protein level is hampered by the severe tissue water loss, high ...
In silico structural survey of newly identified late embryogenesis abundant proteins (LEAPs) from Ramonda serbica and their structure - function relationship
(Virtual symposium celebrating the 50th anniversary of the Protein Data Bank, May 4–5, 2021)
Desiccation or extreme water loss leads to protein denaturation, aggregation, and degradation and impairs membrane lipid fluidity, resulting in loss of membrane integrity at the cellular level. The induction of late ...
De Novo Transcriptome Sequencing of Ramonda serbica : Identification of Late Embryogenesis Abundant Proteins
(Belgrade BioInformatics, 2021)
In silico structural survey of newly identified late embryogenesis abundant proteins (LEAPs) from Ramonda serbica and their structure - function relationship
(Virtual symposium celebrating the 50th anniversary of the Protein Data Bank, May 4–5, 2021)
Desiccation or extreme water loss leads to protein denaturation, aggregation, and degradation and impairs membrane lipid fluidity, resulting in loss of membrane integrity at the cellular level. The induction of late ...
Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects
(4th International Conference on Plant Biology, Book of Abstracts, 2022)
Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a long
period and fully resume metabolic functions upon rewatering in a very short period, even within
48 h. The goal of this study ...
Ekspresija, prečišćavanje i karakterizacija izoforme H8 lignin peroksidaze u kvascu Pichia pastoris
(2021)
Cilj ovog rada je kloniranje i ekspresija izoforme H8 lignin peroksidaze u kvascu Pichia
pastoris soj KM71H. Dobijeni enzim je prečišćen upotrebom DEAE kolone, a potom detaljno
kinetički okarakterisan i definisani su mu ...
Optimizacija protokola proizvodnje proteina zastupljenog u kasnoj fazi embriogeneze (RsLEA_30) iz biljke vaskrsnice Ramonda serbica tehnikom rekombinantne DNK u bakteriji Escherichia coli
(2021)
Cilj ove master teze je optimizacija procesa dobijanja prečišćenog proteina zastupljenog u kasnoj fazi embriogeneze (LEAP) iz biljke vaskrsnice Ramonda serbica tehnologijom rekombinantne DNK. Ova procedura obezbeđuje ...
Optimizacija protokola za efikasnu proizvodnju proteina zastupljenog u kasnoj fazi embriogeneze (AtLEA_25) iz biljke Arabidopsis thaliana tehnologijom rekombinantne DNK
(2021)
Cilj ovog master rada je optimizacija procesa dobijanja prečišćenog LEA proteina (AtLEA25) iz biljke Arabidopsis thaliana tehnologijom rekombinantne DNA. Ovom procedurom je potrebno dobiti značajne količine LEA proteina ...
Razvoj Antigenskog Testa za Detekciju Nukleokapsidnog Proteina SARS-CoV-2
(2022)
SARS-CoV-2 je virus koji je uzročnik pandemije kovid-19. Virus ima visoku stopu smrtnosti, pogotovo kod osetljivih grupa, i veoma je zarazan. Lako se širi te je detekcija virusa , a potom i izolacija obolelih ključan korak ...