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dc.creatorStevanović, Nikola R.
dc.creatorApostolović, Danijela
dc.creatorMilčić, Miloš K.
dc.creatorLolić, Aleksandar
dc.creatorHage, Marianne van
dc.creatorĆirković-Veličković, Tanja
dc.creatorBaošić, Rada
dc.date.accessioned2021-04-27T09:31:15Z
dc.date.available2021-04-27T09:31:15Z
dc.date.issued2021
dc.identifier.issn1369-9261
dc.identifier.urihttps://pubs.rsc.org/en/content/articlelanding/2021/nj/d1nj00040c
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/4399
dc.description.abstractDifferent Schiff base complexes have biological activities that make them suitable for drug design. The biological properties of tetradentate Schiff base copper(II) complexed with N,N′-bis(acetylacetone)propylenediimine have been studied. The cytotoxic activity towards Caco-2 cells were determined by MTT, Anexin V and PI apoptosis assays. N,N′-bis(acetylacetone)propylenediimine-copper(II) showed the anti-cancer and anti-proliferative properties by inducing apoptosis in Caco-2 cells. A comparison of the cytotoxic activity of this compound with cisplatin shows that it is more effective on the colorectal cancer cell line Caco-2. The binding capacity and interaction of N,N′-bis(acetylacetone)propylenediimine-copper(II) with HSA were systemically investigated by in vitro fluorescence spectroscopy, CD spectroscopy, and in silico molecular docking study. Furthermore, in vitro and in silico interaction studies indicated that the complex binds to HSA through a static quenching mechanism without changes in protein conformation. The calculated number of binding sites was in line with molecular docking studies. The obtained Ka value suggests that the compound can be released from the protein in target cells. The tetradentate Schiff base copper(II) complex exhibited in vitro biological activities against cancer epithelial cells, which depend on the molecular structure of the complex, causing apoptosis, and the complex can bind to the protein drug carrier in circulation to the target tissue.
dc.languageen
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/810752/EU//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//
dc.relation.isreferencedbyhttps://cherry.chem.bg.ac.rs/handle/123456789/4400
dc.relation.isreplacedbyhttps://cherry.chem.bg.ac.rs/handle/123456789/4400
dc.rightsrestrictedAccess
dc.sourceNew Journal of Chemistry
dc.titleInteraction, binding capacity and anticancer properties of N,N′-bis(acetylacetone)-propylenediimine-copper(II) on colorectal cancer cell line Caco-2
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractСтевановић, Никола; Баошић, Рада; Aпостоловић, Данијела; Лолић, Aлександар; Ћирковић-Величковић, Тања; Милчић, Милош; Хаге, Марианне ван;
dcterms.publisherRoyal Society of Chemistry
dc.citation.volume45
dc.citation.issue14
dc.citation.spage6231
dc.citation.epage6237
dc.identifier.wos000631723200001
dc.identifier.doi10.1039/D1NJ00040C
dc.description.otherSupplementary material: [https://cherry.chem.bg.ac.rs/handle/123456789/4400]
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-85104002973


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