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dc.creatorTasic, J
dc.creatorRistić, S
dc.creatorPiperski, V
dc.creatorDacevic, M
dc.creatorPejanovic, V
dc.creatorJokanovic, M
dc.creatorMandić, Ljuba M.
dc.date.accessioned2018-11-22T00:05:08Z
dc.date.available2018-11-22T00:05:08Z
dc.date.issued2002
dc.identifier.issn0731-7085
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/516
dc.description.abstractA rapid and sensitive HPLC-RP method for simultaneous determination of tiazofurin, its 5'-O acetyl and benzoyl esters and their active metabolite thiazole-4-carboxamide adenine dinucleotide was developed and validated. The method allowed determination and quantification of nanomolar quantities of these substances in cell extracts of treated cells, and was also used in kinetic studies of cellular uptake of tiazofurin and its esters from the cultivation medium. Separation of the analyzed substances from unidentified peaks from both biological materials was achieved by gradient elution, thus reducing the possibility of interference. The mobile phase consisted of a 0.1 M sodium-hydrogen phosphate, pH 5.1 and methanol. Run time was 22 min, with 5 min equilibration time. (C) 2002 Elsevier Science B.V. All rights reserved.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.rightsrestrictedAccess
dc.sourceJournal of Pharmaceutical and Biomedical Analysis
dc.subjectHPLCen
dc.subjecttiazofarinen
dc.subjectacetyl and benzoyl estersen
dc.subjectTADen
dc.titleSimultaneous LC determination of tiazofurin, its acetyl and benzoyl esters and their active metabolite thiazole-4-carboxamide adenine dinucleotide in biological samplesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПиперски, В; Јокановиц, М; Мандић, Љуба М.; Пејановиц, В; Дацевиц, М; Ристиц, С; Тасиц, Ј;
dc.citation.volume30
dc.citation.issue4
dc.citation.spage993
dc.citation.epage999
dc.identifier.wos000179506100012
dc.identifier.doi10.1016/S0731-7085(02)00448-X
dc.citation.other30(4): 993-999
dc.citation.rankM22
dc.identifier.pmid12408890
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-0037038352
dc.identifier.rcubKon_1490


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