The influence of the low frequency magnetic field with scan regime from 10 Hz to 50 Hz on Saccharomyces cerevisiae respiration

Abstract

The analysis of the electric, magnetic and electromagnetic fields influence on microorganisms is a very popular research topic, since these fields could potentially act as stressors and affect the microbial metabolism and survival. The aim of this work was to investigate the influence of the low frequency magnetic field (MF) with scan regime from 10 Hz to 50 Hz on S. cerevisiae respiration. The experiment was performed in five replicates and monitored using the Micro-Oxymax® respirometer. All five experiments showed lower cumulative O2 consumption in MF exposed samples, compared to the control sample and inconsistent cumulative CO2 production. However, these differences in O2 consumption and CO2 production were statistically significant. Even though additional experiments are necessary, these results strongly suggest that this is a good basis for further investigation in this field.

Ispitivanje uticaja električnog, magnetnog i elektromagnetnog polja na mikroorganizme jeveoma aktuelni predmet istrazivanja, jer ova fizička polja potencijalno deluju kao faktoristresa i tako utiču na mikrobni metabolizam, ponašanje i preživljavanje. U ovom raduispitivan je uticaj niskofrekventnog magnetnog polja (MP) sa konstantnim intervalomskeniranja od 10 do 50 Hz na respiraciju ćelija kvasca, S. cerevisiae. Eksperiment je rađen upet ponavljanja i praćen Micro-Oxymax® respirometrom. Kumulativna potrošnja kiseonikaje bila manja kod ćelija izloženih MP u svih pet ponavljanja, dok je produkcija CO2 bilanekonzistentna. Međutim, ove razlike u potrošnji O2 i produkciji CO2 su statistički značajne.Iako su dodatna ispitivanja neophodna, dobijeni rezultati ovih inicijalnih eksperimenatapredstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti.