Horseradish peroxidase C1A wild type gene and its variants expressed in Pichia pastoris KM71H strain
Authors
Balaž, Ana Marija
Crnoglavac Popović, Milica
Stanišić, Marija
Ristić, Predrag

Senćanski, Milan

Todorović, Tamara

Prodanović, Radivoje

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Enzyme immobilization enables maintenance of enzyme activity and structural stability even in adverse conditions 1. Structural changes in enzymes that can occur due to the action of organic solvents, inhibitors or increased temperature can be prevented by immobilization of the enzymes in metal–organic frameworks (MOFs). It is reported that several enzymes, such as cytochrome c and horseradish peroxidase (HRP) have been successfully incorporated into MOFs 2. The aim of this work is to produce wild type horseradish peroxidase, isoform C1A, and several mutants specially designed to increase the activity and stability of HRP while immobilized within selected MOFs. Wild type and its variants were produced in metalotrophic yeast, Pichia pastoris KM71H strain, their activity and basic kinetic parameters were determined and compared prior imobilization.
Keywords:
horseradish peroxidase / mutations / kinetic parametersSource:
Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac, 2021, 49-49Funding / projects:
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Hemijski fakultetTY - CONF AU - Balaž, Ana Marija AU - Crnoglavac Popović, Milica AU - Stanišić, Marija AU - Ristić, Predrag AU - Senćanski, Milan AU - Todorović, Tamara AU - Prodanović, Radivoje PY - 2021 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5747 AB - Enzyme immobilization enables maintenance of enzyme activity and structural stability even in adverse conditions 1. Structural changes in enzymes that can occur due to the action of organic solvents, inhibitors or increased temperature can be prevented by immobilization of the enzymes in metal–organic frameworks (MOFs). It is reported that several enzymes, such as cytochrome c and horseradish peroxidase (HRP) have been successfully incorporated into MOFs 2. The aim of this work is to produce wild type horseradish peroxidase, isoform C1A, and several mutants specially designed to increase the activity and stability of HRP while immobilized within selected MOFs. Wild type and its variants were produced in metalotrophic yeast, Pichia pastoris KM71H strain, their activity and basic kinetic parameters were determined and compared prior imobilization. C3 - Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac T1 - Horseradish peroxidase C1A wild type gene and its variants expressed in Pichia pastoris KM71H strain SP - 49 EP - 49 UR - https://hdl.handle.net/21.15107/rcub_cherry_5747 ER -
@conference{ author = "Balaž, Ana Marija and Crnoglavac Popović, Milica and Stanišić, Marija and Ristić, Predrag and Senćanski, Milan and Todorović, Tamara and Prodanović, Radivoje", year = "2021", abstract = "Enzyme immobilization enables maintenance of enzyme activity and structural stability even in adverse conditions 1. Structural changes in enzymes that can occur due to the action of organic solvents, inhibitors or increased temperature can be prevented by immobilization of the enzymes in metal–organic frameworks (MOFs). It is reported that several enzymes, such as cytochrome c and horseradish peroxidase (HRP) have been successfully incorporated into MOFs 2. The aim of this work is to produce wild type horseradish peroxidase, isoform C1A, and several mutants specially designed to increase the activity and stability of HRP while immobilized within selected MOFs. Wild type and its variants were produced in metalotrophic yeast, Pichia pastoris KM71H strain, their activity and basic kinetic parameters were determined and compared prior imobilization.", journal = "Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac", title = "Horseradish peroxidase C1A wild type gene and its variants expressed in Pichia pastoris KM71H strain", pages = "49-49", url = "https://hdl.handle.net/21.15107/rcub_cherry_5747" }
Balaž, A. M., Crnoglavac Popović, M., Stanišić, M., Ristić, P., Senćanski, M., Todorović, T.,& Prodanović, R.. (2021). Horseradish peroxidase C1A wild type gene and its variants expressed in Pichia pastoris KM71H strain. in Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac, 49-49. https://hdl.handle.net/21.15107/rcub_cherry_5747
Balaž AM, Crnoglavac Popović M, Stanišić M, Ristić P, Senćanski M, Todorović T, Prodanović R. Horseradish peroxidase C1A wild type gene and its variants expressed in Pichia pastoris KM71H strain. in Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac. 2021;:49-49. https://hdl.handle.net/21.15107/rcub_cherry_5747 .
Balaž, Ana Marija, Crnoglavac Popović, Milica, Stanišić, Marija, Ristić, Predrag, Senćanski, Milan, Todorović, Tamara, Prodanović, Radivoje, "Horseradish peroxidase C1A wild type gene and its variants expressed in Pichia pastoris KM71H strain" in Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac (2021):49-49, https://hdl.handle.net/21.15107/rcub_cherry_5747 .