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dc.creatorĆirković-Veličković, Tanja
dc.creatorGavrović-Jankulović, Marija
dc.creatorPrisic, S
dc.creatorJankov, Ratko M.
dc.creatorBurazer, Lidija M.
dc.creatorVuckovic, O
dc.creatorSporcic, Z
dc.creatorParanos, S
dc.date.accessioned2018-11-22T00:05:01Z
dc.date.available2018-11-22T00:05:01Z
dc.date.issued2002
dc.identifier.issn0105-4538
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/509
dc.description.abstractBackground: Reaction of epsilon-amino groups of lysine with potassium cyanate, maleic, or succinic anhydride leads to allergoids of low molecular weight. No study has been performed to compare their properties and investigate the influence of a residual group on allergenicity and human IgE- and IgG-binding of these derivatives. Methods: Allergoids of a pollen extract of Artemisia vulgaris were obtained by means of potassium cyanate, and succinic and maleic anhydride. Biochemical properties were investigated by determination of amino groups, enzyme activity, isoelectric focusing IEF and SDS-PAGE. IgE- and IgG-binding was determined using immunoblots and ELISA inhibition. Allergenicity was investigated by skin prick tests (SPT) on a group of 52 patients, of which 6 were control subjects, 30 were patients with no previous immunotherapy (IT), and 16 were patients undergoing immunotherapy. Results: The same degree of amino-group modification (more than 85%), residual enzyme activity (less then 15%), IEF, and SDS-PAGE pattern were noted. In the immunoblots of IgE-binding, there was more pronounced reduction in the succinyl and maleyl derivatives than in the carbamyl one. IgG-binding was less affected by carbamylation than by acid anhydride modification. The SPT showed that the succinylated derivative had the most reduced allergenicity (98% showed a reduced wheal diameter when tested with the succinyl derivative, 87% with the maleyl allergoid, and 83% with the carbamyl allergoid). The most significant difference among allergoids could be seen in the group of patients with high skin reactivity (83% of patients showed no reaction to the succinyl derivative when compared to the value of 28% for the carbamyl derivative or 22% for the maleyl derivative). Conclusions: According to our results, all three modification procedures yielded allergoids with a similar extent of modification. No single biochemical parameter investigated in the study could predict the degree of reduced allergenicity in vivo. The most reduced allergenicity was seen in the succinyl derivative while the preservation of IgG binding epitopes was of the highest degree for the carbamyl derivative.en
dc.publisherBlackwell Munksgaard, Copenhagen
dc.rightsrestrictedAccess
dc.sourceAllergy
dc.subjectallergoiden
dc.subjectArtemisia vulgarisen
dc.subjectchemical modificationen
dc.subjectmaleic anhydrideen
dc.subjectmugwort pollenen
dc.subjectpotassium cyanateen
dc.subjectskin prick testingen
dc.subjectsuccinic anhydrideen
dc.titleThe influence of a residual group in low-molecular-weight allergoids of Artemisia vulgaris pollen on their allergenicity, IgE- and IgG-binding propertiesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractСпорциц, З; Ћирковић-Величковић, Тања; Гавровић-Јанкуловић, Марија; Јанков, РМ; Буразер, Л; Вуцковиц, О; Паранос, С; Присиц, С;
dc.citation.volume57
dc.citation.issue11
dc.citation.spage1013
dc.citation.epage1020
dc.identifier.wos000178343400006
dc.identifier.doi10.1034/j.1398-9995.2002.23739.x
dc.citation.other57(11): 1013-1020
dc.citation.rankM21
dc.identifier.pmid12358997
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-0036828313
dc.identifier.rcubKon_1483


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