A lectin from the Adriatic sponge Haliclona cratera was purified by ion-exchange and gel chromatography The molecular mass of the lectin is approximately 29 kDa. Purified lectin is rich in hydrophobic and basic amino acids and has an isoelectric point at pH 8.6. H. cratera lectin is relatively heat- and pH-stable. It agglutinates native and trypsinized, papainized and neuraminidase-treated human A, B, O, AB and sheep erythrocytes, and the hemagglutinating activity is independent of Ca2+, Mn2+ and Mg2+ ions; D-galactose and N-acetyl-D-galactosamine are found to be moderate inhibitors of the activity. H. cratera lectin displays cytotoxic effect on HeLa and FemX cells and weak mitogenic effect on human T-lymphocytes pretreated with phytohemagglutinin (PHA). (C) 2002 Elsevier Science Inc. All rights reserved.
TY - JOUR
AU - Pajic, I
AU - Kljajic, Z
AU - Dogovic, N
AU - Sladić, Dušan
AU - Juranic, Z
AU - Gasic, MJ
PY - 2002
UR - http://cherry.chem.bg.ac.rs/handle/123456789/501
AB - A lectin from the Adriatic sponge Haliclona cratera was purified by ion-exchange and gel chromatography The molecular mass of the lectin is approximately 29 kDa. Purified lectin is rich in hydrophobic and basic amino acids and has an isoelectric point at pH 8.6. H. cratera lectin is relatively heat- and pH-stable. It agglutinates native and trypsinized, papainized and neuraminidase-treated human A, B, O, AB and sheep erythrocytes, and the hemagglutinating activity is independent of Ca2+, Mn2+ and Mg2+ ions; D-galactose and N-acetyl-D-galactosamine are found to be moderate inhibitors of the activity. H. cratera lectin displays cytotoxic effect on HeLa and FemX cells and weak mitogenic effect on human T-lymphocytes pretreated with phytohemagglutinin (PHA). (C) 2002 Elsevier Science Inc. All rights reserved.
PB - Elsevier Science Inc, New York
T2 - Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology
T1 - A novel lectin from the sponge Haliclona cratera: isolation, characterization and biological activity
VL - 132
IS - 2
SP - 213
EP - 221
DO - 10.1016/S1532-0456(02)00068-6
ER -
@article{
author = "Pajic, I and Kljajic, Z and Dogovic, N and Sladić, Dušan and Juranic, Z and Gasic, MJ",
year = "2002",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/501",
abstract = "A lectin from the Adriatic sponge Haliclona cratera was purified by ion-exchange and gel chromatography The molecular mass of the lectin is approximately 29 kDa. Purified lectin is rich in hydrophobic and basic amino acids and has an isoelectric point at pH 8.6. H. cratera lectin is relatively heat- and pH-stable. It agglutinates native and trypsinized, papainized and neuraminidase-treated human A, B, O, AB and sheep erythrocytes, and the hemagglutinating activity is independent of Ca2+, Mn2+ and Mg2+ ions; D-galactose and N-acetyl-D-galactosamine are found to be moderate inhibitors of the activity. H. cratera lectin displays cytotoxic effect on HeLa and FemX cells and weak mitogenic effect on human T-lymphocytes pretreated with phytohemagglutinin (PHA). (C) 2002 Elsevier Science Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology",
title = "A novel lectin from the sponge Haliclona cratera: isolation, characterization and biological activity",
volume = "132",
number = "2",
pages = "213-221",
doi = "10.1016/S1532-0456(02)00068-6"
}
Pajic, I., Kljajic, Z., Dogovic, N., Sladić, D., Juranic, Z.,& Gasic, M. (2002). A novel lectin from the sponge Haliclona cratera: isolation, characterization and biological activity.
Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology
Elsevier Science Inc, New York., 132(2), 213-221.
https://doi.org/10.1016/S1532-0456(02)00068-6
Pajic I, Kljajic Z, Dogovic N, Sladić D, Juranic Z, Gasic M. A novel lectin from the sponge Haliclona cratera: isolation, characterization and biological activity. Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology. 2002;132(2):213-221
Pajic I, Kljajic Z, Dogovic N, Sladić Dušan, Juranic Z, Gasic MJ, "A novel lectin from the sponge Haliclona cratera: isolation, characterization and biological activity" Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology, 132, no. 2 (2002):213-221,
https://doi.org/10.1016/S1532-0456(02)00068-6 .
