Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study
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2022
Authors
Bartolić, DraganaMojović, Miloš

Prokopijević, Miloš

Đikanović, Daniela
Kalauzi, Aleksandar

Mutavdžić, Dragosav

Baošić, Rada

Radotić, Ksenija

Article (Published version)

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Aflatoxin B1 (AFB1), is a naturally hazardous environmental contaminant, contained mainly in various cereal seeds. The maize (Zea mays L.) seeds containing various levels of the AFB1 was investigated using optical and electron paramagnetic resonance spectroscopies. The changes of lignin content and organic free radicals as indicators of the seeds' defense response to the AFB1-stressor were estimated, in the seeds' inner and outer fractions. The lignin content was determined using the Acetyl Bromide method. No correlation was found between the lignin content and the AFB1 concentration in the inner fraction (R of 0.030, p = 0.943), while a significant positive linear correlation (R of 0.9923, p= 0.00005) between the lignin content and the AFB1 concentration in the outer fraction was obtained. Employing electron paramagnetic resonance (EPR) spectroscopy, the different response of the fractions was observed, regarding the content of free radicals with increasing AFB1 concentrations. In add...ition, front-face fluorescence spectroscopy in combination with the deconvolution showed a positive linear correlation between the ratio of the green and blue spectral emission components (C4/C2) area and the AFB1 concentration in the outer fraction. Thus lignin content and the (C4/C2) area ratio may be reliable indicators for the screening of lignin changes related to the level of AFB1 in the seeds.
Keywords:
Maize (Zea mays L.) seeds / AFB1 / Lignin content / Free radicals / EPR spectroscopy / Fluorescence spectroscopy / food contaminantSource:
Journal of the Science of Food and Agriculture, 2022, 102, 6, 2500-2505Publisher:
- Wiley
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200168 (University of Belgrade, Faculty of Chemistry) (RS-200168)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200146 (University of Belgrade, Faculty of Physical Chemistry) (RS-200146)
DOI: 10.1002/jsfa.11591
ISSN: 0022-5142
WoS: 000716540500001
Scopus: 2-s2.0-85118771838
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Bartolić, Dragana AU - Mojović, Miloš AU - Prokopijević, Miloš AU - Đikanović, Daniela AU - Kalauzi, Aleksandar AU - Mutavdžić, Dragosav AU - Baošić, Rada AU - Radotić, Ksenija PY - 2022 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5268 AB - Aflatoxin B1 (AFB1), is a naturally hazardous environmental contaminant, contained mainly in various cereal seeds. The maize (Zea mays L.) seeds containing various levels of the AFB1 was investigated using optical and electron paramagnetic resonance spectroscopies. The changes of lignin content and organic free radicals as indicators of the seeds' defense response to the AFB1-stressor were estimated, in the seeds' inner and outer fractions. The lignin content was determined using the Acetyl Bromide method. No correlation was found between the lignin content and the AFB1 concentration in the inner fraction (R of 0.030, p = 0.943), while a significant positive linear correlation (R of 0.9923, p= 0.00005) between the lignin content and the AFB1 concentration in the outer fraction was obtained. Employing electron paramagnetic resonance (EPR) spectroscopy, the different response of the fractions was observed, regarding the content of free radicals with increasing AFB1 concentrations. In addition, front-face fluorescence spectroscopy in combination with the deconvolution showed a positive linear correlation between the ratio of the green and blue spectral emission components (C4/C2) area and the AFB1 concentration in the outer fraction. Thus lignin content and the (C4/C2) area ratio may be reliable indicators for the screening of lignin changes related to the level of AFB1 in the seeds. PB - Wiley T2 - Journal of the Science of Food and Agriculture T1 - Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study VL - 102 IS - 6 SP - 2500 EP - 2505 DO - 10.1002/jsfa.11591 ER -
@article{ author = "Bartolić, Dragana and Mojović, Miloš and Prokopijević, Miloš and Đikanović, Daniela and Kalauzi, Aleksandar and Mutavdžić, Dragosav and Baošić, Rada and Radotić, Ksenija", year = "2022", abstract = "Aflatoxin B1 (AFB1), is a naturally hazardous environmental contaminant, contained mainly in various cereal seeds. The maize (Zea mays L.) seeds containing various levels of the AFB1 was investigated using optical and electron paramagnetic resonance spectroscopies. The changes of lignin content and organic free radicals as indicators of the seeds' defense response to the AFB1-stressor were estimated, in the seeds' inner and outer fractions. The lignin content was determined using the Acetyl Bromide method. No correlation was found between the lignin content and the AFB1 concentration in the inner fraction (R of 0.030, p = 0.943), while a significant positive linear correlation (R of 0.9923, p= 0.00005) between the lignin content and the AFB1 concentration in the outer fraction was obtained. Employing electron paramagnetic resonance (EPR) spectroscopy, the different response of the fractions was observed, regarding the content of free radicals with increasing AFB1 concentrations. In addition, front-face fluorescence spectroscopy in combination with the deconvolution showed a positive linear correlation between the ratio of the green and blue spectral emission components (C4/C2) area and the AFB1 concentration in the outer fraction. Thus lignin content and the (C4/C2) area ratio may be reliable indicators for the screening of lignin changes related to the level of AFB1 in the seeds.", publisher = "Wiley", journal = "Journal of the Science of Food and Agriculture", title = "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study", volume = "102", number = "6", pages = "2500-2505", doi = "10.1002/jsfa.11591" }
Bartolić, D., Mojović, M., Prokopijević, M., Đikanović, D., Kalauzi, A., Mutavdžić, D., Baošić, R.,& Radotić, K.. (2022). Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture Wiley., 102(6), 2500-2505. https://doi.org/10.1002/jsfa.11591
Bartolić D, Mojović M, Prokopijević M, Đikanović D, Kalauzi A, Mutavdžić D, Baošić R, Radotić K. Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture. 2022;102(6):2500-2505. doi:10.1002/jsfa.11591 .
Bartolić, Dragana, Mojović, Miloš, Prokopijević, Miloš, Đikanović, Daniela, Kalauzi, Aleksandar, Mutavdžić, Dragosav, Baošić, Rada, Radotić, Ksenija, "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study" in Journal of the Science of Food and Agriculture, 102, no. 6 (2022):2500-2505, https://doi.org/10.1002/jsfa.11591 . .