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dc.creatorAćimović, Jelena M.
dc.creatorJovanović, Vesna B.
dc.creatorMandić, Ljuba M.
dc.date.accessioned2018-11-22T00:08:00Z
dc.date.available2018-11-22T00:08:00Z
dc.date.issued2005
dc.identifier.issn0887-8013
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/527
dc.description.abstractThe influence of urinary pigments and urine pH on the spectrophotometric determination of N-acetyl-beta-D-glucosaminidase (NAG; EC 3.2.1.30) activity with 2-methoxy-4-(2'-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide as a substrate was studied. The investigation was performed with human and rabbit urine samples. It was found that alkaline urine pH values influenced NAG activity in two ways: 1) NAG activity decreased due to enzyme instability with pH increase, and 2) NAG activity increased because of the contribution of urinary pigments to absorbance of 2-methoxy-4-(2'-nitrovinyl)-phenol (MNP) at 505nm. It was shown that besides the maximum (1) in the range of 350-360nm of the absorption spectra of alkaline urine, there was a maximum (II) in the range of 380-460nm. With the increase of pH, maximum 11 was shifted toward higher wavelengths and contributed to MNP absorption (5-90%). On the other hand, the maximum of MNP absorption was shifted toward lower wavelengths (495-400nm) with increasing pH. Two procedures to eliminate the influence of urinary pigments are presented. The justification of applying a correction to the values of NAG activity in human and rabbit urine (a model system for studying the toxic effects of cadmium) was discussed.en
dc.publisherWiley-Liss, Hoboken
dc.rightsrestrictedAccess
dc.sourceJournal of Clinical Laboratory Analysis
dc.subjectalkaline urineen
dc.subjecturinary pigmentsen
dc.subjectN-acetyl-beta-D-glucosaminidase (NAG) spectrophotometric determinationen
dc.titleInfluence of pigments and pH of urine on the determination of N-acetyl-beta-D-glucosaminidase activity with 2-methoxy-4-(2 '-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminideen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМандић, Љуба М.; Јовановић, Весна; Aћимовић, Јелена;
dc.citation.volume19
dc.citation.issue6
dc.citation.spage260
dc.citation.epage266
dc.identifier.wos000233701100006
dc.identifier.doi10.1002/jcla.20888
dc.citation.other19(6): 260-266
dc.citation.rankM23
dc.identifier.pmid16302206
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-28444445592
dc.identifier.rcubKon_1501


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