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dc.creatorMandić, Ljuba M.
dc.creatorStojanović, Srđan Đ.
dc.creatorColovic, A
dc.creatorMilin, N
dc.date.accessioned2018-11-22T00:03:23Z
dc.date.available2018-11-22T00:03:23Z
dc.date.issued2000
dc.identifier.issn0354-3447
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/445
dc.description.abstractThe estimation of glycosylated proteins is useful in the detection and control of long-standing hyperglycemic conditions. In this paper the content of protein-bound glucose was determined in diabetics by spectrophotometric method with phenol-sulphuric acid (PS) and with thiobarbituric acid (TBA). The amounts of protein-bound glucose obtained with the PS reagent (80.6 +/- 22.8 mmol glucose/g protein) were always considerably higher than those obtained by TEA method (3.71 +/- 1.34 mu mol glucose/g protein). The analysis was made of parameters that might have led to such significant difference. It was shown that in the reaction with PS reagent there was an additional dehydration of carbohydrate released during the hydrolysis of glycosylated proteins. The additional dehydration was due to concentrated sulphuric acid which at higher temperature influenced chromogen formation. On the basis of the results obtained it was concluded that, although the sensitivity of the both methods for standard fructose was comparable, TEA method was more reliable than PS method. Therefore, it is recommended in clinical practice.en
dc.publisherSoc Medical Biochemists Yugoslavia, Belgrade
dc.rightsrestrictedAccess
dc.sourceJugoslovenska Medicinska Biohemija
dc.subjectglycosylated proteinsen
dc.subjectphenol-sulphuric methoden
dc.subjectthiobarbituric methoden
dc.titleDetermination of serum glycosylated proteins: Comparison of thiobarbituric acid and phenol-sulphuric acid assaysen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМандић, Љуба М.; Милин, Н; Цоловиц, A; Стојановиц, С;
dc.citation.volume19
dc.citation.issue4
dc.citation.spage411
dc.citation.epage416
dc.identifier.wos000166041900007
dc.citation.other19(4): 411-416
dc.citation.rankM23
dc.type.versionpublishedVersionen
dc.identifier.rcubKon_1419


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