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Effect of α-tocopherol on free thiol content and SOD activity in K562 cells

Efekat α-tokoferola na količinu slobodnih tiola i SOD aktivnost kod K562 ćelija

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2007_leskovac_325.pdf (935.9Kb)
Authors
Spasić, Snežana
Martinov, Olga B.
Bogdanović, Višnja
Miletić, Srđan B.
Vrvić, Miroslav
Article (Published version)
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Abstract
We examined effect of α-tocopherol addition and influence of nitric oxide from sodium nitroprusside (SNP) on free thiol content and SOD activity in exponentially growing K562 cells (human erythroleukaemia) in RPMI 1640 (Sigma) supplemented with 2 mM glutamine, 10 % heat-inactivated fetal calf serum and antibiotics at 37 oC in a humidified 5 % carbon dioxide atmosphere. Cells concentration was of 0.85 x 106 per flask. Our results indicate potential dual role of α-tocopherol: induction of more reduced cell state (increased free -SH) and increased resistance of cancer cells to treatment which induce oxidative stress (increased SOD). α-Tocopherol pretreated K562 cells do not expected respond on the SNP treatment. .
Ispitivali smo efekat dodatka α-tokoferola i uticaj azot-oksida iz natrijum-nitroprusida (NNP) na sadržaj slobodnih tiol grupa i SOD aktivnost kod K562 ćelija (humana eritroleukemija) u eksponencijalnoj fazi rasta, koje su gajene u RPMI 1640 (Sigma) sa dodatkom 2 mM glutamina, 10 % toplotom-inaktiviranog fetalnog govedjeg seruma i antibiotika, na 37 oC u vlažnoj atmosferi sa 5 % ugljen-dioksida. Koncentracija ćelija bila je 0,85 x 106 po posudi. Naši rezultati ukazuju na moguću dvostruku ulogu α-tokoferola kod ovih ćelija: indukciju više redukovanog stanja (porast slobodnih -SH grupa), ali i porast njihove otpornosti na tretmane koji indukuju oksidacioni stres (porast SOD aktivnosti). α-Tokoferolom pretretirane K562 ćelije ne daju očekivani odgovor na tretman sa NNP. .
Source:
Zbornik radova Tehnološkog fakulteta, Leskovac, 2007, 16, 51-56

