Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits
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2022
Authors
Krstić, Đurđa D.
Milinčić, Danijel
Kostić, Aleksandar Ž.

Fotirić-Akšić, Milica M.

Stanojević, Slađana P.

Milojković-Opsenica, Dušanka

Pešić, Mirjana
Trifković, Jelena

Article (Published version)

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Show full item recordAbstract
Product authentication is one of the most important food quality assurances. Considering the importance of
consumption of berry fruits with proven health-beneficial properties, high sensory values and rich composition in
bioactive substances, the aim of this study was to evaluate a straightforward and simple procedure for the protein
fingerprinting of berry seeds. For this purpose, protein profiles of 45 samples of genuine berry fruit cultivars
(strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry, and
gojiberry) were analyzed by SDS-PAGE electrophoresis in combination with advanced chemometric tools. The
most important parameters for discrimination among berry seeds were polypeptides at 12.8; 15.1; 25.0; 26.4;
30.0; 41.8; 44.4; 46.0; 48.5; 52.3 and 56.4 kDa. Biomarkers obtained from the protein profile of berry seeds
proved to be a powerful tool in the authentication of their botanical origin, as well as for potential detection o...f
berry-based products adulteration
Keywords:
Berry seeds / Polypeptide profiling / SDS-PAGE electrophoresis / Image analysis / ChemometricsSource:
Food Chemistry, 2022, 383, 132583-Publisher:
- Elsevier
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200168 (University of Belgrade, Faculty of Chemistry) (RS-200168)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200116 (University of Belgrade, Faculty of Agriculture) (RS-200116)
DOI: 10.1016/j.foodchem.2022.132583
ISSN: 0308-8146
WoS: 00077828990001
Scopus: 2-s2.0-85125484658
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Krstić, Đurđa D. AU - Milinčić, Danijel AU - Kostić, Aleksandar Ž. AU - Fotirić-Akšić, Milica M. AU - Stanojević, Slađana P. AU - Milojković-Opsenica, Dušanka AU - Pešić, Mirjana AU - Trifković, Jelena PY - 2022 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5196 AB - Product authentication is one of the most important food quality assurances. Considering the importance of consumption of berry fruits with proven health-beneficial properties, high sensory values and rich composition in bioactive substances, the aim of this study was to evaluate a straightforward and simple procedure for the protein fingerprinting of berry seeds. For this purpose, protein profiles of 45 samples of genuine berry fruit cultivars (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry, and gojiberry) were analyzed by SDS-PAGE electrophoresis in combination with advanced chemometric tools. The most important parameters for discrimination among berry seeds were polypeptides at 12.8; 15.1; 25.0; 26.4; 30.0; 41.8; 44.4; 46.0; 48.5; 52.3 and 56.4 kDa. Biomarkers obtained from the protein profile of berry seeds proved to be a powerful tool in the authentication of their botanical origin, as well as for potential detection of berry-based products adulteration PB - Elsevier T2 - Food Chemistry T1 - Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits VL - 383 SP - 132583 DO - 10.1016/j.foodchem.2022.132583 ER -
@article{ author = "Krstić, Đurđa D. and Milinčić, Danijel and Kostić, Aleksandar Ž. and Fotirić-Akšić, Milica M. and Stanojević, Slađana P. and Milojković-Opsenica, Dušanka and Pešić, Mirjana and Trifković, Jelena", year = "2022", abstract = "Product authentication is one of the most important food quality assurances. Considering the importance of consumption of berry fruits with proven health-beneficial properties, high sensory values and rich composition in bioactive substances, the aim of this study was to evaluate a straightforward and simple procedure for the protein fingerprinting of berry seeds. For this purpose, protein profiles of 45 samples of genuine berry fruit cultivars (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry, and gojiberry) were analyzed by SDS-PAGE electrophoresis in combination with advanced chemometric tools. The most important parameters for discrimination among berry seeds were polypeptides at 12.8; 15.1; 25.0; 26.4; 30.0; 41.8; 44.4; 46.0; 48.5; 52.3 and 56.4 kDa. Biomarkers obtained from the protein profile of berry seeds proved to be a powerful tool in the authentication of their botanical origin, as well as for potential detection of berry-based products adulteration", publisher = "Elsevier", journal = "Food Chemistry", title = "Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits", volume = "383", pages = "132583", doi = "10.1016/j.foodchem.2022.132583" }
Krstić, Đ. D., Milinčić, D., Kostić, A. Ž., Fotirić-Akšić, M. M., Stanojević, S. P., Milojković-Opsenica, D., Pešić, M.,& Trifković, J.. (2022). Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits. in Food Chemistry Elsevier., 383, 132583. https://doi.org/10.1016/j.foodchem.2022.132583
Krstić ĐD, Milinčić D, Kostić AŽ, Fotirić-Akšić MM, Stanojević SP, Milojković-Opsenica D, Pešić M, Trifković J. Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits. in Food Chemistry. 2022;383:132583. doi:10.1016/j.foodchem.2022.132583 .
Krstić, Đurđa D., Milinčić, Danijel, Kostić, Aleksandar Ž., Fotirić-Akšić, Milica M., Stanojević, Slađana P., Milojković-Opsenica, Dušanka, Pešić, Mirjana, Trifković, Jelena, "Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits" in Food Chemistry, 383 (2022):132583, https://doi.org/10.1016/j.foodchem.2022.132583 . .