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dc.creatorNikolić, Milan
dc.creatorMinić, Simeon L.
dc.creatorMačvanin, Mirjana T.
dc.creatorStanić-Vučinić, Dragana
dc.creatorĆirković-Veličković, Tanja
dc.creatorJacob-Lopes, Eduardo
dc.creatorQueiroz, Maria Isabel
dc.creatorZepka, Leila Queiroz
dc.date.accessioned2022-02-22T09:21:10Z
dc.date.available2022-09-09
dc.date.issued2020
dc.identifier.isbn978-3-030-50971-2
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/4893
dc.description.abstractThe aim of this chapter is to review and discuss methodology and protocols in the analysis of phycobiliproteins (phycocyanins, allophycocyanins, and phycoerythrins) and their chromophores. Due to the presence of multiple covalently bound open-chain tetrapyrrole chromophores, phycobiliproteins are colored and strongly fluorescent molecules, with high absorption coefficients (105 to 106) and excellent fluorescent quantum yield (0.51 up to 0.98). Therefore, a vast number of methods for phycobiliproteins analysis is based on these spectral characteristics, whereas assessment of their bioactivity is related to their exceptional redox and metal-chelating properties. This chapter is dedicated to methods used for isolation and purification, structure analysis, physicochemical properties and stability characterization, quantification, as well as in vitro and in vivo biological activities evaluation. In addition, emerging approaches related to phycobiliproteins analysis are also reviewed including interactions with other biomolecules and ions and identification of phycobiliprotein (chromo)peptides by mass spectrometry.
dc.languageen
dc.publisherSpringer International Publishing
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/810752/EU//
dc.relation.isversionofhttps://cherry.chem.bg.ac.rs/handle/123456789/4892
dc.relation.isversionofhttps://doi.org/10.1007/978-3-030-50971-2_8
dc.rightsembargoedAccess
dc.sourcePigments from Microalgae Handbook
dc.subjectAllophycocyanin
dc.subjectChromopeptides
dc.subjectPhycobiliproteins
dc.subjectPhycocyanin
dc.subjectPhycocyanobilin
dc.subjectPhycoerythrin
dc.titleAnalytical Protocols in Phycobiliproteins Analysis
dc.typebookPart
dc.rights.licenseARR
dc.citation.spage179
dc.citation.epage201
dc.identifier.doi10.1007/978-3-030-50971-2_8
dc.description.otherThis is the peer-reviewed version of the following article: (1) Nikolic, M. R.; Minic, S.; Macvanin, M.; Stanic-Vucinic, D.; Cirkovic Velickovic, T. Analytical Protocols in Phycobiliproteins Analysis. In Pigments from Microalgae Handbook; Jacob-Lopes, E., Queiroz, M. I., Zepka, L. Q., Eds.; Springer International Publishing: Cham, 2020; pp 179–201. [https://doi.org/10.1007/978-3-030-50971-2_8].
dc.type.versionacceptedVersion
dc.identifier.fulltexthttp://cherry.chem.bg.ac.rs/bitstream/id/28960/Analytical_Protocols_in_acc_2020.pdf


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