Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae
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2022
Authors
Gođevac, Dejan
Ivanović, Stefan

Simić, Katarina

Anđelković, Boban D.

Jovanović, Živko S.

Rakić, Tamara
Article (Published version)

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Introduction Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated. Objective To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process Methods Proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates. Results Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved in the partia...lly rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples. Conclusion Using 1H-NMR and GC–MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants.
Keywords:
GC–MS / metabolomics / NMR / Ramonda / resurrection plantSource:
Phytochemical Analysis, 2022, 33, 6, 961-970Publisher:
- Wiley
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200178 (University of Belgrade, Faculty of Biology) (RS-200178)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) (RS-200026)
DOI: 10.1002/pca.3151
ISSN: 0958-0344
WoS: 00081078340000
Scopus: 2-s2.0-85131833166
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.3151http://cherry.chem.bg.ac.rs/handle/123456789/5417
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Gođevac, Dejan AU - Ivanović, Stefan AU - Simić, Katarina AU - Anđelković, Boban D. AU - Jovanović, Živko S. AU - Rakić, Tamara PY - 2022 UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.3151 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5417 AB - Introduction Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated. Objective To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process Methods Proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates. Results Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved in the partially rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples. Conclusion Using 1H-NMR and GC–MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants. PB - Wiley T2 - Phytochemical Analysis T2 - Phytochemical Analysis T1 - Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae VL - 33 IS - 6 SP - 961 EP - 970 DO - 10.1002/pca.3151 ER -
@article{ author = "Gođevac, Dejan and Ivanović, Stefan and Simić, Katarina and Anđelković, Boban D. and Jovanović, Živko S. and Rakić, Tamara", year = "2022", abstract = "Introduction Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated. Objective To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process Methods Proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates. Results Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved in the partially rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples. Conclusion Using 1H-NMR and GC–MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants.", publisher = "Wiley", journal = "Phytochemical Analysis, Phytochemical Analysis", title = "Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae", volume = "33", number = "6", pages = "961-970", doi = "10.1002/pca.3151" }
Gođevac, D., Ivanović, S., Simić, K., Anđelković, B. D., Jovanović, Ž. S.,& Rakić, T.. (2022). Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae. in Phytochemical Analysis Wiley., 33(6), 961-970. https://doi.org/10.1002/pca.3151
Gođevac D, Ivanović S, Simić K, Anđelković BD, Jovanović ŽS, Rakić T. Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae. in Phytochemical Analysis. 2022;33(6):961-970. doi:10.1002/pca.3151 .
Gođevac, Dejan, Ivanović, Stefan, Simić, Katarina, Anđelković, Boban D., Jovanović, Živko S., Rakić, Tamara, "Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae" in Phytochemical Analysis, 33, no. 6 (2022):961-970, https://doi.org/10.1002/pca.3151 . .