Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen
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2021
Authors
Kostić, Aleksandar Ž.
Milinčić, Danijel D.

Stanisavljević, Nemanja S.
Gašić, Uroš M.

Lević, Steva M.
Kojić, Milan O.

Tešić, Živoslav Lj.

Nedović, Viktor A.

Barać, Miroljub B.

Pešić, Mirjana B.

Article (Published version)

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The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7–76.2%) followed by phenolic acids (14.2–17.4%). Among individual compounds quercetin-3-O-glucoside (155.1–197.2 μg/L) and p-coumaric acid (29.5–30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelatin...g complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient. © 2021 Elsevier Ltd
Keywords:
Antioxidant properties / Goat milk/pollen powder / In vitro polyphenol bioaccessibility / Monofloral sunflower bee-collected pollenSource:
Food Chemistry, 2021, 351, 129310-Publisher:
- Elsevier
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200116 (University of Belgrade, Faculty of Agriculture) (RS-200116)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-200007)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-200042)
Note:
- Supplementary material: https://cherry.chem.bg.ac.rs/handle/123456789/4408
Related info:
- Version of
https://doi.org/10.1016/j.foodchem.2021.129310 - Referenced by
https://cherry.chem.bg.ac.rs/handle/123456789/4408
DOI: 10.1016/j.foodchem.2021.129310
ISSN: 0308-8146
WoS: 000632300600001
Scopus: 2-s2.0-85101327994
URI
https://www.sciencedirect.com/science/article/pii/S0308814621003162?via%3Dihubhttps://cherry.chem.bg.ac.rs/handle/123456789/4407
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Kostić, Aleksandar Ž. AU - Milinčić, Danijel D. AU - Stanisavljević, Nemanja S. AU - Gašić, Uroš M. AU - Lević, Steva M. AU - Kojić, Milan O. AU - Tešić, Živoslav Lj. AU - Nedović, Viktor A. AU - Barać, Miroljub B. AU - Pešić, Mirjana B. PY - 2021 UR - https://www.sciencedirect.com/science/article/pii/S0308814621003162?via%3Dihub UR - https://cherry.chem.bg.ac.rs/handle/123456789/4407 AB - The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7–76.2%) followed by phenolic acids (14.2–17.4%). Among individual compounds quercetin-3-O-glucoside (155.1–197.2 μg/L) and p-coumaric acid (29.5–30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient. © 2021 Elsevier Ltd PB - Elsevier T2 - Food Chemistry T2 - Food Chemistry T1 - Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen VL - 351 SP - 129310 DO - 10.1016/j.foodchem.2021.129310 ER -
@article{ author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Stanisavljević, Nemanja S. and Gašić, Uroš M. and Lević, Steva M. and Kojić, Milan O. and Tešić, Živoslav Lj. and Nedović, Viktor A. and Barać, Miroljub B. and Pešić, Mirjana B.", year = "2021", abstract = "The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7–76.2%) followed by phenolic acids (14.2–17.4%). Among individual compounds quercetin-3-O-glucoside (155.1–197.2 μg/L) and p-coumaric acid (29.5–30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient. © 2021 Elsevier Ltd", publisher = "Elsevier", journal = "Food Chemistry, Food Chemistry", title = "Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen", volume = "351", pages = "129310", doi = "10.1016/j.foodchem.2021.129310" }
Kostić, A. Ž., Milinčić, D. D., Stanisavljević, N. S., Gašić, U. M., Lević, S. M., Kojić, M. O., Tešić, Ž. Lj., Nedović, V. A., Barać, M. B.,& Pešić, M. B.. (2021). Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen. in Food Chemistry Elsevier., 351, 129310. https://doi.org/10.1016/j.foodchem.2021.129310
Kostić AŽ, Milinčić DD, Stanisavljević NS, Gašić UM, Lević SM, Kojić MO, Tešić ŽL, Nedović VA, Barać MB, Pešić MB. Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen. in Food Chemistry. 2021;351:129310. doi:10.1016/j.foodchem.2021.129310 .
Kostić, Aleksandar Ž., Milinčić, Danijel D., Stanisavljević, Nemanja S., Gašić, Uroš M., Lević, Steva M., Kojić, Milan O., Tešić, Živoslav Lj., Nedović, Viktor A., Barać, Miroljub B., Pešić, Mirjana B., "Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen" in Food Chemistry, 351 (2021):129310, https://doi.org/10.1016/j.foodchem.2021.129310 . .