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Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2
dc.creator | Đukić, Teodora | |
dc.creator | Mladenović, Maja | |
dc.creator | Stanić-Vučinić, Dragana | |
dc.creator | Radosavljević, Jelena | |
dc.creator | Smiljanić, Katarina | |
dc.creator | Sabljić, Ljiljana | |
dc.creator | Dević, Marija | |
dc.creator | Ćujić, Danica R. | |
dc.creator | Vasović, Tamara | |
dc.creator | Simović, Ana | |
dc.creator | Radomirović, Mirjana Ž. | |
dc.creator | Ćirković-Veličković, Tanja | |
dc.date.accessioned | 2021-02-28T16:33:06Z | |
dc.date.available | 2021-02-28T16:33:06Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0042-6822 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/4330 | |
dc.description.abstract | Serological testing is important method for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production. SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58–419 aa) was expressed in E. coli in soluble form, purified and characterized biochemically and immunologically. Purified rfNP has secondary structure of full-length recombinant N protein, with high percentage of disordered structure (34.2%) and of β-sheet (40.7%). rfNP was tested in immunoblot using sera of COVID-19 convalescent patients. ELISA was optimized with sera of RT-PCR confirmed positive symptomatic patients and healthy individuals. IgG detection sensitivity was 96% (47/50) and specificity 97% (67/68), while IgM detection was slightly lower (94% and 96.5%, respectively). Cost-effective approach for soluble recombinant N protein fragment production was developed, with reliable IgG and IgM antibodies detection of SARS-CoV-2 infection. | sr |
dc.language.iso | en | sr |
dc.publisher | Elsevier | sr |
dc.relation | info:eu-repo/grantAgreement/ScienceFundRS/Fond_2020_COVID19/7542203/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS// | sr |
dc.relation | Serbian Academy of Sciences and Arts GA No. F-26. | sr |
dc.relation | UNDP project "Preventing and Responding to COVID-19 in At-risk areas - Sustainable production of the serological IgG test for SARS CoV-2 in Serbia". | sr |
dc.relation.isversionof | https://cherry.chem.bg.ac.rs/handle/123456789/4331 | |
dc.rights | embargoedAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Virology journal | sr |
dc.subject | Recombinant nucleocapsid protein | sr |
dc.subject | COVID-19 | sr |
dc.subject | SARS-CoV-2 | sr |
dc.subject | Prokaryotic expression | sr |
dc.subject | serological assay | sr |
dc.title | Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2 | sr |
dc.type | article | sr |
dc.rights.license | BY-NC-ND | sr |
dcterms.abstract | Ћујић, Даница; Ђукић, Теодора; Младеновић, Маја; Станић-Вучинић, Драгана; Радосављевић, Јелена; Смиљанић, Катарина; Сабљић, Љиљана; Девић, Марија; Васовић, Тамара; Симовић, Aна; Радомировић, Мирјана Ж.; Ћирковић-Величковић, Тања; | |
dc.citation.volume | 557 | |
dc.citation.spage | 15 | |
dc.citation.epage | 22 | |
dc.identifier.wos | 000632032400001 | |
dc.identifier.doi | 10.1016/j.virol.2021.01.004 | |
dc.citation.rank | M22~ | |
dc.description.other | This is the preprint of the article: Đukić, T.; Mladenović, M.; Stanić-Vučinić, D.; Radosavljević, J.; Smiljanić, K.; Sabljić, L.; Dević, M.; Ćujić, D.; Vasović, T.; Simović, A.; Radomirović, M. Ž.; Ćirković-Veličković, T. Expression, Purification and Immunological Characterization of Recombinant Nucleocapsid Protein Fragment from SARS-CoV-2. Virology journal 2021, 557, 15–22. [https://doi.org/10.1016/j.virol.2021.01.004]. | |
dc.type.version | submittedVersion | sr |
dc.identifier.scopus | 2-s2.0-85100810713 |