Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds
Authors
Ristivojević, Petar
Jovanović, Vesna B.

Milojković-Opsenica, Dušanka

Park, Jihae
Rollinger, Judith M.
Ćirković-Veličković, Tanja

Article (Accepted Version)
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Brown seaweeds are traditionally used as food in Asian countries, and they are a valuable source of bioactive compounds. Herein, a novel high-throughput methodological approach was developed for the tracing of compounds with radical scavenging and antimicrobial activities in Saccharina japonica and Undaria pinnatifida methanol extracts. The seaweed metabolites were separated by a novel high-performance thin-layer chromatography method, the bioactive bands were identified by bioautography assays. The bioactive compounds were characterized with ultra-high-performance liquid chromatography coupled with linear trap quadrupole tandem mass spectrometry. Stearidonic, eicosapentaenoic, and arachidonic acids were identified as major components having radical scavenging and antimicrobial activities. The suggested method provides a fast identification and quantification of bioactive compounds in multicomponent biological samples.
Keywords:
Bioautography / Gas chromatography-mass spectrometry / Planar chromatography / Seaweeds / Ultra-high performance liquid chromatography-mass spectrometrySource:
Food Chemistry, 2021, 334, 127562-Publisher:
- Elsevier
Funding / projects:
- Scholarship Foundation of the Republic of Austria (OeAD) [grant no. ICM-2017-06233].
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-172017)
- Ghent University Global Campus, Belgian Special Research Fund BOF StG No. 01N01718.
- FoodEnTwin-Twinning of research activities for the frontier research in the fields of food, nutrition and environmental omics (EU-810752)
Note:
- This is the peer reviewed version of the following article: (1) Ristivojević, P.; Jovanović, V.; Milojković-Opsenica, D.; Park, J.; Rollinger, J. M.; Ćirković-Veličković, T. Rapid Analytical Approach for Bioprofiling Compounds with Radical Scavenging and Antimicrobial Activities from Seaweeds. Food Chemistry 2021, 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562.
- Supplementary material: https://cherry.chem.bg.ac.rs/handle/123456789/4865
Related info:
- Version of
https://doi.org/10.1016/j.foodchem.2020.127562 - Version of
https://cherry.chem.bg.ac.rs/handle/123456789/4844 - Version of
https://doi.org/10.1016/j.foodchem.2020.127562 - Referenced by
https://cherry.chem.bg.ac.rs/handle/123456789/4865
DOI: 10.1016/j.foodchem.2020.127562
ISSN: 0308-8146
WoS: 000569687200015
Scopus: 2-s2.0-85088227308
URI
http://cherry.chem.bg.ac.rs/handle/123456789/4844http://cherry.chem.bg.ac.rs/handle/123456789/4864
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Ristivojević, Petar AU - Jovanović, Vesna B. AU - Milojković-Opsenica, Dušanka AU - Park, Jihae AU - Rollinger, Judith M. AU - Ćirković-Veličković, Tanja PY - 2021 UR - http://cherry.chem.bg.ac.rs/handle/123456789/4844 UR - http://cherry.chem.bg.ac.rs/handle/123456789/4864 AB - Brown seaweeds are traditionally used as food in Asian countries, and they are a valuable source of bioactive compounds. Herein, a novel high-throughput methodological approach was developed for the tracing of compounds with radical scavenging and antimicrobial activities in Saccharina japonica and Undaria pinnatifida methanol extracts. The seaweed metabolites were separated by a novel high-performance thin-layer chromatography method, the bioactive bands were identified by bioautography assays. The bioactive compounds were characterized with ultra-high-performance liquid chromatography coupled with linear trap quadrupole tandem mass spectrometry. Stearidonic, eicosapentaenoic, and arachidonic acids were identified as major components having radical scavenging and antimicrobial activities. The suggested method provides a fast identification and quantification of bioactive compounds in multicomponent biological samples. PB - Elsevier T2 - Food Chemistry T1 - Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds VL - 334 SP - 127562 DO - 10.1016/j.foodchem.2020.127562 ER -
@article{ author = "Ristivojević, Petar and Jovanović, Vesna B. and Milojković-Opsenica, Dušanka and Park, Jihae and Rollinger, Judith M. and Ćirković-Veličković, Tanja", year = "2021", abstract = "Brown seaweeds are traditionally used as food in Asian countries, and they are a valuable source of bioactive compounds. Herein, a novel high-throughput methodological approach was developed for the tracing of compounds with radical scavenging and antimicrobial activities in Saccharina japonica and Undaria pinnatifida methanol extracts. The seaweed metabolites were separated by a novel high-performance thin-layer chromatography method, the bioactive bands were identified by bioautography assays. The bioactive compounds were characterized with ultra-high-performance liquid chromatography coupled with linear trap quadrupole tandem mass spectrometry. Stearidonic, eicosapentaenoic, and arachidonic acids were identified as major components having radical scavenging and antimicrobial activities. The suggested method provides a fast identification and quantification of bioactive compounds in multicomponent biological samples.", publisher = "Elsevier", journal = "Food Chemistry", title = "Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds", volume = "334", pages = "127562", doi = "10.1016/j.foodchem.2020.127562" }
Ristivojević, P., Jovanović, V. B., Milojković-Opsenica, D., Park, J., Rollinger, J. M.,& Ćirković-Veličković, T.. (2021). Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds. in Food Chemistry Elsevier., 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562
Ristivojević P, Jovanović VB, Milojković-Opsenica D, Park J, Rollinger JM, Ćirković-Veličković T. Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds. in Food Chemistry. 2021;334:127562. doi:10.1016/j.foodchem.2020.127562 .
Ristivojević, Petar, Jovanović, Vesna B., Milojković-Opsenica, Dušanka, Park, Jihae, Rollinger, Judith M., Ćirković-Veličković, Tanja, "Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds" in Food Chemistry, 334 (2021):127562, https://doi.org/10.1016/j.foodchem.2020.127562 . .