Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application
Аутори
Đurđić, Slađana Z.Stanković, V.
Vlahović, Filip
Ognjanović, Miloš
Kalcher, K.
Ćirković-Veličković, Tanja
Mutić, Jelena
Stanković, Dalibor
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
Based on graphene nanoplatelets capability to build block composites, as well as well-known electrochemical characteristic of the manganese oxide materials, in the present research, a nanocomposite, formed from graphene nanoplatelets (GNP) and manganese(IV)-oxide (MnO2) nanoparticles, has been proposed as a novel and convenient support for enzyme immobilization. Performance of screen printed carbon electrodes (SPCEs) was significantly improved after their modification with GNP@MnO2 (SPCE/GNP@MnO2). The polyphenolic index biosensor was prepared by applying the drop coating technique using laccase and Nafion®. Developed biosensor shows a fast and reliable amperometric response toward caffeic acid, as a model compound, at operating potential of +0.40 V (vs Ag/AgCl), with a wide linear range and detection limit of 1.9 μmol l−1. Developed procedure was successfully applied for the determination of polyphenolic indexes in wine samples. Recovery tests indicate excellent accuracy and precision... of the method, concluding that the biosensor can offer a fast, accurate, reliable and precise determination of the polyphenolic index. More importantly, our results suggest a great potential for the application in real samples.
Извор:
Journal of The Electrochemical Society, 2021, 168, 3, 037510-Издавач:
- The Electrochemical Society (ECS)
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-200168)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-200026)
- CEEPUS network CIII-CZ-0212-13-1920-M- 131892 (Education of Modern Analytical and Bioanalytical Methods).
- Eureka project call, E! 13303 MED-BIO-TEST.
Напомена:
- This is the peer-reviewed version of the article: Đurđić, S., Stanković, V., Vlahović, F., Ognjanović, M., Kalcher, K., Ćirković Veličković, T., Mutić, J. J.,& Stanković, D. M. (2021). Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application. Journal of Electrochemical Society, 168(3), 037510. https://doi.org/10.1149/1945-7111/abeaf2
- Published version: https://cherry.chem.bg.ac.rs/handle/123456789/4474
Повезане информације:
- Верзија документа
https://doi.org/10.1149/1945-7111/abeaf2 - Верзија документа
https://cherry.chem.bg.ac.rs/handle/123456789/4474
DOI: 10.1149/1945-7111/abeaf2
ISSN: 0013-4651
WoS: 000629756800001
Scopus: 2-s2.0-85103235625
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Đurđić, Slađana Z. AU - Stanković, V. AU - Vlahović, Filip AU - Ognjanović, Miloš AU - Kalcher, K. AU - Ćirković-Veličković, Tanja AU - Mutić, Jelena AU - Stanković, Dalibor PY - 2021 UR - https://cherry.chem.bg.ac.rs/handle/123456789/4475 AB - Based on graphene nanoplatelets capability to build block composites, as well as well-known electrochemical characteristic of the manganese oxide materials, in the present research, a nanocomposite, formed from graphene nanoplatelets (GNP) and manganese(IV)-oxide (MnO2) nanoparticles, has been proposed as a novel and convenient support for enzyme immobilization. Performance of screen printed carbon electrodes (SPCEs) was significantly improved after their modification with GNP@MnO2 (SPCE/GNP@MnO2). The polyphenolic index biosensor was prepared by applying the drop coating technique using laccase and Nafion®. Developed biosensor shows a fast and reliable amperometric response toward caffeic acid, as a model compound, at operating potential of +0.40 V (vs Ag/AgCl), with a wide linear range and detection limit of 1.9 μmol l−1. Developed procedure was successfully applied for the determination of polyphenolic indexes in wine samples. Recovery tests indicate excellent accuracy and precision of the method, concluding that the biosensor can offer a fast, accurate, reliable and precise determination of the polyphenolic index. More importantly, our results suggest a great potential for the application in real samples. PB - The Electrochemical Society (ECS) T2 - Journal of The Electrochemical Society T1 - Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application VL - 168 IS - 3 SP - 037510 DO - 10.1149/1945-7111/abeaf2 ER -
@article{ author = "Đurđić, Slađana Z. and Stanković, V. and Vlahović, Filip and Ognjanović, Miloš and Kalcher, K. and Ćirković-Veličković, Tanja and Mutić, Jelena and Stanković, Dalibor", year = "2021", abstract = "Based on graphene nanoplatelets capability to build block composites, as well as well-known electrochemical characteristic of the manganese oxide materials, in the present research, a nanocomposite, formed from graphene nanoplatelets (GNP) and manganese(IV)-oxide (MnO2) nanoparticles, has been proposed as a novel and convenient support for enzyme immobilization. Performance of screen printed carbon electrodes (SPCEs) was significantly improved after their modification with GNP@MnO2 (SPCE/GNP@MnO2). The polyphenolic index biosensor was prepared by applying the drop coating technique using laccase and Nafion®. Developed biosensor shows a fast and reliable amperometric response toward caffeic acid, as a model compound, at operating potential of +0.40 V (vs Ag/AgCl), with a wide linear range and detection limit of 1.9 μmol l−1. Developed procedure was successfully applied for the determination of polyphenolic indexes in wine samples. Recovery tests indicate excellent accuracy and precision of the method, concluding that the biosensor can offer a fast, accurate, reliable and precise determination of the polyphenolic index. More importantly, our results suggest a great potential for the application in real samples.", publisher = "The Electrochemical Society (ECS)", journal = "Journal of The Electrochemical Society", title = "Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application", volume = "168", number = "3", pages = "037510", doi = "10.1149/1945-7111/abeaf2" }
Đurđić, S. Z., Stanković, V., Vlahović, F., Ognjanović, M., Kalcher, K., Ćirković-Veličković, T., Mutić, J.,& Stanković, D.. (2021). Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application. in Journal of The Electrochemical Society The Electrochemical Society (ECS)., 168(3), 037510. https://doi.org/10.1149/1945-7111/abeaf2
Đurđić SZ, Stanković V, Vlahović F, Ognjanović M, Kalcher K, Ćirković-Veličković T, Mutić J, Stanković D. Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application. in Journal of The Electrochemical Society. 2021;168(3):037510. doi:10.1149/1945-7111/abeaf2 .
Đurđić, Slađana Z., Stanković, V., Vlahović, Filip, Ognjanović, Miloš, Kalcher, K., Ćirković-Veličković, Tanja, Mutić, Jelena, Stanković, Dalibor, "Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application" in Journal of The Electrochemical Society, 168, no. 3 (2021):037510, https://doi.org/10.1149/1945-7111/abeaf2 . .