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Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare

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2021
bitstream_30318.pdf (153.4Kb)
Authors
Trifković, Jelena
Jović, Marko D.
Živković-Radovanović, Vukosava
Andrić, Filip
Ristivojević, Petar
Milojković-Opsenica, Dušanka
Conference object (Published version)
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Abstract
The guarantee of food safety and quality along the food chain has become an important issue which challenges the worldwide and attracts the extensive attention. In order to ensure food safety and quality, efficient analytical methodologies must be applied in the detection of food authenticity, origin, product quality control, food microbiology, genetically modified ingredients, pesticide residues, allergens and toxins.[1] The aim of this study was to develop a green, simple, and high throughput procedure for the quality assessment of food supplements using a high-performance thin-layer chromatography (HPTLC)/bioautography with multivariate statistics. Bioautography assays were developed for the identification of constituents with radical scavenging (DPPH assay), antimicrobial (Bacillus subtilis, Escherichia coli, Micrococus luteus), and enzymes (alpha-amylase) activities of different oregano samples (Figure 1). Further, the combination of chemical screening/bioprofiling and chemometric...s was used for identification of target, most potent compounds and additionally, search of the most potent oregano samples. Due to several advantages such as minimum requirements for sample preparation and minimal amount of investigated sample, possibility of selection of eco-friendly and harmless organic solvents, in situ identification of target compounds, multi detection of investigated compounds, and low solvent consumption (200-500 μL per sample) planar chromatography/bioautography is recognized as green technique. Methodology suggested in this study confirmed its possible application in quality control of food supplements.

Source:
27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia, 2021
Funding / projects:
  • FoodEnTwin-Twinning of research activities for the frontier research in the fields of food, nutrition and environmental omics (EU-810752)
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200168 (University of Belgrade, Faculty of Chemistry) (RS-200168)
Note:
  • Poster: https://cherry.chem.bg.ac.rs/handle/123456789/5229
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_cherry_5263
URI
http://cherry.chem.bg.ac.rs/handle/123456789/5263
Collections
  • Publikacije
Institution/Community
Hemijski fakultet
TY  - CONF
AU  - Trifković, Jelena
AU  - Jović, Marko D.
AU  - Živković-Radovanović, Vukosava
AU  - Andrić, Filip
AU  - Ristivojević, Petar
AU  - Milojković-Opsenica, Dušanka
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5263
AB  - The guarantee of food safety and quality along the food chain has become an important issue which challenges the worldwide and attracts the extensive attention. In order to ensure food safety and quality, efficient analytical methodologies must be applied in the detection of food authenticity, origin, product quality control, food microbiology, genetically modified ingredients, pesticide residues, allergens and toxins.[1] The aim of this study was to develop a green, simple, and high throughput procedure for the quality assessment of food supplements using a high-performance thin-layer chromatography (HPTLC)/bioautography with multivariate statistics. Bioautography assays were developed for the identification of constituents with radical scavenging (DPPH assay), antimicrobial (Bacillus subtilis, Escherichia coli, Micrococus luteus), and enzymes (alpha-amylase) activities of different oregano samples (Figure 1). Further, the combination of chemical screening/bioprofiling and chemometrics was used for identification of target, most potent compounds and additionally, search of the most potent oregano samples. Due to several advantages such as minimum requirements for sample preparation and minimal amount of investigated sample, possibility of selection of eco-friendly and harmless organic solvents, in situ identification of target compounds, multi detection of investigated compounds, and low solvent consumption (200-500 μL per sample) planar chromatography/bioautography is recognized as green technique. Methodology suggested in this study confirmed its possible application in quality control of food supplements.
C3  - 27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia
T1  - Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5263
ER  - 
@conference{
author = "Trifković, Jelena and Jović, Marko D. and Živković-Radovanović, Vukosava and Andrić, Filip and Ristivojević, Petar and Milojković-Opsenica, Dušanka",
year = "2021",
abstract = "The guarantee of food safety and quality along the food chain has become an important issue which challenges the worldwide and attracts the extensive attention. In order to ensure food safety and quality, efficient analytical methodologies must be applied in the detection of food authenticity, origin, product quality control, food microbiology, genetically modified ingredients, pesticide residues, allergens and toxins.[1] The aim of this study was to develop a green, simple, and high throughput procedure for the quality assessment of food supplements using a high-performance thin-layer chromatography (HPTLC)/bioautography with multivariate statistics. Bioautography assays were developed for the identification of constituents with radical scavenging (DPPH assay), antimicrobial (Bacillus subtilis, Escherichia coli, Micrococus luteus), and enzymes (alpha-amylase) activities of different oregano samples (Figure 1). Further, the combination of chemical screening/bioprofiling and chemometrics was used for identification of target, most potent compounds and additionally, search of the most potent oregano samples. Due to several advantages such as minimum requirements for sample preparation and minimal amount of investigated sample, possibility of selection of eco-friendly and harmless organic solvents, in situ identification of target compounds, multi detection of investigated compounds, and low solvent consumption (200-500 μL per sample) planar chromatography/bioautography is recognized as green technique. Methodology suggested in this study confirmed its possible application in quality control of food supplements.",
journal = "27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia",
title = "Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5263"
}
Trifković, J., Jović, M. D., Živković-Radovanović, V., Andrić, F., Ristivojević, P.,& Milojković-Opsenica, D.. (2021). Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare. in 27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia.
https://hdl.handle.net/21.15107/rcub_cherry_5263
Trifković J, Jović MD, Živković-Radovanović V, Andrić F, Ristivojević P, Milojković-Opsenica D. Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare. in 27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_5263 .
Trifković, Jelena, Jović, Marko D., Živković-Radovanović, Vukosava, Andrić, Filip, Ristivojević, Petar, Milojković-Opsenica, Dušanka, "Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare" in 27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia (2021),
https://hdl.handle.net/21.15107/rcub_cherry_5263 .

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