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Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer
dc.creator | Krstić-Ristivojević, Maja | |
dc.creator | Grundström, Jeanette | |
dc.creator | Apostolović, Danijela | |
dc.creator | Radomirović, Mirjana Ž. | |
dc.creator | Jovanović, Vesna B. | |
dc.creator | Radoi, Vlad | |
dc.creator | Kiewiet, M. B. Gea | |
dc.creator | Vukojević, Vladana | |
dc.creator | Ćirković-Veličković, Tanja | |
dc.creator | van Hage, Marianne | |
dc.date.accessioned | 2022-01-27T13:51:59Z | |
dc.date.available | 2022-01-27T13:51:59Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://cherry.chem.bg.ac.rs/handle/123456789/4866 | |
dc.description.abstract | Transepithelial transport of proteins is an important step in the immune response to food allergens. Mammalian meat allergy is characterized by an IgE response against the carbohydrate moiety galactosyl-α-1,3-galactose (α-Gal) present on mammalian glycoproteins and glycolipids, which causes severe allergic reactions several hours after red meat consumption. The delayed reaction may be related to the processing of α-Gal carrying proteins in the gastrointestinal tract. The aim of this study was to investigate how protein glycosylation by α-Gal affects the susceptibility to gastric digestion and transport through the Caco-2 cell monolayer. We found that α-Gal glycosylation altered protein susceptibility to gastric digestion, where large protein fragments bearing the α-Gal epitope remained for up to 2 h of digestion. Furthermore, α-Gal glycosylation of the protein hampered transcytosis of the protein through the Caco-2 monolayer. α-Gal epitope on the intact protein could be detected in the endosomal fraction obtained by differential centrifugation of Caco-2 cell lysates. Furthermore, the level of galectin-3 in Caco-2 cells was not affected by the presence of α-Gal glycosylated BSA (bovine serum albumin) (BSA-α-Gal). Taken together, our data add new knowledge and shed light on the digestion and transport of α-Gal glycosylated proteins. | sr |
dc.language.iso | en | sr |
dc.publisher | MDPI | sr |
dc.relation | EAACI Fellowship Award for 2015. | sr |
dc.relation | The Swedish Research Council. | sr |
dc.relation | Region Stockholm (ALF project). | sr |
dc.relation | The Swedish Asthma and Allergy Association’s Research Foundation. | sr |
dc.relation | The King Gustaf V 80th Birthday Foundation. | sr |
dc.relation | The Swedish Heart-Lung Foundation. | sr |
dc.relation | The Hesselman Foundation. | sr |
dc.relation | The Konsul Th C Bergh Foundation. | sr |
dc.relation | The Tore Nilson Foundation for Medical Research. | sr |
dc.relation | The Swedish Cancer and Allergy Foundation. | sr |
dc.relation | The Magnus Bergvall Foundation. | sr |
dc.relation | FP7-HEALTH-2013-INNOVATION-1 Project GLORIA (No. 602919). | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/810752/EU// | sr |
dc.relation | Serbian Academy of Sciences and Arts Project F-26. | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | International Journal of Molecular Sciences | sr |
dc.subject | α-Gal | sr |
dc.subject | transcytosis | sr |
dc.subject | glycoprotein | sr |
dc.subject | glycans | sr |
dc.subject | Caco-2 cells | sr |
dc.subject | mammalian meat allergy | sr |
dc.title | Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer | sr |
dc.type | article | sr |
dc.rights.license | BY | sr |
dc.citation.volume | 21 | |
dc.citation.issue | 16 | |
dc.citation.spage | 5742 | |
dc.identifier.wos | 000565143100001 | |
dc.identifier.doi | 10.3390/ijms21165742 | |
dc.citation.rank | M21 | |
dc.type.version | publishedVersion | sr |
dc.identifier.scopus | 2-s2.0-85089495029 | |
dc.identifier.fulltext | http://cherry.chem.bg.ac.rs/bitstream/id/28872/Alpha-Gal_on_pub_2020.pdf |