S-Adenosyl-L-Homocysteine (AdoHcy) is a crucial pathological factor in homocysteine-associated disorders. We developed an impedimetric approach for single drop S-Adenosyl-L-Homocysteine detection, based on S-Adenosyl-L-Homocysteine hydrolase (SAH), produced in the prokaryotic expression system (E. coli) by recombinant DNA technology, immobilized on citrate acid-coated gallium oxyhydroxide dispersed over single-walled carbon nanotubes (CA/GaO(OH)@SWCNT). The proposed biosensor offers detection of AdoHcy, with a limit of detection (LOD) of 0.17 μM. with operating linear range from 1 to 44 μM. The remarkable stability of gallium oxyhydroxide crystal was further improved by citric acid coating and its optical, electrical and physical anisotropy was skipped using SWCNTs.
This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia through Eureka project E! 13303 MED-BIO-TEST.
TY - JOUR
AU - Knežević, Sara
AU - Ognjanović, Miloš
AU - Gavrović-Jankulović, Marija
AU - Đurašinović, Tatjana
AU - Antić, Bratislav
AU - Vranješ-Đurić, Sanja
AU - Stanković, Dalibor
PY - 2021
UR - https://cherry.chem.bg.ac.rs/handle/123456789/4720
AB - S-Adenosyl-L-Homocysteine (AdoHcy) is a crucial pathological factor in homocysteine-associated disorders. We developed an impedimetric approach for single drop S-Adenosyl-L-Homocysteine detection, based on S-Adenosyl-L-Homocysteine hydrolase (SAH), produced in the prokaryotic expression system (E. coli) by recombinant DNA technology, immobilized on citrate acid-coated gallium oxyhydroxide dispersed over single-walled carbon nanotubes (CA/GaO(OH)@SWCNT). The proposed biosensor offers detection of AdoHcy, with a limit of detection (LOD) of 0.17 μM. with operating linear range from 1 to 44 μM. The remarkable stability of gallium oxyhydroxide crystal was further improved by citric acid coating and its optical, electrical and physical anisotropy was skipped using SWCNTs.
PB - Wiley
T2 - Electroanalysis
T1 - S-Adenosyl-L-Homocysteine Hydrolase Immobilized on Citric Acid-capped Gallium Oxyhydroxide on SWCNTs Modified Electrode for AdoHcy Impedimetric Sensing
VL - n/a
IS - n/a
DO - 10.1002/elan.202100362
ER -
@article{
author = "Knežević, Sara and Ognjanović, Miloš and Gavrović-Jankulović, Marija and Đurašinović, Tatjana and Antić, Bratislav and Vranješ-Đurić, Sanja and Stanković, Dalibor",
year = "2021",
abstract = "S-Adenosyl-L-Homocysteine (AdoHcy) is a crucial pathological factor in homocysteine-associated disorders. We developed an impedimetric approach for single drop S-Adenosyl-L-Homocysteine detection, based on S-Adenosyl-L-Homocysteine hydrolase (SAH), produced in the prokaryotic expression system (E. coli) by recombinant DNA technology, immobilized on citrate acid-coated gallium oxyhydroxide dispersed over single-walled carbon nanotubes (CA/GaO(OH)@SWCNT). The proposed biosensor offers detection of AdoHcy, with a limit of detection (LOD) of 0.17 μM. with operating linear range from 1 to 44 μM. The remarkable stability of gallium oxyhydroxide crystal was further improved by citric acid coating and its optical, electrical and physical anisotropy was skipped using SWCNTs.",
publisher = "Wiley",
journal = "Electroanalysis",
title = "S-Adenosyl-L-Homocysteine Hydrolase Immobilized on Citric Acid-capped Gallium Oxyhydroxide on SWCNTs Modified Electrode for AdoHcy Impedimetric Sensing",
volume = "n/a",
number = "n/a",
doi = "10.1002/elan.202100362"
}
Knežević, S., Ognjanović, M., Gavrović-Jankulović, M., Đurašinović, T., Antić, B., Vranješ-Đurić, S.,& Stanković, D.. (2021). S-Adenosyl-L-Homocysteine Hydrolase Immobilized on Citric Acid-capped Gallium Oxyhydroxide on SWCNTs Modified Electrode for AdoHcy Impedimetric Sensing. in Electroanalysis
Wiley., n/a(n/a).
https://doi.org/10.1002/elan.202100362
Knežević S, Ognjanović M, Gavrović-Jankulović M, Đurašinović T, Antić B, Vranješ-Đurić S, Stanković D. S-Adenosyl-L-Homocysteine Hydrolase Immobilized on Citric Acid-capped Gallium Oxyhydroxide on SWCNTs Modified Electrode for AdoHcy Impedimetric Sensing. in Electroanalysis. 2021;n/a(n/a).
doi:10.1002/elan.202100362 .
Knežević, Sara, Ognjanović, Miloš, Gavrović-Jankulović, Marija, Đurašinović, Tatjana, Antić, Bratislav, Vranješ-Đurić, Sanja, Stanković, Dalibor, "S-Adenosyl-L-Homocysteine Hydrolase Immobilized on Citric Acid-capped Gallium Oxyhydroxide on SWCNTs Modified Electrode for AdoHcy Impedimetric Sensing" in Electroanalysis, n/a, no. n/a (2021),
https://doi.org/10.1002/elan.202100362 . .
