Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes
Imobilizacija Na,K-ATPpaze izolovane iz sinaptičkih plazma-membrana mozga pacova
Apstrakt
Rat brain Na,K-ATPase partially purified by SDS from synaptic plasma membranes (SPM) was immobilized by adsorption on nitrocellulose (NC), polyvinylidene fluoride (PVDF) and glass fiber (GF) membranes, Partial SDS solubilization increased the enzyme activity by 40%. With regard to the presentation of the enzyme activity. nitrocellulose Was shown to be the optimal support for die immobilization, The enzyme showed the highest percentage activity (14%) after 30 min of SPM adsorption. at 20 degreesC under the vaccum. with 25 mug of proteins per NC disc filter. In addition, adsorption on NC stabilizes the Na,K-ATPase, since the activity was substantial 72 h after adsorption at 20 degreesC, After adsorption. the sensitivity of the enzyme to HgCl2 and CdCl2 inhibition was higher, The results show that immobilized Na,K-NTPase SPM can be used as a practical model for the detection of metal ions in different samples.
Delimično prečišćena Na,K-ATPaza sinaptičkih plazma–membrana (SPM) mozga pacova imobilizovana je adsorpcijom na nitrocelulozne (NC) poliviniliden-fluorid (PVDF) membrane i membrane od staklenih vlakana (SV). Aktivnost enzima delimično prečišćenog solubilizacijom SDS-om povećana je oko 40%. Najveći procenat aktivnosti (14%) enzim zadržava posle 30 minuta adsorpcije SPM na 20ºC, pod vakuumom, sa 25 μg proteina po nitroceluloznom disku. Na,K-ATPaza imobilizovana na nitroceluloznoj membrani stabilna je 72 sata na 20ºC. Adsorpcijom, osetljivost enzima na inhibiciju Hg2+ i Cd2+ se povećava. Rezultati pokazuju da se imobilizovana Na,K-ATPaza SPM može koristiti za detekciju toksičnih metalnih jona u različitim uzorcima.
Ključne reči:
Na,K-ATPase / synaptic plasma membranes / synaptic plasma membranes / immobilization / immobilization / inhibition / inhibition / cadmium / cadmium / mercury / mercuryIzvor:
Journal of the Serbian Chemical Society, 2002, 67, 12, 809-817Izdavač:
- Serbian Chemical Soc, Belgrade
DOI: 10.2298/JSC0212809M
ISSN: 0352-5139
WoS: 000180192500003
Scopus: 2-s2.0-0036981182
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Momic, T AU - Vujčić, Zoran AU - Vasic, V AU - Horvat, A PY - 2002 UR - https://cherry.chem.bg.ac.rs/handle/123456789/530 AB - Rat brain Na,K-ATPase partially purified by SDS from synaptic plasma membranes (SPM) was immobilized by adsorption on nitrocellulose (NC), polyvinylidene fluoride (PVDF) and glass fiber (GF) membranes, Partial SDS solubilization increased the enzyme activity by 40%. With regard to the presentation of the enzyme activity. nitrocellulose Was shown to be the optimal support for die immobilization, The enzyme showed the highest percentage activity (14%) after 30 min of SPM adsorption. at 20 degreesC under the vaccum. with 25 mug of proteins per NC disc filter. In addition, adsorption on NC stabilizes the Na,K-ATPase, since the activity was substantial 72 h after adsorption at 20 degreesC, After adsorption. the sensitivity of the enzyme to HgCl2 and CdCl2 inhibition was higher, The results show that immobilized Na,K-NTPase SPM can be used as a practical model for the detection of metal ions in different samples. AB - Delimično prečišćena Na,K-ATPaza sinaptičkih plazma–membrana (SPM) mozga pacova imobilizovana je adsorpcijom na nitrocelulozne (NC) poliviniliden-fluorid (PVDF) membrane i membrane od staklenih vlakana (SV). Aktivnost enzima