Influence of pigments and pH of urine on the determination of N-acetyl-beta-D-glucosaminidase activity with 2-methoxy-4-(2 '-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide
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2005
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Prikaz svih podataka o dokumentuApstrakt
The influence of urinary pigments and urine pH on the spectrophotometric determination of N-acetyl-beta-D-glucosaminidase (NAG; EC 3.2.1.30) activity with 2-methoxy-4-(2'-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide as a substrate was studied. The investigation was performed with human and rabbit urine samples. It was found that alkaline urine pH values influenced NAG activity in two ways: 1) NAG activity decreased due to enzyme instability with pH increase, and 2) NAG activity increased because of the contribution of urinary pigments to absorbance of 2-methoxy-4-(2'-nitrovinyl)-phenol (MNP) at 505nm. It was shown that besides the maximum (1) in the range of 350-360nm of the absorption spectra of alkaline urine, there was a maximum (II) in the range of 380-460nm. With the increase of pH, maximum 11 was shifted toward higher wavelengths and contributed to MNP absorption (5-90%). On the other hand, the maximum of MNP absorption was shifted toward lower wavelengths (495-400nm) with in...creasing pH. Two procedures to eliminate the influence of urinary pigments are presented. The justification of applying a correction to the values of NAG activity in human and rabbit urine (a model system for studying the toxic effects of cadmium) was discussed.
Ključne reči:
alkaline urine / urinary pigments / N-acetyl-beta-D-glucosaminidase (NAG) spectrophotometric determinationIzvor:
Journal of Clinical Laboratory Analysis, 2005, 19, 6, 260-266Izdavač:
- Wiley-Liss, Hoboken
DOI: 10.1002/jcla.20088
ISSN: 0887-8013
PubMed: 16302206
WoS: 000233701100006
Scopus: 2-s2.0-28444445592
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Aćimović, Jelena M. AU - Jovanović, Vesna B. AU - Mandić, Ljuba M. PY - 2005 UR - https://cherry.chem.bg.ac.rs/handle/123456789/527 AB - The influence of urinary pigments and urine pH on the spectrophotometric determination of N-acetyl-beta-D-glucosaminidase (NAG; EC 3.2.1.30) activity with 2-methoxy-4-(2'-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide as a substrate was studied. The investigation was performed with human and rabbit urine samples. It was found that alkaline urine pH values influenced NAG activity in two ways: 1) NAG activity decreased due to enzyme instability with pH increase, and 2) NAG activity increased because of the contribution of urinary pigments to absorbance of 2-methoxy-4-(2'-nitrovinyl)-phenol (MNP) at 505nm. It was shown that besides the maximum (1) in the range of 350-360nm of the absorption spectra of alkaline urine, there was a maximum (II) in the range of 380-460nm. With the increase of pH, maximum 11 was shifted toward higher wavelengths and contributed to MNP absorption (5-90%). On the other hand, the maximum of MNP absorption was shifted toward lower wavelengths (495-400nm) with increasing pH. Two procedures to eliminate the influence of urinary pigments are presented. The justification of applying a correction to the values of NAG activity in human and rabbit urine (a model system for studying the toxic effects of cadmium) was discussed. PB - Wiley-Liss, Hoboken T2 - Journal of Clinical Laboratory Analysis T1 - Influence of pigments and pH of urine on the determination of N-acetyl-beta-D-glucosaminidase activity with 2-methoxy-4-(2 '-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide VL - 19 IS - 6 SP - 260 EP - 266 DO - 10.1002/jcla.20088 ER -
@article{ author = "Aćimović, Jelena M. and Jovanović, Vesna B. and Mandić, Ljuba M.", year = "2005", abstract = "The influence of urinary pigments and urine pH on the spectrophotometric determination of N-acetyl-beta-D-glucosaminidase (NAG; EC 3.2.1.30) activity with 2-methoxy-4-(2'-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide as a substrate was studied. The investigation was performed with human and rabbit urine samples. It was found that alkaline urine pH values influenced NAG activity in two ways: 1) NAG activity decreased due to enzyme instability with pH increase, and 2) NAG activity increased because of the contribution of urinary pigments to absorbance of 2-methoxy-4-(2'-nitrovinyl)-phenol (MNP) at 505nm. It was shown that besides the maximum (1) in the range of 350-360nm of the absorption spectra of alkaline urine, there was a maximum (II) in the range of 380-460nm. With the increase of pH, maximum 11 was shifted toward higher wavelengths and contributed to MNP absorption (5-90%). On the other hand, the maximum of MNP absorption was shifted toward lower wavelengths (495-400nm) with increasing pH. Two procedures to eliminate the influence of urinary pigments are presented. The justification of applying a correction to the values of NAG activity in human and rabbit urine (a model system for studying the toxic effects of cadmium) was discussed.", publisher = "Wiley-Liss, Hoboken", journal = "Journal of Clinical Laboratory Analysis", title = "Influence of pigments and pH of urine on the determination of N-acetyl-beta-D-glucosaminidase activity with 2-methoxy-4-(2 '-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide", volume = "19", number = "6", pages = "260-266", doi = "10.1002/jcla.20088" }
Aćimović, J. M., Jovanović, V. B.,& Mandić, L. M.. (2005). Influence of pigments and pH of urine on the determination of N-acetyl-beta-D-glucosaminidase activity with 2-methoxy-4-(2 '-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide. in Journal of Clinical Laboratory Analysis Wiley-Liss, Hoboken., 19(6), 260-266. https://doi.org/10.1002/jcla.20088
Aćimović JM, Jovanović VB, Mandić LM. Influence of pigments and pH of urine on the determination of N-acetyl-beta-D-glucosaminidase activity with 2-methoxy-4-(2 '-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide. in Journal of Clinical Laboratory Analysis. 2005;19(6):260-266. doi:10.1002/jcla.20088 .
Aćimović, Jelena M., Jovanović, Vesna B., Mandić, Ljuba M., "Influence of pigments and pH of urine on the determination of N-acetyl-beta-D-glucosaminidase activity with 2-methoxy-4-(2 '-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide" in Journal of Clinical Laboratory Analysis, 19, no. 6 (2005):260-266, https://doi.org/10.1002/jcla.20088 . .