Show simple item record

dc.creatorFilipović, Lidija
dc.creatorSpasojević, Milica
dc.creatorProdanović, Radivoje
dc.creatorKorać, Aleksandra
dc.creatorMatijaševic, Suzana
dc.creatorBrajušković, Goran
dc.creatorde Marco, Ario
dc.creatorPopović, Milica
dc.date.accessioned2022-05-20T10:59:03Z
dc.date.available2022-05-20T10:59:03Z
dc.date.issued2022
dc.identifier.issn1871-6784
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/5152
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/5153
dc.description.abstractCorrect elucidation of physiological and pathological processes mediated by extracellular vesicles (EV) is highly dependent on the reliability of the method used for their purification. Currently available chemical/physical protocols for sample fractionation are time-consuming, often scarcely reproducible and their yields are low. Immuno-capture based approaches could represent an effective purification alternative to obtain homogeneous EV samples. An easy-to-operate chromatography system was set-up for the purification of intact EVs based on a single domain (VHH) antibodies-copolymer matrix suitable for biological samples as different as conditioned cell culture medium and human plasma. Methacrylate-based copolymer is a porous solid support, the chemical versatility of which enables its efficient functionalization with VHHs. The combined analyses of morphological features and biomarker (CD9, CD63 and CD81) presence indicated that the recovered EVs were exosomes. The lipoprotein markers APO-A1 and APO-B were both negative in tested samples. This is the first report demonstrating the successful application of spherical porous methacrylate-based copolymer coupled with VHHs for the exosome isolation from biological fluids. This inexpensive immunoaffinity method has the potential to be applied for the isolation of EVs belonging to different morphological and physiological classes.
dc.languageEnglish
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200288/RS//
dc.relation.isreferencedbyhttps://doi.org/10.1016/j.nbt.2022.03.001
dc.relation.isreferencedbyhttps://cherry.chem.bg.ac.rs/handle/123456789/5152
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-sa/4.0/
dc.sourceNew Biotechnology
dc.subjectExosomes
dc.subjectExtracellular vesicles
dc.subjectImmunopurification
dc.subjectMethacrylate-based copolymer
dc.subjectNanobodies
dc.titleSupplementary information for the article: Filipović, L.; Spasojević, M.; Prodanović, R.; Korać, A.; Matijaševic, S.; Brajušković, G.; de Marco, A.; Popović, M. Affinity-Based Isolation of Extracellular Vesicles by Means of Single-Domain Antibodies Bound to Macroporous Methacrylate-Based Copolymer. New Biotechnology 2022, 69, 36–48. https://doi.org/10.1016/j.nbt.2022.03.001.
dc.typedataseten
dc.rights.licenseBY-SA
dc.description.otherSupplementary material for: [https://doi.org/10.1016/j.nbt.2022.03.001]
dc.description.otherRelation to published version: [https://cherry.chem.bg.ac.rs/handle/123456789/5152]
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://cherry.chem.bg.ac.rs/bitstream/id/29999/Affinity-based_isolation_sup_2022.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_cherry_5153


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record