dc.creator | Aćimović, Jelena M. | |
dc.creator | Jovanović, Vesna B. | |
dc.creator | Mandić, Ljuba M. | |
dc.date.accessioned | 2018-11-22T00:08:00Z | |
dc.date.available | 2018-11-22T00:08:00Z | |
dc.date.issued | 2005 | |
dc.identifier.issn | 0887-8013 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/527 | |
dc.description.abstract | The influence of urinary pigments and urine pH on the spectrophotometric determination of N-acetyl-beta-D-glucosaminidase (NAG; EC 3.2.1.30) activity with 2-methoxy-4-(2'-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide as a substrate was studied. The investigation was performed with human and rabbit urine samples. It was found that alkaline urine pH values influenced NAG activity in two ways: 1) NAG activity decreased due to enzyme instability with pH increase, and 2) NAG activity increased because of the contribution of urinary pigments to absorbance of 2-methoxy-4-(2'-nitrovinyl)-phenol (MNP) at 505nm. It was shown that besides the maximum (1) in the range of 350-360nm of the absorption spectra of alkaline urine, there was a maximum (II) in the range of 380-460nm. With the increase of pH, maximum 11 was shifted toward higher wavelengths and contributed to MNP absorption (5-90%). On the other hand, the maximum of MNP absorption was shifted toward lower wavelengths (495-400nm) with increasing pH. Two procedures to eliminate the influence of urinary pigments are presented. The justification of applying a correction to the values of NAG activity in human and rabbit urine (a model system for studying the toxic effects of cadmium) was discussed. | en |
dc.publisher | Wiley-Liss, Hoboken | |
dc.rights | restrictedAccess | |
dc.source | Journal of Clinical Laboratory Analysis | |
dc.subject | alkaline urine | en |
dc.subject | urinary pigments | en |
dc.subject | N-acetyl-beta-D-glucosaminidase (NAG) spectrophotometric determination | en |
dc.title | Influence of pigments and pH of urine on the determination of N-acetyl-beta-D-glucosaminidase activity with 2-methoxy-4-(2 '-nitrovinyl)-phenyl-N-acetyl-beta-D-glucosaminide | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Мандић, Љуба М.; Јовановић, Весна; Aћимовић, Јелена; | |
dc.citation.volume | 19 | |
dc.citation.issue | 6 | |
dc.citation.spage | 260 | |
dc.citation.epage | 266 | |
dc.identifier.wos | 000233701100006 | |
dc.identifier.doi | 10.1002/jcla.20088 | |
dc.citation.other | 19(6): 260-266 | |
dc.citation.rank | M23 | |
dc.identifier.pmid | 16302206 | |
dc.type.version | publishedVersion | en |
dc.identifier.scopus | 2-s2.0-28444445592 | |