delimično prečišćenog solubilizacijom SDS-om povećana je oko 40%. Najveći procenat aktivnosti (14%) enzim zadržava posle 30 minuta adsorpcije SPM na 20ºC, pod vakuumom, sa 25 μg proteina po nitroceluloznom disku. Na,K-ATPaza imobilizovana na nitroceluloznoj membrani stabilna je 72 sata na 20ºC. Adsorpcijom, osetljivost enzima na inhibiciju Hg2+ i Cd2+ se povećava. Rezultati pokazuju da se imobilizovana Na,K-ATPaza SPM može koristiti za detekciju toksičnih metalnih jona u različitim uzorcima. PB - Serbian Chemical Soc, Belgrade T2 - Journal of the Serbian Chemical Society T1 - Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes T1 - Imobilizacija Na,K-ATPpaze izolovane iz sinaptičkih plazma-membrana mozga pacova VL - 67 IS - 12 SP - 809 EP - 817 DO - 10.2298/JSC0212809M ER -
@article{ author = "Momic, T and Vujčić, Zoran and Vasic, V and Horvat, A", year = "2002", abstract = "Rat brain Na,K-ATPase partially purified by SDS from synaptic plasma membranes (SPM) was immobilized by adsorption on nitrocellulose (NC), polyvinylidene fluoride (PVDF) and glass fiber (GF) membranes, Partial SDS solubilization increased the enzyme activity by 40%. With regard to the presentation of the enzyme activity. nitrocellulose Was shown to be the optimal support for die immobilization, The enzyme showed the highest percentage activity (14%) after 30 min of SPM adsorption. at 20 degreesC under the vaccum. with 25 mug of proteins per NC disc filter. In addition, adsorption on NC stabilizes the Na,K-ATPase, since the activity was substantial 72 h after adsorption at 20 degreesC, After adsorption. the sensitivity of the enzyme to HgCl2 and CdCl2 inhibition was higher, The results show that immobilized Na,K-NTPase SPM can be used as a practical model for the detection of metal ions in different samples., Delimično prečišćena Na,K-ATPaza sinaptičkih plazma–membrana (SPM) mozga pacova imobilizovana je adsorpcijom na nitrocelulozne (NC) poliviniliden-fluorid (PVDF) membrane i membrane od staklenih vlakana (SV). Aktivnost enzima delimično prečišćenog solubilizacijom SDS-om povećana je oko 40%. Najveći procenat aktivnosti (14%) enzim zadržava posle 30 minuta adsorpcije SPM na 20ºC, pod vakuumom, sa 25 μg proteina po nitroceluloznom disku. Na,K-ATPaza imobilizovana na nitroceluloznoj membrani stabilna je 72 sata na 20ºC. Adsorpcijom, osetljivost enzima na inhibiciju Hg2+ i Cd2+ se povećava. Rezultati pokazuju da se imobilizovana Na,K-ATPaza SPM može koristiti za detekciju toksičnih metalnih jona u različitim uzorcima.", publisher = "Serbian Chemical Soc, Belgrade", journal = "Journal of the Serbian Chemical Society", title = "Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes, Imobilizacija Na,K-ATPpaze izolovane iz sinaptičkih plazma-membrana mozga pacova", volume = "67", number = "12", pages = "809-817", doi = "10.2298/JSC0212809M" }
Momic, T., Vujčić, Z., Vasic, V.,& Horvat, A.. (2002). Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes. in Journal of the Serbian Chemical Society Serbian Chemical Soc, Belgrade., 67(12), 809-817. https://doi.org/10.2298/JSC0212809M
Momic T, Vujčić Z, Vasic V, Horvat A. Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes. in Journal of the Serbian Chemical Society. 2002;67(12):809-817. doi:10.2298/JSC0212809M .
Momic, T, Vujčić, Zoran, Vasic, V, Horvat, A, "Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes" in Journal of the Serbian Chemical Society, 67, no. 12 (2002):809-817, https://doi.org/10.2298/JSC0212809M . .