ISSN: 0352-6542

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Handle
https://hdl.handle.net/21.15107/rcub_cer_325
URI
http://cherry.chem.bg.ac.rs/handle/123456789/4972
Collections
  • Publikacije
Institution/Community
Hemijski fakultet
TY  - JOUR
AU  - Spasić, Snežana
AU  - Martinov, Olga B.
AU  - Bogdanović, Višnja
AU  - Miletić, Srđan B.
AU  - Vrvić, Miroslav
PY  - 2007
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4972
AB  - We examined effect of α-tocopherol addition and influence of nitric oxide from sodium nitroprusside (SNP) on free thiol content and SOD activity in exponentially growing K562 cells (human erythroleukaemia) in RPMI 1640 (Sigma) supplemented with 2 mM glutamine, 10 % heat-inactivated fetal calf serum and antibiotics at 37 oC in a humidified 5 % carbon dioxide atmosphere. Cells concentration was of 0.85 x 106 per flask. Our results indicate potential dual role of α-tocopherol: induction of more reduced cell state (increased free -SH) and increased resistance of cancer cells to treatment which induce oxidative stress (increased SOD). α-Tocopherol pretreated K562 cells do not expected respond on the SNP treatment. .
AB  - Ispitivali smo efekat dodatka α-tokoferola i uticaj azot-oksida iz natrijum-nitroprusida (NNP) na sadržaj slobodnih tiol grupa i SOD aktivnost kod K562 ćelija (humana eritroleukemija) u eksponencijalnoj fazi rasta, koje su gajene u RPMI 1640 (Sigma) sa dodatkom 2 mM glutamina, 10 % toplotom-inaktiviranog fetalnog govedjeg seruma i antibiotika, na 37 oC u vlažnoj atmosferi sa 5 % ugljen-dioksida. Koncentracija ćelija bila je 0,85 x 106 po posudi. Naši rezultati ukazuju na moguću dvostruku ulogu α-tokoferola kod ovih ćelija: indukciju više redukovanog stanja (porast slobodnih -SH grupa), ali i porast njihove otpornosti na tretmane koji indukuju oksidacioni stres (porast SOD aktivnosti). α-Tokoferolom pretretirane K562 ćelije ne daju očekivani odgovor na tretman sa NNP. .
T2  - Zbornik radova Tehnološkog fakulteta, Leskovac
T1  - Effect of α-tocopherol on free thiol content and SOD activity in K562 cells
T1  - Efekat α-tokoferola na količinu slobodnih tiola i SOD aktivnost kod K562 ćelija
IS  - 16
SP  - 51
EP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_cer_325
UR  - Conv_325
ER  - 
@article{
author = "Spasić, Snežana and Martinov, Olga B. and Bogdanović, Višnja and Miletić, Srđan B. and Vrvić, Miroslav",
year = "2007",
abstract = "We examined effect of α-tocopherol addition and influence of nitric oxide from sodium nitroprusside (SNP) on free thiol content and SOD activity in exponentially growing K562 cells (human erythroleukaemia) in RPMI 1640 (Sigma) supplemented with 2 mM glutamine, 10 % heat-inactivated fetal calf serum and antibiotics at 37 oC in a humidified 5 % carbon dioxide atmosphere. Cells concentration was of 0.85 x 106 per flask. Our results indicate potential dual role of α-tocopherol: induction of more reduced cell state (increased free -SH) and increased resistance of cancer cells to treatment which induce oxidative stress (increased SOD). α-Tocopherol pretreated K562 cells do not expected respond on the SNP treatment. ., Ispitivali smo efekat dodatka α-tokoferola i uticaj azot-oksida iz natrijum-nitroprusida (NNP) na sadržaj slobodnih tiol grupa i SOD aktivnost kod K562 ćelija (humana eritroleukemija) u eksponencijalnoj fazi rasta, koje su gajene u RPMI 1640 (Sigma) sa dodatkom 2 mM glutamina, 10 % toplotom-inaktiviranog fetalnog govedjeg seruma i antibiotika, na 37 oC u vlažnoj atmosferi sa 5 % ugljen-dioksida. Koncentracija ćelija bila je 0,85 x 106 po posudi. Naši rezultati ukazuju na moguću dvostruku ulogu α-tokoferola kod ovih ćelija: indukciju više redukovanog stanja (porast slobodnih -SH grupa), ali i porast njihove otpornosti na tretmane koji indukuju oksidacioni stres (porast SOD aktivnosti). α-Tokoferolom pretretirane K562 ćelije ne daju očekivani odgovor na tretman sa NNP. .",
journal = "Zbornik radova Tehnološkog fakulteta, Leskovac",
title = "Effect of α-tocopherol on free thiol content and SOD activity in K562 cells, Efekat α-tokoferola na količinu slobodnih tiola i SOD aktivnost kod K562 ćelija",
number = "16",
pages = "51-56",
url = "https://hdl.handle.net/21.15107/rcub_cer_325, Conv_325"
}
Spasić, S., Martinov, O. B., Bogdanović, V., Miletić, S. B.,& Vrvić, M.. (2007). Effect of α-tocopherol on free thiol content and SOD activity in K562 cells. in Zbornik radova Tehnološkog fakulteta, Leskovac(16), 51-56.
https://hdl.handle.net/21.15107/rcub_cer_325
Spasić S, Martinov OB, Bogdanović V, Miletić SB, Vrvić M. Effect of α-tocopherol on free thiol content and SOD activity in K562 cells. in Zbornik radova Tehnološkog fakulteta, Leskovac. 2007;(16):51-56.
https://hdl.handle.net/21.15107/rcub_cer_325 .
Spasić, Snežana, Martinov, Olga B., Bogdanović, Višnja, Miletić, Srđan B., Vrvić, Miroslav, "Effect of α-tocopherol on free thiol content and SOD activity in K562 cells" in Zbornik radova Tehnološkog fakulteta, Leskovac, no. 16 (2007):51-56,
https://hdl.handle.net/21.15107/rcub_cer_325 .

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