Dopamine-modified pectin for a Streptomyces cyaneus laccase induced microbeads formation, immobilization, and textile dyes decolorization
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2021
Authors
Popović, Nikolina
Stanišić, Marija D.

Ilić Đurđić, Karla

Prodanović, Olivera
Polović, Natalija

Prodanović, Radivoje

Article (Published version)

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Pectins are a group of heterologous polysaccharides capable of forming hydrogels and applicable in many industrial processes. A new type of modified pectin was synthesized by periodate oxidation and reductive amination with dopamine and sodium cyanoborohydride. The success of modification was confirmed by UV–Vis,FTIR, and 1H NMR spectroscopy. The obtained dopamine-pectin could form hydrogels by ionic crosslinking of carboxyl groups with calcium or by crosslinking phenol groups with laccase. For enzymatic crosslinking with laccase from Streptomyces cyaneus expressed in E. coli, isolation and purification of the enzyme was done. Using emulsion-based enzymatic crosslinking polymerization, dopamine-pectin microbeads with immobilized laccase were made. The immobilized laccase showed improved thermal and pH stability in comparison to the free enzyme. The immobilized biocatalyst effectively decolorized various dyes: Amido Black 10B, Reactive Black 5, and Evans Blue. After ten cycles of repeat...ed use, the microbead immobilized laccase could still decolorize 60% and 36% of Amido Black 10B and Reactive Black 5, respectively.
Keywords:
Bioremediation / Dyes decolorization / Immobilization / Laccase / Modified pectinsSource:
Environmental Technology & Innovation, 2021, 22, 101399-Publisher:
- Elsevier
Funding / projects:
- Razvoj i primena proizvoda na bazi mineralnih sirovina u proizvodnji bezbedne hrane (RS-20016)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
Note:
- Supplementary material: https://cherry.chem.bg.ac.rs/handle/123456789/4494
Related info:
- Referenced by
https://cherry.chem.bg.ac.rs/handle/123456789/4494
DOI: 10.1016/j.eti.2021.101399
ISSN: 2352-1864
WoS: 000647725600019
Scopus: 2-s2.0-85100426099
URI
https://www.sciencedirect.com/science/article/pii/S235218642100047Xhttps://cherry.chem.bg.ac.rs/handle/123456789/4493
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Popović, Nikolina AU - Stanišić, Marija D. AU - Ilić Đurđić, Karla AU - Prodanović, Olivera AU - Polović, Natalija AU - Prodanović, Radivoje PY - 2021 UR - https://www.sciencedirect.com/science/article/pii/S235218642100047X UR - https://cherry.chem.bg.ac.rs/handle/123456789/4493 AB - Pectins are a group of heterologous polysaccharides capable of forming hydrogels and applicable in many industrial processes. A new type of modified pectin was synthesized by periodate oxidation and reductive amination with dopamine and sodium cyanoborohydride. The success of modification was confirmed by UV–Vis,FTIR, and 1H NMR spectroscopy. The obtained dopamine-pectin could form hydrogels by ionic crosslinking of carboxyl groups with calcium or by crosslinking phenol groups with laccase. For enzymatic crosslinking with laccase from Streptomyces cyaneus expressed in E. coli, isolation and purification of the enzyme was done. Using emulsion-based enzymatic crosslinking polymerization, dopamine-pectin microbeads with immobilized laccase were made. The immobilized laccase showed improved thermal and pH stability in comparison to the free enzyme. The immobilized biocatalyst effectively decolorized various dyes: Amido Black 10B, Reactive Black 5, and Evans Blue. After ten cycles of repeated use, the microbead immobilized laccase could still decolorize 60% and 36% of Amido Black 10B and Reactive Black 5, respectively. PB - Elsevier T2 - Environmental Technology & Innovation T2 - Environmental Technology & InnovationEnvironmental Technology & Innovation T1 - Dopamine-modified pectin for a Streptomyces cyaneus laccase induced microbeads formation, immobilization, and textile dyes decolorization VL - 22 SP - 101399 DO - 10.1016/j.eti.2021.101399 ER -
@article{ author = "Popović, Nikolina and Stanišić, Marija D. and Ilić Đurđić, Karla and Prodanović, Olivera and Polović, Natalija and Prodanović, Radivoje", year = "2021", abstract = "Pectins are a group of heterologous polysaccharides capable of forming hydrogels and applicable in many industrial processes. A new type of modified pectin was synthesized by periodate oxidation and reductive amination with dopamine and sodium cyanoborohydride. The success of modification was confirmed by UV–Vis,FTIR, and 1H NMR spectroscopy. The obtained dopamine-pectin could form hydrogels by ionic crosslinking of carboxyl groups with calcium or by crosslinking phenol groups with laccase. For enzymatic crosslinking with laccase from Streptomyces cyaneus expressed in E. coli, isolation and purification of the enzyme was done. Using emulsion-based enzymatic crosslinking polymerization, dopamine-pectin microbeads with immobilized laccase were made. The immobilized laccase showed improved thermal and pH stability in comparison to the free enzyme. The immobilized biocatalyst effectively decolorized various dyes: Amido Black 10B, Reactive Black 5, and Evans Blue. After ten cycles of repeated use, the microbead immobilized laccase could still decolorize 60% and 36% of Amido Black 10B and Reactive Black 5, respectively.", publisher = "Elsevier", journal = "Environmental Technology & Innovation, Environmental Technology & InnovationEnvironmental Technology & Innovation", title = "Dopamine-modified pectin for a Streptomyces cyaneus laccase induced microbeads formation, immobilization, and textile dyes decolorization", volume = "22", pages = "101399", doi = "10.1016/j.eti.2021.101399" }
Popović, N., Stanišić, M. D., Ilić Đurđić, K., Prodanović, O., Polović, N.,& Prodanović, R.. (2021). Dopamine-modified pectin for a Streptomyces cyaneus laccase induced microbeads formation, immobilization, and textile dyes decolorization. in Environmental Technology & Innovation Elsevier., 22, 101399. https://doi.org/10.1016/j.eti.2021.101399
Popović N, Stanišić MD, Ilić Đurđić K, Prodanović O, Polović N, Prodanović R. Dopamine-modified pectin for a Streptomyces cyaneus laccase induced microbeads formation, immobilization, and textile dyes decolorization. in Environmental Technology & Innovation. 2021;22:101399. doi:10.1016/j.eti.2021.101399 .
Popović, Nikolina, Stanišić, Marija D., Ilić Đurđić, Karla, Prodanović, Olivera, Polović, Natalija, Prodanović, Radivoje, "Dopamine-modified pectin for a Streptomyces cyaneus laccase induced microbeads formation, immobilization, and textile dyes decolorization" in Environmental Technology & Innovation, 22 (2021):101399, https://doi.org/10.1016/j.eti.2021.101399 . .