Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments
Samo za registrovane korisnike
2008
Autori
Pereira, TanyaNikodinović-Runić, Jasmina
Nakazono, Chojin
Dennis, Gary R.
Barrow, Kevin D.
Chuck, Jo-Anne
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Immobilized bacteria are being assessed by industry for drug delivery, novel fermentation systems and the protection of organisms in harsh environments. Alginate bioreactors containing Streptomyces nodosus were examined for community structure, cell viability and amphotericin production under different growth conditions. When cell proliferation was encouraged, substrate hyphae were found inside the alginate matrix and within multicellular projections on the surface of the capsule. The periphery of these projections had erect and branched hyphae, morphologically identical to aerial hyphae. Antibiotic production from immobilized organisms was assessed using conditioned culture medium to eliminate the emergence of a free-dwelling population. These organisms sporulated with reduced antibiotic production compared with free-dwelling cultures. The commitment to sporulate was independent of a surface but dependent on community size and nutritional status. This is the first report of the sporul...ation of S. nodosus in liquid cultures and description of the multicellular community the organism adopts at a solid-liquid interface.
Izvor:
Microbial Biotechnology, 2008, 1, 5, 373-381Izdavač:
- Wiley-Blackwell, Malden
DOI: 10.1111/j.1751-7915.2008.00032.x
ISSN: 1751-7907
PubMed: 21261857
WoS: 000207904700006
Scopus: 2-s2.0-77953565330
Kolekcije
Institucija/grupa
Inovacioni centar / Innovation CentreTY - JOUR AU - Pereira, Tanya AU - Nikodinović-Runić, Jasmina AU - Nakazono, Chojin AU - Dennis, Gary R. AU - Barrow, Kevin D. AU - Chuck, Jo-Anne PY - 2008 UR - https://cherry.chem.bg.ac.rs/handle/123456789/525 AB - Immobilized bacteria are being assessed by industry for drug delivery, novel fermentation systems and the protection of organisms in harsh environments. Alginate bioreactors containing Streptomyces nodosus were examined for community structure, cell viability and amphotericin production under different growth conditions. When cell proliferation was encouraged, substrate hyphae were found inside the alginate matrix and within multicellular projections on the surface of the capsule. The periphery of these projections had erect and branched hyphae, morphologically identical to aerial hyphae. Antibiotic production from immobilized organisms was assessed using conditioned culture medium to eliminate the emergence of a free-dwelling population. These organisms sporulated with reduced antibiotic production compared with free-dwelling cultures. The commitment to sporulate was independent of a surface but dependent on community size and nutritional status. This is the first report of the sporulation of S. nodosus in liquid cultures and description of the multicellular community the organism adopts at a solid-liquid interface. PB - Wiley-Blackwell, Malden T2 - Microbial Biotechnology T1 - Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments VL - 1 IS - 5 SP - 373 EP - 381 DO - 10.1111/j.1751-7915.2008.00032.x ER -
@article{ author = "Pereira, Tanya and Nikodinović-Runić, Jasmina and Nakazono, Chojin and Dennis, Gary R. and Barrow, Kevin D. and Chuck, Jo-Anne", year = "2008", abstract = "Immobilized bacteria are being assessed by industry for drug delivery, novel fermentation systems and the protection of organisms in harsh environments. Alginate bioreactors containing Streptomyces nodosus were examined for community structure, cell viability and amphotericin production under different growth conditions. When cell proliferation was encouraged, substrate hyphae were found inside the alginate matrix and within multicellular projections on the surface of the capsule. The periphery of these projections had erect and branched hyphae, morphologically identical to aerial hyphae. Antibiotic production from immobilized organisms was assessed using conditioned culture medium to eliminate the emergence of a free-dwelling population. These organisms sporulated with reduced antibiotic production compared with free-dwelling cultures. The commitment to sporulate was independent of a surface but dependent on community size and nutritional status. This is the first report of the sporulation of S. nodosus in liquid cultures and description of the multicellular community the organism adopts at a solid-liquid interface.", publisher = "Wiley-Blackwell, Malden", journal = "Microbial Biotechnology", title = "Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments", volume = "1", number = "5", pages = "373-381", doi = "10.1111/j.1751-7915.2008.00032.x" }
Pereira, T., Nikodinović-Runić, J., Nakazono, C., Dennis, G. R., Barrow, K. D.,& Chuck, J.. (2008). Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments. in Microbial Biotechnology Wiley-Blackwell, Malden., 1(5), 373-381. https://doi.org/10.1111/j.1751-7915.2008.00032.x
Pereira T, Nikodinović-Runić J, Nakazono C, Dennis GR, Barrow KD, Chuck J. Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments. in Microbial Biotechnology. 2008;1(5):373-381. doi:10.1111/j.1751-7915.2008.00032.x .
Pereira, Tanya, Nikodinović-Runić, Jasmina, Nakazono, Chojin, Dennis, Gary R., Barrow, Kevin D., Chuck, Jo-Anne, "Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments" in Microbial Biotechnology, 1, no. 5 (2008):373-381, https://doi.org/10.1111/j.1751-7915.2008.00032.x . .