Hepatoprotective Effect of Mixture of Dipropyl Polysulfides in Concanavalin A-Induced Hepatitis
Authors
Arsenijević, Dragana
Stojanović, Bojana
Milovanović, Jelena

Arsenijević, Aleksandar
Simić, Miloš
Pergal, Marija V.

Kodranov, Igor D.

Cvetković, Olga
Vojvodić, Danilo
Ristanović, Elizabeta
Manojlović, Dragan D.

Milovanović, Marija
Arsenijević, Nebojša

Article (Published version)
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The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysulfides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while ...the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, characterized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases.
Keywords:
anti-inflammatory activity / ConA hepatitis / dipropyl polysulfides / hepatoprotective effectsSource:
Nutrients, 2021, 13, 3, 1022-Publisher:
- MDPI
Funding / projects:
- Molecular determinants of innate immunity in autoimmunity and tumorogenesis (RS-175069)
- Oxide-based environmentally-friendly porous materials for genotoxic substances removal (RS-172018)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) (RS-200026)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200168 (University of Belgrade, Faculty of Chemistry) (RS-200168)
- Serbian bilateral project with PR China (06/2018).
- The Faculty of Medical Sciences, University of Kragujevac (MP 01/19 and JP 22/19).
- Grants No. ON175069
- Grants No. ON172018
DOI: 10.3390/nu13031022
ISSN: 2072-6643
WoS: 000633943600001
Scopus: 2-s2.0-85102752250
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Arsenijević, Dragana AU - Stojanović, Bojana AU - Milovanović, Jelena AU - Arsenijević, Aleksandar AU - Simić, Miloš AU - Pergal, Marija V. AU - Kodranov, Igor D. AU - Cvetković, Olga AU - Vojvodić, Danilo AU - Ristanović, Elizabeta AU - Manojlović, Dragan D. AU - Milovanović, Marija AU - Arsenijević, Nebojša PY - 2021 UR - UR - https://cherry.chem.bg.ac.rs/handle/123456789/4375 AB - The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysulfides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, characterized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases. PB - MDPI T2 - Nutrients T2 - Nutrients T1 - Hepatoprotective Effect of Mixture of Dipropyl Polysulfides in Concanavalin A-Induced Hepatitis VL - 13 IS - 3 SP - 1022 EP - DO - 10.3390/nu13031022 ER -
@article{ author = "Arsenijević, Dragana and Stojanović, Bojana and Milovanović, Jelena and Arsenijević, Aleksandar and Simić, Miloš and Pergal, Marija V. and Kodranov, Igor D. and Cvetković, Olga and Vojvodić, Danilo and Ristanović, Elizabeta and Manojlović, Dragan D. and Milovanović, Marija and Arsenijević, Nebojša", year = "2021", abstract = "The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysulfides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, characterized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases.", publisher = "MDPI", journal = "Nutrients, Nutrients", title = "Hepatoprotective Effect of Mixture of Dipropyl Polysulfides in Concanavalin A-Induced Hepatitis", volume = "13", number = "3", pages = "1022-", doi = "10.3390/nu13031022" }
Arsenijević, D., Stojanović, B., Milovanović, J., Arsenijević, A., Simić, M., Pergal, M. V., Kodranov, I. D., Cvetković, O., Vojvodić, D., Ristanović, E., Manojlović, D. D., Milovanović, M.,& Arsenijević, N.. (2021). Hepatoprotective Effect of Mixture of Dipropyl Polysulfides in Concanavalin A-Induced Hepatitis. in Nutrients MDPI., 13(3), 1022-. https://doi.org/10.3390/nu13031022
Arsenijević D, Stojanović B, Milovanović J, Arsenijević A, Simić M, Pergal MV, Kodranov ID, Cvetković O, Vojvodić D, Ristanović E, Manojlović DD, Milovanović M, Arsenijević N. Hepatoprotective Effect of Mixture of Dipropyl Polysulfides in Concanavalin A-Induced Hepatitis. in Nutrients. 2021;13(3):1022-. doi:10.3390/nu13031022 .
Arsenijević, Dragana, Stojanović, Bojana, Milovanović, Jelena, Arsenijević, Aleksandar, Simić, Miloš, Pergal, Marija V., Kodranov, Igor D., Cvetković, Olga, Vojvodić, Danilo, Ristanović, Elizabeta, Manojlović, Dragan D., Milovanović, Marija, Arsenijević, Nebojša, "Hepatoprotective Effect of Mixture of Dipropyl Polysulfides in Concanavalin A-Induced Hepatitis" in Nutrients, 13, no. 3 (2021):1022-, https://doi.org/10.3390/nu13031022 . .